Part:BBa_K3755045

Hok/Mok protein

The toxic protein from Hok-Sok TA system of R1 plasmid of E.coli, inhibited by Sok gene.

Introduction of hok/sok system

The hok/sok system is a type I toxin-antitoxin (TA) system of E.coli. In type I TA systems, the antitoxin is a small antisense RNA that inhibits the translation of small toxic proteins by binding to the corresponding mRNAs. The hok/sok system loci mainly consists of three parts, hok (host killing,Part:BBa_K3755043), sok (suppression of killing) and mok (modulation of killing). The hok gene encodes a toxic transmembrane protein, which can damage the membrane of E.coli. The reading frame of mok overlaps extensively with hok, and is necessary for expression and regulation of hok translation. The sok gene encodes a small cis-acting antisense RNA of 64 nucleotides which can attach with the mok mRNA leader region and inhibit its translation. Through inhibit the translation of mok, sok indirectly inhibits the expression of toxic proteins, hok.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]