Part:BBa_K3096052

CRISPR Array targeting the Plasmid DNA of the glucose dependent Exendin-4 expression system

This CRISPR array codes for the gRNAs that target the plasmid which encodes the glucose dependent Exendin-4 expression system and is part of the CRISPR/Cas3 kill switch system to prevent the release of the probiotic. The gRNA will guide the Cas3 Nuclease to the plasmid DNA which will then be degraded.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Xia, Peng-Fei, et al. "Synthetic genetic circuits for programmable biological functionalities." Biotechnology advances (2019).
2. Terns, Michael P., and Rebecca M. Terns. "CRISPR-based adaptive immune systems." Current opinion in microbiology 14.3 (2011): 321-327.