Coding

Part:BBa_K4202001

Designed by: Zhijian Yan   Group: iGEM22_ZJU-China   (2022-09-24)


mazF(codon optimization for Bacillus subtilis)

Encodes the stable toxin mazF in E.coli. Codon has been optimized for the expression in Bacillus subtilis. It is used in conjungtion with mazE in our chassis (Bacillus subtilis) to provide a toxin-antitoxin kill swich to prevent the escape of engineered bacteria in to environment in certain stressful condictions. When the expression of mazE and mazF is turned off (under control of the same promoter), mazE will be degraded faster than mazF. Non-specific ribonucleic acid endonuclease activity of mazF will kill the engineered bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 158
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 371


[edit]
Categories
//biosafety/kill_switch
//cds
//chassis/prokaryote/bsubtilis
Parameters
None