Regulatory

Part:BBa_K4133010

Designed by: Luis Alejandro Chaves Martinez   Group: iGEM22_Costa_Rica   (2022-10-04)


PclpC_Lc repressible promoter (Lactococcus lactis)

Repressible promoter to which the BBa_K4133009 repressor protein binds in order to inhibit its activity. This promoter was originally obtained from the Lactococcus lactis ctsR gene (GenBank Accession: AJ249133.1)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The repressor systems that Varmanen et al. (2000) found in L. lactis is explained by the most studied clpC operon in Bacillus subtilis, which behave similarly, according to their studies, in these putative regions for each species. The clpC operon is regulated by a class III stress induction mechanism in which the first gene ctsR, containing a predicted helix-turn-helix DNA-binding motif, acts as a negative regulator for a PclpC promoter. The deletion of the ctsR gene ended up in the high expression of the clpC operon under unstressed conditions, but once they tried the wildtype system under stress conditions like high temperatures (over 42˚C) a further increase in clpC operon was found, demonstrating that the ctsR protein acts as a global repressor and gets inactivated or dissociated under inducing stress conditions like 42˚C (Krüger et al., 1998).


References

Krüger, E., & Hecker, M. (1998). The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes. Journal of bacteriology, 180(24), 6681–6688. https://doi.org/10.1128/JB.180.24.6681-6688.1998

Varmanen, P., Ingmer, H. & Vogensen, F. K. (2000). CtsR of Lactococcus lactis encodes a negative regulator of clp gene expression. Microbiology, 146: 1447–1455. https://doi.org/10.1099/00221287-146-6-1447

[edit]
Categories
//awards/basic_part
//biosafety/kill_switch
//chassis/prokaryote/lactobacillus
//collections/probiotics
//promoter
//regulation/negative
Parameters
negative_regulators