Composite

Part:BBa_K3273010

Designed by: Bruno Batista, Fernando de Souza, Isabelle Taira, Lucas Boldrini   Group: iGEM19_Sao_Carlos-Brazil   (2019-10-15)


Composite MET 25 nucA

This composite part consists of the BBa_K165000 methionine induced promoter, followed by the Kozak sequence BBa_J63003, S. aureus nucA nuclease BBa_K1159105 and CYC1 terminator BBa_K2637017. It was designed to be used as a kill-switch system controlled by methionine concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/kill_switch
Parameters
None