Part:BBa_K4139001

mcyH ABC Transporter for Microcystin Production (Composite)

The mcyH gene is a member of the mcy gene cluster in Microcystis aeruginosa cyanobacteria. The mcy gene cluster is responsible for the production of microcystins, which are hepatotoxins produced by the bacteria. The mcyH gene encodes an ATP binding cassette (ABC) transporter, which is essential for microcystin production. This part was designed so that the mcyH gene could be expressed in E.coli; mcyH is the target sequence for a Cas13a crRNA designed to target microcystis aeruginosa, so by designing a version of this part to be expressed in E.coli, we can test the target specificity of our Cas13a and crRNA in the lab without needing to work with toxic cyanobacteria.

This composite part includes the T7 promoter J64997, RBS B0034, and double terminator B0015.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 789
  Illegal AgeI site found at 113
  Illegal AgeI site found at 1177
  
• 1000
  COMPATIBLE WITH RFC[1000]