Composite

Part:BBa_K1639022

Designed by: Mustafa Yılmaz   Group: iGEM15_ATOMS-Turkiye   (2015-09-18)

pCons-TetR-pTet-TevProtease Suicide Switch

This is a kill switch part of our engineered E. Coli. pTet is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc. In the presence of aTc TetR repression inhibited and Tev Protease produce by Ptet promoter. Tev Protease release Pexiganan from DAMP and E. Coli kill switch works succesfully.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 798
    Illegal XhoI site found at 1540
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1401


[edit]
Categories
//biosafety/kill_switch
//chassis/prokaryote/ecoli
Parameters
None