Composite
TetR repre

Part:BBa_K3317061

Designed by: Jorge Luis Hernandez Ayala   Group: iGEM19_UANL   (2019-10-16)


tetR repressed killswitch

Medium strength promoter that is constitutively on and repressed by TetR. The system expresses lysis proteins NucA, NucD, and NucE along with RBS specifically designed for ethe overexpression of each CDS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 649
    Illegal PstI site found at 1386
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 649
    Illegal PstI site found at 1386
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1390
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 649
    Illegal PstI site found at 1386
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 649
    Illegal PstI site found at 1386
    Illegal NgoMIV site found at 719
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
//biosafety/kill_switch
//cds/lysis
//cds/membrane/lysis
Parameters
None