Difference between revisions of "Biosafety"

Latest revision as of 21:43, 21 April 2020

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More information about biosafety

• Quick links of pubmed research :

1. [http://www.ncbi.nlm.nih.gov/pubmed?term=biosafety%20gmo Biosafety and GMO's]
2. [http://www.ncbi.nlm.nih.gov/pubmed?term=biosafety%20synthetic%20biology Biosafety in Synthetic Biology]
3. [http://www.ncbi.nlm.nih.gov/pubmed?term=issue%20synthetic%20biology Issues of Synthetic Biology]

We encourage you to do this kind of research as part of your bibliography for whatever project you are working on.

• Here is the link to an openwetware page that should be improved by the community. This report exists to improve biosafety practices in synthetic biology, raise good questions, and encourage collaboration in exploring the issue.

-> [http://openwetware.org/wiki/How_safe_is_safe_enough:_towards_best_pratices_of_synthetic_biology How safe is Safe Enough?]

• Link to the biosafety recommendation page of iGEM:

Safety page

It includes book, papers and other useful references.

Recommendation from Human Practice Teams

• Report of Sara Aguiton : [http://2009.igem.org/Team:Paris/Ethics_ethicalreport#top report]
• Report of Claire Mayer : [http://2012.igem.org/Team:Paris_Bettencourt/Human_Practice/Report report]

Proposals to improve this page (Feel free to add ideas)

1. Add a biosafety grade, that could quantify the degree of robustness. For instance, in semantic containment with amber mutation, we score the robustness with the number of amber mutations.

Example of biosafety systems

Here are reported biosafety systems that teams created. The risk and hazards of these systems are assessed through fault tree analysis, a top-down approach used in engineering field.

Team	Fault Tree Analysis	Guesstimation
Paris Bettencourt 2012		Notation Failure component Failure mode Consequence Probability P1 physical containment leakage DNA/cell escape [http://2012.igem.org/Team:Paris_Bettencourt/Encapsulation#Cell_Containment_Assay experiment], [http://2012.igem.org/Team:Paris_Bettencourt/Modeling#Physical_containment_failure estimation : 4.10-6] P2 DNA uptake DNA transferred into natural strain HGT transconjugant to donor ratio in HGT is typically < 10-5 http://2012.igem.org/Team:Paris_Bettencourt/Modeling#References 4, assume this is the rate per generation P3 semantic containment genetic failure HGT With 3 amber mutations, probability to recover at least 50% of efficiency : 10-18 P4 population suicide no surrounding cells with enough toxin no death not estimated : consider it as 1 meanwhile P5 toxin production genetic failure no DNA degradation (w/o antitoxin), no death (with antitoxin) [http://2012.igem.org/Team:Paris_Bettencourt/Modeling#Genetic_failure estimation] : 10-6 P6 mutant fitness beneficial mutation out-competition of the mutant not determined, considered as 1. P7 delay system genetic failure no antitoxin degradation [http://2012.igem.org/Team:Paris_Bettencourt/Modeling#Genetic_failure estimation] : 10-6 P8 restriction enzyme genetic failure no antitoxin degradation [http://2012.igem.org/Team:Paris_Bettencourt/Modeling#Genetic_failure estimation] : 10-6 P9 restriction site genetic failure no antitoxin degradation 10-9

Semantic Containment

Here are parts that would add a semantic containment to genes, or parts that would allow the cell to read semantic containments gene.

Kill switch

Here are parts that could trigger cell death through disruption at the level of the protein and the cell.

XNase

Here are parts that could trigger cell death through disruption at the level of the DNA or RNA.

All biosafety parts

Include previous parts plus other marked as biosafety only.

Physical Containment

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