Composite

Part:BBa_K2486026

Designed by: Tiago Lubiana Alves   Group: iGEM17_USP-Brazil   (2017-10-28)


pGadA-MazF kill switch

[not sent]

Kill switch designed to reduce viability of E. coli upon contact with low pH environments in the range of normal rain drops (5-6).

pGadA promoter was characterized by team Dundee 2016 (BBa_K1962013) with GFP protein in different pH ranges. The GPF coding sequence was substituted by MazF, a endoribonuclease well characterized in kill switch approaches (1). This could limit replication of chassis for paratransgenesis in the environment, while allowing it to thrive inside the mosquito midgut.


  • Chan, Clement TY, et al. "'Deadman'and'Passcode'microbial kill switches for bacterial containment." Nature chemical biology 12.2 (2016): 82-86.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None