Part:BBa_K4133006
Repressor protein CtsR_Lp (Lactobacillus plantarum WCFS1) for Lactobacillus casei
Repressor protein from Lactobacillus plantarum WCFS1 capable of binding to the BBa_K4133007 repressible promoter to inhibit its function.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The repressor systems that Fiocco et al. (2010) found in L. plantarum is explained by the most studied clpC operon in Bacillus subtilis, which behave similarly, according to their studies, in these putative regions for each specie. The clpC operon is regulated by a class III stress induction mechanism in which the first gene ctsR, containing a predicted helix-turn-helix DNA-binding motif, acts as a negative regulator for a PclpC promoter. The deletion of the ctsR gene ended up in the high expression of the clpC operon under unstressed conditions, but once they tried the wildtype system under stress conditions like high temperatures (over 42˚C) a further increase in clpC operon was found, demonstrating that the ctsR protein acts as a global repressor and gets inactivated or dissociated under inducing stress conditions like 42˚C (Krüger et al., 1998).
References
Krüger, E., & Hecker, M. (1998). The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes. Journal of bacteriology, 180(24), 6681–6688. https://doi.org/10.1128/JB.180.24.6681-6688.1998
Fiocco, D., Capozzi, V., Collins, M., Gallone, A., Hols, P., Guzzo. J., Weidmann, S., Rieu. A., Msadek, T. & Spano, G. (2010). Characterization of the CtsR Stress Response Regulon in Lactobacillus plantarum. Journal of Bacteriology, 192(3): 896-900. https://doi.org/10.1128/JB.01122-09
//biosafety/kill_switch
//cds
//cds/transcriptionalregulator/repressor
//chassis/prokaryote/lactobacillus
//collections/probiotics
| protein |