DNA

Part:BBa_K2919000

Designed by: Evan Harrison   Group: iGEM19_MichiganState   (2019-10-04)


ghoT toxin from E. coli K12

The ghoT gene is the toxin half of the ghoST toxin-antitoxin system. This is a Type V toxin-antitoxin system where the antitoxin forms a protein that cleaves the mRNA of the toxin. When expressed the ghoT toxin forms a short helical transmembrane protein that creates pores in lipid bilayers. The pores in the membranes prevent a proton gradient from being built up and therefore prevent energy production in the cell causing slow growth or even cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None