Part:BBa_K515106

J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806

A composite part comprising of a J23100 promotor, holin (BBa_K112805) and endolysin (BBa_K112806). This part has been to designed to lyse any cell which does not contain part BBa_K515104 in the genome.

Warning: This part is not RFC10 compatible

During Imperial College London's 2011 iGEM team's assembly of Gene Guard they encountered problems when cloning the holin-endolysin construct. They have found that there is an illegal XbaI site in the T4 Holin protein sequence originating from BBa_K112808. This was also found in the HQ's sequencing results but the RFC10 compatibility tag has not been removed from the front page of this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 616
  Illegal AgeI site found at 728
  Illegal AgeI site found at 1730
  Illegal AgeI site found at 1800
  
• 1000
  COMPATIBLE WITH RFC[1000]