Composite

Part:BBa_K2757000

Designed by: Bhuvana Sudarshan   Group: iGEM18_Oxford   (2018-10-10)


Art-175 fused with N-terminal DsbA 2-19 signal peptide and unidirectional pTet

This part consists of the artilysin Art-175 with a DsbA signal sequence under the influence of a unidirectional pTet promoter. Intracellular production of antimicrobial Art-175 commences upon induction of the tetracycline-regulated promoter. The DsbA secretion tag facilitates cotranslational export of Art-175 into the periplasmic space via the SRP pathway, promoting subsequent lysis of the host cell. The part was designed as a kill switch to promote host cell lysis upon interaction with a synthetic TetR inducer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 477
    Illegal AgeI site found at 676
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None