Biosafety
Semantic Containment
Here are parts that would add a semantic containment to genes, or parts that would allow the cell to read semantic containments gene.
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1479017 | Protein_Domain | just for test | 100 |
| BBa_K1479018 | Protein_Domain | Just for test | |
| BBa_K2086001 | Coding | Serine Repeat Antigen (SerA) | 1233 |
| BBa_K914000 | RNA | pLac-supD-T | 241 |
| BBa_K914009 | Translational_Unit | P1003* Ser133->Amber Codon | 967 |
| BBa_K914011 | Intermediate | supD-T - intermediate | 178 |
| BBa_K914012 | Plasmid_Backbone | pSB1A2 with 1 amber mutation | 2079 |
| BBa_K914018 | Translational_Unit | P1003** Kan resistant gene with 2 Amber Codon | 967 |
Kill switch
Here are parts that could trigger cell death through disruption at the level of the protein and the cell.
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1074011 | Composite | PsdpRI+RBS+SDP | 2207 |
| BBa_K117000 | Coding | Lysis gene (promotes lysis in colicin-producing bacteria strain) | 144 |
| BBa_K1172901 | Coding | Alanine racemase from ''E. coli'' | 1080 |
| BBa_K1172902 | Device | alanine racemase (''alr'') under the control of the P''tac'' promoter | 1134 |
| BBa_K1172908 | Device | Part 1 of the Biosafety-System Lac of Growth (pRha lacI alr) | 2509 |
| BBa_K1172909 | Device | Biosafety-System araCtive (AraC) | 3281 |
| BBa_K1172911 | Device | Biosafety-System Lac of Growth (lacI) | 3600 |
| BBa_K1172912 | Device | Part 1 of the Biosystem TetOR alive (pRha TetR alr) | 2042 |
| BBa_K1184000 | Coding | KillerRed | 723 |
| BBa_K1197007 | Composite | IPTG dependent B.subtilis Kill Switch construct | 2006 |
| BBa_K1223001 | Project | P.A.S.E. 1 cassette | 2148 |
| BBa_K1223002 | Project | P.A.S.E. 2 cassette | 1210 |
| BBa_K1405008 | Composite | A Kill Switch with "memory" time repressed by IPTG | 2926 |
| BBa_K1616005 | Coding | Holin/endolysin reversed - RBS and double T7 terminator | 1379 |
| BBa_K1616013 | Coding | ccdB reversed - RBS and double T7 terminator | 575 |
| BBa_K1639022 | Composite | pCons-TetR-pTet-TevProtease Suicide Switch | 1546 |
| BBa_K1767001 | Composite | P(Lac)IQ RBS Aiia RBS LuxR term term luxpR | 1818 |
| BBa_K1767002 | Composite | P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter | 2666 |
| BBa_K1767003 | Composite | P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter | 3597 |
| BBa_K1767004 | Composite | P(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter | 1892 |
| BBa_K1819008 | Device | Promoter test circuit to analyze the kill switch efficiency | 2725 |
| BBa_K1820024 | Generator | Phage inducible promoter 1997 +RBS+FspI + term | 1160 |
| BBa_K1820025 | Generator | p2001-native RBS-Fsp1-B0015 | 1209 |
| BBa_K1914000 | Coding | E. coli Codon Optimised KillerOrange(EOKO) | 717 |
| BBa_K1914001 | Device | pT7- E. coli optimised - KillerOrange (EOKO) | 926 |
| BBa_K1914003 | Device | pT7- E. coli optimised - KillerRed (EOKR) | 929 |
| BBa_K1919100 | Device | The device consist of temperature promotor and hokD coding gene. | 1112 |
| BBa_K2040122 | Coding | KillerRed + NLS | 744 |
| BBa_K2086002 | Composite | PyeaR - SerA | 1499 |
| BBa_K2150103 | Coding | 1204 toxin CDS | 507 |
| BBa_K2150104 | Coding | 6249 toxin CDS | 319 |
| BBa_K2232025 | Composite | mazEF switch | 1006 |
| BBa_K2292002 | Coding | Toxin-antitoxin mazEF | 584 |
| BBa_K2292006 | Composite | MazEF kill switch | 877 |
| BBa_K2486026 | Composite | pGadA-MazF kill switch | 769 |
| BBa_K2616002 | Coding | Temperature Kill Switch | 938 |
| BBa_K2660006 | Coding | N-Cas9 | 3459 |
| BBa_K2660007 | Coding | C-Cas9 | 648 |
| BBa_K2757000 | Composite | Art-175 fused with N-terminal DsbA 2-19 signal peptide and unidirectional pTet | 1008 |
| BBa_K2757001 | Composite | Art-175 fused with N-terminal DsbA 2-19 signal peptide and bidirectional pTet | 1065 |
| BBa_K2817006 | Composite | PcspA-RBS-mazF | 535 |
| BBa_K2896001 | Coding | Thylakoid-bound SuperNova (for S. elongatus PCC 7942) | 846 |
| BBa_K2919000 | DNA | ghoT toxin from E. coli K12 | 174 |
| BBa_K302034 | Translational_Unit | mazEF toxin-antitoxin cluster | 607 |
| BBa_K302035 | Device | Sucrose-limitation induced kill switch | 1006 |
| BBa_K3036004 | Composite | Cold triggered kill switch | 649 |
| BBa_K3036008 | Coding | RelB with degradation-promoting tag RepA | 1758 |
| BBa_K3096052 | RNA | CRISPR Array targeting the Plasmid DNA of the glucose dependent Exendin-4 expression system | 147 |
| BBa_K3096054 | Composite | Cas3 Nuclease under the control of the AraC promoter pBAD | 2803 |
| BBa_K3096055 | Composite | Type I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter | 4487 |
| BBa_K3190601 | Composite | pGAL1-BI-I: BI-I CDS under inducible promoter | 1181 |
| BBa_K3215013 | Composite | Tse2 Repressed by Arabinose Kill Switch | 1950 |
| BBa_K3215016 | Composite | MazF repressed by IPTG Kill Switch | 2886 |
| BBa_K3237029 | Composite | nucA | 1754 |
| BBa_K3273001 | Coding | Serratia marcescens nucA nuclease | 801 |
| BBa_K3273008 | Composite | Composite ADH2/TDH3 nucA | 1849 |
| BBa_K3273009 | Composite | Composite HXT6 Bax | 2039 |
| BBa_K3273010 | Composite | Composite MET 25 nucA | 1113 |
| BBa_K3317053 | Coding | NucD | 540 |
| BBa_K3317059 | Composite | NucE lysis construction | 308 |
| BBa_K3317060 | Composite | NucD | 572 |
| BBa_K3317061 | Composite | tetR repressed killswitch | 1504 |
| BBa_K3378002 | Regulatory | Ferrous iron sensetive promoter fhuA1 | 55 |
| BBa_K3512001 | Coding | ccdB Toxin | 306 |
| BBa_K3588014 | Composite | MazE-MazF Hypoxia induced kill switch | 2211 |
| BBa_K3634012 | Composite | Glucose-Mediated Death Sensor | 566 |
| BBa_K3755043 | RNA | Sok RNA | 66 |
| BBa_K3755045 | Coding | Hok/Mok protein | 217 |
| BBa_K3755046 | Composite | Double Regulated(trp+lac) Kill Switch | 2352 |
| BBa_K3848004 | Composite | Lactate regulated kill switch | 2837 |
| BBa_K4133006 | Coding | Repressor protein CtsR_Lp (Lactobacillus plantarum WCFS1) for Lactobacillus casei | 468 |
| BBa_K4133007 | Regulatory | PclpC_Lp repressible promoter (Lactobacillus plantarum WCFS1) | 59 |
| BBa_K4133008 | RBS | RBS_Lp (Lactobacillus plantarum WCFS1) | 14 |
| BBa_K4133009 | Coding | Repressor protein CtsR_Lc (Lactococcus lactis) for Lactobacillus casei | 456 |
| BBa_K4133010 | Regulatory | PclpC_Lc repressible promoter (Lactococcus lactis) | 72 |
| BBa_K4133011 | RBS | RBS_Lc (Lactococcus lactis) | 12 |
| BBa_K4133012 | Coding | LysKB317 (Endolysin) for Lactobacillus casei | 894 |
| BBa_K4202000 | Coding | mazE(codon optimization for Bacillus subtilis) | 249 |
| BBa_K4202001 | Coding | mazF(codon optimization for Bacillus subtilis) | 421 |
| BBa_K4202045 | Generator | Sucrose regulated kill switch in Bacillus subtilis | 1291 |
| BBa_K4345020 | Composite | 5' UTR of hsp17 with ccdA fused to sfGFP | 1197 |
| BBa_K4345023 | Composite | 5' UTR of rpoH with ccdA fused to sfGFP | 1395 |
| BBa_K4345024 | Composite | 5' UTR of prfA with ccdA fused to sfGFP | 1277 |
| BBa_K515104 | Composite | J23100 promoter - Antiholin | 488 |
| BBa_K515106 | Composite | J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806 | 1973 |
| BBa_K5480009 | Composite | Y38-DpnI-TrrnB | 2353 |
| BBa_K628006 | Composite | Protegrin-1 Kill Switch | 353 |
| BBa_K879917 | Plasmid_Backbone | LacO ori | 2072 |
| BBa_K879918 | Plasmid_Backbone | TetO ori | 2074 |
XNase
Here are parts that could trigger cell death through disruption at the level of the DNA or RNA.
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1172904 | Coding | Rnase Ba (Barnase) from Bacillus amyloliquefaciens | 339 |
| BBa_K131000 | Generator | ColE2 Operon | 2640 |
| BBa_K1479016 | Promoter | -- No description -- | |
| BBa_K1479017 | Protein_Domain | just for test | 100 |
| BBa_K150009 | Generator | ColicinE1 Producer Controlled by 3OC6HSL Receiver Device | 3180 |
| BBa_K526001 | Coding | ptrc*-Dpn | 1552 |
| BBa_K914001 | Composite | pLac Colicin E2 Immunity protein | 345 |
| BBa_K914002 | Intermediate | repressilator RBS Colicin E2 Immunity protein | 282 |
Physical Containment
