Composite

Part:BBa_K3317060

Designed by: Jorge Luis Hernandez Ayala   Group: iGEM19_UANL   (2019-10-16)


NucD

Optimized RBS + CDS. The RBS designed specifically for NucD (Putative phage lysozyme ) overexpression in E.coli K12, with a predicted translation initiation rate of 127853.75

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 80
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 80
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 80
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 80
    Illegal NgoMIV site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
//biosafety/kill_switch
//cds/lysis
//cds/membrane/lysis
Parameters
None