Biosafety

Revision as of 09:05, 3 October 2012 by Jeancury (Talk | contribs) (More information about biosafety)

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More information about biosafety

  • Quick links of pubmed research :
  1. [http://www.ncbi.nlm.nih.gov/pubmed?term=biosafety%20gmo biosafety gmo]
  1. [http://www.ncbi.nlm.nih.gov/pubmed?term=biosafety%20synthetic%20biology biosafety synthetic biology]
  1. [http://www.ncbi.nlm.nih.gov/pubmed?term=issue%20synthetic%20biology issue synthetic biology]

We encourage you to do this kind of research as part of your bibliography for whatever research you are working on.

  • Here is the link an openwetware page that aims at being improved by the community. The idea is to improve biosafety practices in synthetic biology, through this report, trying to raise the good questions, all together.

->[http://openwetware.org/wiki/How_safe_is_safe_enough:_towards_best_pratices_of_synthetic_biology How safe is safe enough?]

  • The link toward the biosafety recommendation page of iGEM:

[igem.org/safety Safety page]

Semantic Containment

Here are parts that would add a semantic containment to genes, or parts that would allow the cell to read semantic containments gene.


More...
NameTypeDescriptionLength
BBa_K1479017Protein_Domainjust for test100
BBa_K1479018Protein_DomainJust for test 
BBa_K2086001CodingSerine Repeat Antigen (SerA)1233
BBa_K914000RNApLac-supD-T241
BBa_K914009Translational_UnitP1003* Ser133->Amber Codon967
BBa_K914011IntermediatesupD-T - intermediate178
BBa_K914012Plasmid_BackbonepSB1A2 with 1 amber mutation2079
BBa_K914018Translational_UnitP1003** Kan resistant gene with 2 Amber Codon967


Kill switch

Here are parts that could trigger cell death through disruption at the level of the protein and the cell.


More...
NameTypeDescriptionLength
BBa_K1074011CompositePsdpRI+RBS+SDP2207
BBa_K117000CodingLysis gene (promotes lysis in colicin-producing bacteria strain)144
BBa_K1172901CodingAlanine racemase from ''E. coli''1080
BBa_K1172902Devicealanine racemase (''alr'') under the control of the P''tac'' promoter1134
BBa_K1172908DevicePart 1 of the Biosafety-System Lac of Growth (pRha lacI alr)2509
BBa_K1172909DeviceBiosafety-System araCtive (AraC)3281
BBa_K1172911DeviceBiosafety-System Lac of Growth (lacI)3600
BBa_K1172912DevicePart 1 of the Biosystem TetOR alive (pRha TetR alr)2042
BBa_K1184000CodingKillerRed723
BBa_K1197007CompositeIPTG dependent B.subtilis Kill Switch construct2006
BBa_K1223001ProjectP.A.S.E. 1 cassette2148
BBa_K1223002ProjectP.A.S.E. 2 cassette1210
BBa_K1405008CompositeA Kill Switch with "memory" time repressed by IPTG2926
BBa_K1616005CodingHolin/endolysin reversed - RBS and double T7 terminator1379
BBa_K1616013CodingccdB reversed - RBS and double T7 terminator575
BBa_K1639022CompositepCons-TetR-pTet-TevProtease Suicide Switch1546
BBa_K1767001CompositeP(Lac)IQ RBS Aiia RBS LuxR term term luxpR1818
BBa_K1767002CompositeP(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter2666
BBa_K1767003CompositeP(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter 3597
BBa_K1767004CompositeP(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter1892
BBa_K1819008DevicePromoter test circuit to analyze the kill switch efficiency2725
BBa_K1820024GeneratorPhage inducible promoter 1997 +RBS+FspI + term1160
BBa_K1820025Generatorp2001-native RBS-Fsp1-B00151209
BBa_K1914000CodingE. coli Codon Optimised KillerOrange(EOKO)717
BBa_K1914001DevicepT7- E. coli optimised - KillerOrange (EOKO)926
BBa_K1914003DevicepT7- E. coli optimised - KillerRed (EOKR)929
BBa_K1919100DeviceThe device consist of temperature promotor and hokD coding gene.1112
BBa_K2040122CodingKillerRed + NLS744
BBa_K2086002CompositePyeaR - SerA1499
BBa_K2150103Coding1204 toxin CDS507
BBa_K2150104Coding6249 toxin CDS319
BBa_K2232025CompositemazEF switch1006
BBa_K2292002CodingToxin-antitoxin mazEF584
BBa_K2292006CompositeMazEF kill switch 877
BBa_K2486026CompositepGadA-MazF kill switch 769
BBa_K2616002CodingTemperature Kill Switch938
BBa_K2660006CodingN-Cas93459
BBa_K2660007CodingC-Cas9648
BBa_K2757000CompositeArt-175 fused with N-terminal DsbA 2-19 signal peptide and unidirectional pTet1008
BBa_K2757001CompositeArt-175 fused with N-terminal DsbA 2-19 signal peptide and bidirectional pTet1065
BBa_K2817006CompositePcspA-RBS-mazF535
BBa_K2896001CodingThylakoid-bound SuperNova (for S. elongatus PCC 7942)846
BBa_K2919000DNAghoT toxin from E. coli K12174
BBa_K302034Translational_UnitmazEF toxin-antitoxin cluster607
BBa_K302035DeviceSucrose-limitation induced kill switch1006
BBa_K3036004CompositeCold triggered kill switch649
BBa_K3036008CodingRelB with degradation-promoting tag RepA1758
BBa_K3096052RNACRISPR Array targeting the Plasmid DNA of the glucose dependent Exendin-4 expression system147
BBa_K3096054CompositeCas3 Nuclease under the control of the AraC promoter pBAD2803
BBa_K3096055CompositeType I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter4487
BBa_K3190601CompositepGAL1-BI-I: BI-I CDS under inducible promoter1181
BBa_K3215013CompositeTse2 Repressed by Arabinose Kill Switch1950
BBa_K3215016CompositeMazF repressed by IPTG Kill Switch2886
BBa_K3237029CompositenucA1754
BBa_K3273001CodingSerratia marcescens nucA nuclease801
BBa_K3273008CompositeComposite ADH2/TDH3 nucA1849
BBa_K3273009CompositeComposite HXT6 Bax2039
BBa_K3273010CompositeComposite MET 25 nucA1113
BBa_K3317053CodingNucD540
BBa_K3317059CompositeNucE lysis construction308
BBa_K3317060CompositeNucD572
BBa_K3317061CompositetetR repressed killswitch1504
BBa_K3378002RegulatoryFerrous iron sensetive promoter fhuA155
BBa_K3512001CodingccdB Toxin306
BBa_K3588014CompositeMazE-MazF Hypoxia induced kill switch 2211
BBa_K3634012CompositeGlucose-Mediated Death Sensor566
BBa_K3755043RNASok RNA66
BBa_K3755045CodingHok/Mok protein217
BBa_K3755046CompositeDouble Regulated(trp+lac) Kill Switch2352
BBa_K3848004CompositeLactate regulated kill switch2837
BBa_K4133006CodingRepressor protein CtsR_Lp (Lactobacillus plantarum WCFS1) for Lactobacillus casei468
BBa_K4133007RegulatoryPclpC_Lp repressible promoter (Lactobacillus plantarum WCFS1)59
BBa_K4133008RBSRBS_Lp (Lactobacillus plantarum WCFS1)14
BBa_K4133009CodingRepressor protein CtsR_Lc (Lactococcus lactis) for Lactobacillus casei456
BBa_K4133010RegulatoryPclpC_Lc repressible promoter (Lactococcus lactis) 72
BBa_K4133011RBSRBS_Lc (Lactococcus lactis)12
BBa_K4133012CodingLysKB317 (Endolysin) for Lactobacillus casei894
BBa_K4202000CodingmazE(codon optimization for Bacillus subtilis)249
BBa_K4202001CodingmazF(codon optimization for Bacillus subtilis)421
BBa_K4202045GeneratorSucrose regulated kill switch in Bacillus subtilis1291
BBa_K4345020Composite5' UTR of hsp17 with ccdA fused to sfGFP1197
BBa_K4345023Composite5' UTR of rpoH with ccdA fused to sfGFP1395
BBa_K4345024Composite5' UTR of prfA with ccdA fused to sfGFP1277
BBa_K515104CompositeJ23100 promoter - Antiholin488
BBa_K515106CompositeJ23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K1128061973
BBa_K5480009CompositeY38-DpnI-TrrnB2353
BBa_K628006CompositeProtegrin-1 Kill Switch353
BBa_K879917Plasmid_BackboneLacO ori2072
BBa_K879918Plasmid_BackboneTetO ori2074


XNase

Here are parts that could trigger cell death through disruption at the level of the DNA or RNA.


More...
NameTypeDescriptionLength
BBa_K1172904CodingRnase Ba (Barnase) from Bacillus amyloliquefaciens339
BBa_K131000GeneratorColE2 Operon2640
BBa_K1479016Promoter -- No description --  
BBa_K1479017Protein_Domainjust for test100
BBa_K150009GeneratorColicinE1 Producer Controlled by 3OC6HSL Receiver Device3180
BBa_K526001Codingptrc*-Dpn1552
BBa_K914001CompositepLac Colicin E2 Immunity protein345
BBa_K914002Intermediaterepressilator RBS Colicin E2 Immunity protein282


Physical Containment