Part:BBa_K4133012

LysKB317 (Endolysin) for Lactobacillus casei

LysKB317 is a novel recombinant peptidoglycan hydrolase (endolysin) derived from Lactobacillus bacteriophage vB_LfeS_EcoSau, with the ability of inhibiting the growth of lactic acid bacteria (Lu et al., 2020)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 615
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

Endolysins are enzymes produced by bacteriophages to degrade host bacterium cell walls, specifically the peptidoglycan. Due to this activity and specificity against bacteria, they are considered potential alternatives to antibiotics. LysKB317 (from Lactobacillus bacteriophage vB_LfeS_EcoSau) was described by Lu et al. (2020) in which they studied its growth inhibition activity against Lactobacillus species and demonstrated the effectiveness robust antibacterial activity against Lactobacillus. They reported 1 µM endolysin reduced bacterial load by 3-log fold change.

References

Lu, S. Y., Bischoff, K. M., Rich, J. O., Liu, S., & Skory, C. D. (2020). Recombinant bacteriophage LysKB317 endolysin mitigates Lactobacillus infection of corn mash fermentations. Biotechnology for Biofuels, 13(1). https://doi.org/10.1186/s13068-020-01795-9