Difference between revisions of "Protein coding sequences/Reporters"
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<parttable>luciferase_reporter_protein_coding_sequence</parttable> | <parttable>luciferase_reporter_protein_coding_sequence</parttable> | ||
| − | <!-- To include a part in this table, include the categories "//cds/reporter/luciferase" and set the part type to Coding under the Hard Information tab of the part. --> | + | <!-- To include a part in this table, include the categories "//cds/reporter/luciferase" and "//cds/enzyme"and set the part type to Coding under the Hard Information tab of the part. --> |
==Enzymes that produce colored substrates== | ==Enzymes that produce colored substrates== | ||
Revision as of 22:28, 17 January 2009
< Back to Protein coding sequences
Reporter protein coding sequences are used to measure gene expression.
Contents
Fluorescent proteins
| Name | Protein | Description | Tag | Direction | Fluorescent Color | Emission | Excitation | Length | Status | Uses |
|---|---|---|---|---|---|---|---|---|---|---|
| BBa_E0030 | EYFP | enhanced yellow fluorescent protein derived from A. victoria GFP | None | Forward | Yellow | 527 | 514 | 723 | In stock | 140 |
| BBa_E0020 | ECFP | engineered cyan fluorescent protein derived from A. victoria GFP | None | Forward | Cyan | 476 | 439 | 723 | In stock | 95 |
| BBa_E1010 | mRFP1 | **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) | None | Forward | Red | 607 | 584 | 706 | In stock | 593 |
| BBa_E0040 | GFPmut3b | green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 | None | Forward | Green | 511 | 501 | 720 | In stock | 875 |
| BBa_E2050 | mOrange | derivative of mRFP1, yeast-optimized | None | Orange | 562 | 548 | 769 | In stock | 15 | |
| BBa_J52021 | dnTraf6-linker-GFP | Green | 1446 | In stock | 1 | |||||
| BBa_J52026 | dnMyD88-linker-GFP | Green | 1155 | In stock | 1 | |||||
| BBa_I715022 | Amino Portion of RFP | Red | 462 | In stock | 2 | |||||
| BBa_I715023 | Carboxyl portion of RFP | Red | 220 | In stock | 1 | |||||
| BBa_I712028 | CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence | Forward | Red | 733 | In stock | 8 | ||||
| BBa_K125500 | GFP fusion brick | Forward | Green | 718 | In stock | 13 | ||||
| BBa_K165005 | Venus YFP, yeast optimized for fusion | Forward | Yellow | 744 | In stock | 2 | ||||
| BBa_K082003 | GFP | GFP(+LVA) | Green | 756 | In stock | 31 | ||||
| BBa_K156009 | OFP (orange fluorescent protein) | Forward | Orange | 864 | In stock | 1 | ||||
| BBa_K156010 | SBFP2 (strongly enhanced blue fluorescent protein) | Blue | 720 | In stock | 9 | |||||
| BBa_K294055 | GFPmut3b | GFP RFP Hybrid | None | Green | 511 | 501 | 720 | In stock | 3 | |
| BBa_K283005 | lpp_ompA_eGFP_streptavidin | 1533 | In stock | 1 | ||||||
| BBa_K157043 | CFP | 732 | In stock | 1 | ||||||
| BBa_K411207 | Green Fluorescent Protein with LVA tag (GFPLVA) | Green | 753 | In stock | 2 | |||||
| BBa_K648013 | GFP with Standard 25 Prefix/Suffix | Green | 735 | In stock | 13 | |||||
| BBa_K525306 | Fusion Protein of S-Layer SgsE and mCerulean | Blue | 3129 | In stock | ||||||
| BBa_K592100 | Blue Fluorescent Protein (mTagBFP) | Blue | 705 | In stock | 41 | |||||
| BBa_K592101 | Yellow Fluorescent Protein (YFP) | Yellow | 720 | In stock | 5 | |||||
| BBa_K864100 | Super Yellow Fluorescent Protein 2 (SYFP2) | Yellow | 723 | In stock | 8 | |||||
| BBa_K1122005 | dsRED (BBa_E1010) compatible with Genabler assembly | Red | 697 | In stock | ||||||
| BBa_K1122006 | mCherry (BBa_J06504) compatible with Genabler assembly | Red | 730 | In stock | ||||||
| BBa_K1122008 | mTagBFP (BBa_K592100) compatible with Genabler assembly | Blue | 721 | In stock | ||||||
| BBa_K1033916 | amajLime, yellow-green chromoprotein | Yellow, Green | 693 | In stock | 10 | |||||
| BBa_K1184000 | KillerRed | Red | 723 | In stock | 5 | |||||
| BBa_K1159301 | Super Yellow Fluorescent Protein 2 (SYFP2) in RFC[25] | Yellow | 714 | In stock | 1 | |||||
| BBa_K1159302 | Enhanced Cyan Fluorescent Protein (eCFP) in RFC[25] | Cyan | 714 | In stock | 1 | |||||
| BBa_K1093002 | sfCFP | Cyan | 720 | In stock | ||||||
| BBa_K1093003 | sfBFP N-terminal | Blue | 645 | In stock | ||||||
| BBa_K1093004 | sfCFP N-terminal | Cyan | 645 | In stock | ||||||
| BBa_K1093017 | mCherry C-terminal | Red | 243 | In stock | ||||||
| BBa_K1399000 | mRFP1 | RFP from Discosoma striata (coral) with AAV-ssrA degradation tag | SsrA-AAV | Forward | Red | 607 | 584 | 714 | In stock | |
| BBa_K1399001 | mRFP1 | RFP from Discosoma striata (coral) with LVA-ssrA degradation tag | SsrA-LVA degradation tag | Forward | Red | 607 | 584 | 714 | In stock | 2 |
| BBa_K1399002 | mRFP1 | RFP from Discosoma striata (coral) with LAA-ssrA degradation tag (wt) | SsrA-LAA degradation tag | Forward | Red | 607 | 584 | 714 | In stock | 1 |
| BBa_K1399003 | mRFP1 | RFP from Discosoma striata (coral) with DAS-ssrA degradation tag | SsrA-DAS degradation tag | Forward | Red | 607 | 584 | 714 | In stock | 1 |
| BBa_K1399005 | GFPmut3b | GFP (mut3b) with AAV-ssrA degradation tag | SsrA-AAV degradation tag | Forward | Green | 511 | 501 | 753 | In stock | 4 |
| BBa_K1399004 | GFPmut3b | GFP (mut3b) with LVA-ssrA degradation tag | SsrA-LVA degradation tag | Forward | Green | 511 | 501 | 753 | In stock | 8 |
| BBa_K1399006 | GFPmut3b | GFP (mut3b) with LAA-ssrA degradation tag | SsrA-LAA degradation tag | Forward | Green | 511 | 501 | 753 | In stock | 2 |
| BBa_K1399007 | GFPmut3b | GFP (mut3b) with SsrA-DAS+2 degradation tag | SsrA-DAS+2 degradation tag | Forward | Green | 511 | 501 | 759 | In stock | 4 |
| BBa_K1399008 | GFPmut3b | GFP (mut3b) with DAS-ssrA degradation tag | SsrA-DAS degradation tag | Forward | Green | 511 | 501 | 753 | In stock | 2 |
| BBa_K1351021 | Monomeric Red Fluorescent Protein from Discosoma striata | Red | 675 | In stock | ||||||
| BBa_K1323009 | DsRed (RFP) Coding Sequence | Red | 678 | In stock | 4 | |||||
| BBa_K1323010 | YFP Coding Sequence | Yellow | 720 | In stock | ||||||
| BBa_K1510017 | red florescence protein RFC23 | Red | 703 | In stock | ||||||
| BBa_K1486056 | CxpR & Split IFP1.4 [Cterm + Cterm][2] | Forward | Infrared | 3742 | In stock | |||||
| BBa_K1321337 | sfGFP in Freiburg format (RFC 25) | Green | 711 | In stock | 27 | |||||
| BBa_K1418030 | SYFP2 | Yellow | 723 | In stock | ||||||
| BBa_K1418040 | CFP | Cyan | 723 | In stock | ||||||
| BBa_K1418041 | CFP(ns) | Cyan | 717 | In stock | ||||||
| BBa_K1740000 | Dronpa 145N ORF (E. coli optimized) | Green | 672 | In stock | ||||||
| BBa_K1615089 | mRFP RFC25 | Red | 675 | In stock | 11 | |||||
| BBa_K1742002 | Dronpa 145N | Green | 693 | In stock | 3 | |||||
| BBa_K1789003 | GFP1(with termination codon) | Green | 474 | In stock | 12 | |||||
| BBa_K1789004 | GFP2 | Green | 249 | In stock | 7 | |||||
| BBa_K1680006 | Fluorescent protein dronpa | Green | 669 | In stock | ||||||
| BBa_K1673010 | Partially UV-Resistant RFP | Red | 678 | In stock | 1 | |||||
| BBa_K1673101 | UV-Resistant RFP | Red | 678 | In stock | 2 | |||||
| BBa_K1673102 | UV Mutation Optimized RFP (Improved Expression) | Red | 681 | In stock | 1 | |||||
| BBa_K1673103 | UV Mutation Maximizied RFP | Red | 678 | In stock | 1 | |||||
| BBa_K106000 | GFP, AarI BD part | Forward | Green | 714 | It's complicated | |||||
| BBa_K106004 | mCherry, Aar1 AB part | Red | 708 | It's complicated | ||||||
| BBa_K106005 | mCherry, Aar1 BD part | Red | 708 | It's complicated | ||||||
| BBa_K106028 | GFP, AarI AB part | Green | 714 | It's complicated | ||||||
| BBa_K125810 | slr2016 signal sequence + GFP fusion for secretion of GFP | Forward | Green | 779 | It's complicated | |||||
| BBa_K106671 | GFP, Aar1 AD part | Green | 714 | It's complicated | 7 | |||||
| BBa_K180008 | mCherry | mCherry (rights owned by Clontech) | forward | Red | 708 | It's complicated | 6 | |||
| BBa_K1740001 | Dronpa145K ORF (E. coli optimized) | Green | 672 | It's complicated | ||||||
| BBa_K1742003 | Dronpa 145K | Green | 741 | It's complicated | 3 | |||||
| BBa_K1819000 | GFPmut3b | GFP coding sequence with an N-terminal linker | Forward | Green | 511 | 501 | 750 | It's complicated | 1 | |
| BBa_K1602021 | Green fluorescent protein (GFP) with N-terminal His-Tag | Green | 738 | It's complicated | 2 | |||||
| BBa_K1602024 | HisTag-GFP_Si4-Tag fusion protein | Green | 807 | It's complicated | 2 | |||||
| BBa_K1857000 | mVenus | Yellow | 1236 | It's complicated | ||||||
| BBa_K1680017 | Cre-Dronpa Fusion | Green | 1728 | It's complicated | ||||||
| BBa_K1680018 | Cre-Dronpa fusion | Green | 1746 | It's complicated | ||||||
| BBa_K1680019 | Cre-dronpa fusion | Green | 1764 | It's complicated | ||||||
| BBa_K1680020 | Cre-dronpa fusion | Green | 1782 | It's complicated | ||||||
| BBa_K1680021 | Dronpa caged Cre with NLS | Green | 2433 | It's complicated | ||||||
| BBa_K1680022 | Dronpa caged Cre with NLS | Green | 2451 | It's complicated | ||||||
| BBa_K1680023 | Dronpa caged Cre with NLS | Green | 2469 | It's complicated | ||||||
| BBa_K1680024 | Dronpa caged Cre with NLS | Green | 2487 | It's complicated | ||||||
| BBa_K1673104 | Oxidation-Resistant RFP | Red | 678 | It's complicated | 2 | |||||
| BBa_K1673410 | GFP (Mutation Optimized) | Green | 717 | It's complicated | ||||||
| BBa_K2003010 | Inducible Green Fluorescent Protein UnaG+6xHis-tag | 462 | It's complicated | |||||||
| BBa_K2455002 | SYFP2 | CPP-SYFP2 | C-terminal hexa-histidine | Forward | Yellow | 527 | 515 | 804 | It's complicated | |
| BBa_K2455005 | mTag BFP | CPP-mTag BFP | C-terminal hexa-histidine | Forward | Blue | 456 | 399 | 786 | It's complicated | |
| BBa_K2656013 | sfGFP Coding Sequence | 721 | It's complicated | 5 | ||||||
| BBa_K2656023 | GFPmut3b_LVA Coding Sequence | 754 | It's complicated | 1 | ||||||
| BBa_K2656014 | mRFP1 Coding Sequence | 679 | It's complicated | 3 | ||||||
| BBa_K2656024 | mRFP1_LVA Coding Sequence | 715 | It's complicated | 1 | ||||||
| BBa_K2656020 | YFP | YFP_LVA Coding Sequence | LVA | Forward | Yellow | 529 | 516 | 754 | It's complicated | 1 |
| BBa_K2656021 | YFP | YFP Coding Sequence | None | Forward | Yellow | 529 | 516 | 721 | It's complicated | 1 |
| BBa_K2558005 | sfGFP | superfold GFP (+LVA_ | Green | 758 | It's complicated | 6 | ||||
| BBa_K3781002 | mCerulean, MocloMania B3_B4 | 714 | -1 | |||||||
| BBa_K3781003 | mVenus, MoCloMania B3_B4 | 714 | -1 | |||||||
| BBa_K3781015 | mVenus, MocloMania B5 | 717 | -1 | |||||||
| BBa_K3781016 | mCerulean, MocloMania B5 | 717 | -1 | |||||||
| BBa_K3781012 | mCerulean, MocloMania B4 | 714 | -1 | |||||||
| BBa_K3831029 | Green fluorescent protein | 714 | -1 | |||||||
| BBa_K3831016 | sfGFP | 714 | -1 | |||||||
| BBa_K3831021 | mScarlet-I | 696 | -1 | |||||||
| BBa_K3831042 | GFP | 714 | -1 | |||||||
| BBa_K3765010 | sfGFP (Superfolder GFP) | 714 | -1 | |||||||
| BBa_K4139013 | PbEL04 | CAPE-GFP | His-Tag | Forward | 912 | -1 | ||||
| BBa_K4139015 | PbEL04 | COAPE - GFP | OmpA & His-Tag | Forward | 984 | -1 | ||||
| BBa_K4139017 | CAP-FP | CAP-FP | His-Tag | Forward | 1101 | -1 | ||||
| BBa_K4706008 | Green fluorescent GFP-like protein | eechGFP1 codon optimised S.cerevisae | none | forward | 497nm | 687 | -1 | |||
| BBa_K4759012 | sfGFP | 702 | -1 | |||||||
| BBa_K192001 | CFP +tgt +lva | 858 | Not in stock | |||||||
| BBa_K180001 | GFPmut3b | Green fluorescent protein (+LVA) | LVA | forward | Green | 754 | Not in stock | 1 | ||
| BBa_K180009 | mBanana | mBanana (rights owned by Clontech) | forward | Yellow | 708 | Not in stock | 3 | |||
| BBa_K863121 | GFPmut3b | green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag) | His6 | Green | 750 | Not in stock | ||||
| BBa_K863120 | GFPmut3b | green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard) | None | Green | 729 | Not in stock | ||||
| BBa_K1491001 | mTagBFP-His | Blue | 723 | Not in stock | 1 | |||||
| BBa_K1491004 | SuperYFP2-His | Yellow | 741 | Not in stock | 3 | |||||
| BBa_K1486006 | IFP[1] | Forward | Infrared | 405 | Not in stock | 6 | ||||
| BBa_K1486007 | IFP[2] | Forward | Infrared | 585 | Not in stock | 6 | ||||
| BBa_K1362461 | mRFP1 | **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) with barcode | None | Forward | Red | 607 | 584 | 706 | Not in stock | 3 |
| BBa_K1583017 | Blue Fluorescent Protein (mTagBFP) without stopcodon | Blue | 699 | Not in stock | 1 | |||||
| BBa_K1641224 | mCherry-ssrA | Red | 747 | Not in stock | 2 | |||||
| BBa_K1688019 | dTomato, red fluorescent protein | Red | 708 | Not in stock | 1 | |||||
| BBa_K1732020 | GFP-HIS | Green | 732 | Not in stock | 1 | |||||
| BBa_K1732021 | BFPCO-His | Blue | 717 | Not in stock | 1 | |||||
| BBa_K1732022 | EGFPCO-His | Green | 735 | Not in stock | 1 | |||||
| BBa_K1732024 | YFPCO-His | Yellow | 735 | Not in stock | 1 | |||||
| BBa_K1732025 | RFPCO-His | Red | 690 | Not in stock | 2 | |||||
| BBa_K1616016 | YC155 - C terminal YFP split | Yellow | 255 | Not in stock | 2 | |||||
| BBa_K1616017 | YN155 - N terminal YFP split | Yellow | 468 | Not in stock | 2 | |||||
| BBa_K1650038 | GFP-His | Green | 1159 | Not in stock | 1 | |||||
| BBa_K1799026 | Discosoma RFP with stp | Red | 675 | Not in stock | 2 | |||||
| BBa_K2040122 | KillerRed + NLS | Red | 744 | Not in stock | ||||||
| BBa_K3128008 | mRFP1 | Engineered mutant of red fluorescent protein with RFC21 restriction sites | None | Forward | Red | 607 | 584 | 690 | Not in stock | 1 |
| BBa_K2929004 | mOrange | mOrange + SpyTag | None | Orange | 562 | 548 | 829 | Not in stock | ||
| BBa_K3013003 | mamC-FbFP BS2 fusion | 819 | Not in stock | |||||||
| BBa_K2984019 | L1c-Psad-YFP-RbcS2, Expression of YFP through light-inducible promoter | 1805 | Not in stock | |||||||
| BBa_K3427000 | Heat-stable fluorescent protein YFP_LOV | 342 | Not in stock | -1 |
Luciferases
| Name | Protein | Description | Direction | Uniprot | KEGG | E.C. | Substrate | Product | Length | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| BBa_J52011 | dnMyD88-linker-Rluc | 1371 | In stock | |||||||
| BBa_I712019 | Firefly luciferase - luciferase from Photinus pyralis | 1653 | In stock | |||||||
| BBa_K322237 | Firefly luciferase from Photinus pyralis | 1653 | In stock | |||||||
| BBa_K1159001 | NanoLuc Luciferase in RFC[25] | 510 | In stock | |||||||
| BBa_K1761002 | Outer Membrane Protein X (OmpX) with BsoBI-linker and NanoLuc | 1241 | In stock | |||||||
| BBa_K1689001 | Coding sequence of STV-N-luc | 1727 | In stock | |||||||
| BBa_K1689006 | Coding sequence of FKBP-Cluc394 | 875 | In stock | |||||||
| BBa_K1680009 | NanoLuc Luciferase | 516 | In stock | |||||||
| BBa_K1680010 | Firefly Luciferase | 1653 | In stock | |||||||
| BBa_K1734004 | Nanoluc (Codon optimized for Sf9 cells) | 525 | In stock | |||||||
| BBa_K1616025 | NanoLuc | 510 | In stock | |||||||
| BBa_J52013 | dnMyD88-linker-Rluc-linker-PEST191 | 1872 | It's complicated | |||||||
| BBa_K525311 | Fusion Protein of S-Layer SgsE and Firefly-Luciferase | 4062 | It's complicated | |||||||
| BBa_K785003 | Lux(CDABEG) coding gene PoPS->Light | 6427 | It's complicated | |||||||
| BBa_K1486054 | CheY/CheZ + split rLuc | Forward | 3293 | It's complicated | ||||||
| BBa_K1486055 | CheY/CheZ + split fLuc | Forward | 4079 | It's complicated | ||||||
| BBa_K1732003 | GLUCCO-His | 522 | It's complicated | |||||||
| BBa_K1689004 | Coding sequence of Nluc398-FRB | 1553 | It's complicated | |||||||
| BBa_K1732027 | GLuc-His | 522 | It's complicated | |||||||
| BBa_K2023009 | Gaussia Luciferase | Gaussia luciferase coding sequence optimized for use in E.coli DH5-Alpha | 561 | It's complicated | ||||||
| BBa_K2637010 | Nanoluc+PEST | Forward | 642 | It's complicated | ||||||
| BBa_K284999 | Deletar este tambm | 1431 | Not in stock | |||||||
| BBa_K525411 | Fusion Protein of S-Layer SbpA and Firefly-Luciferase | 4854 | Not in stock | |||||||
| BBa_K1761005 | LargeBit Split Luciferase | 477 | Not in stock | |||||||
| BBa_K1761006 | SmallBit Split Luciferase | 36 | Not in stock | |||||||
| BBa_K1732029 | FireflyCO-His | 1668 | Not in stock |
Enzymes that produce colored substrates
| Name | Protein | Description | Direction | UniProt | KEGG | E.C. | Substrate | Product | Color | Length | Status |
|---|---|---|---|---|---|---|---|---|---|---|---|
| BBa_I732006 | lacZ alpha fragment | 234 | In stock | ||||||||
| BBa_I732005 | lacZ (encoding beta-galactosidase, full-length) | 3075 | It's complicated | ||||||||
| BBa_K147002 | xylE | 924 | It's complicated |
Miscellaneous
| Name | Protein | Description | Tag | Direction | UniProt | KEGG | Length | Status |
|---|---|---|---|---|---|---|---|---|
| BBa_K592009 | amilCP, blue chromoprotein | 669 | In stock | |||||
| BBa_K592010 | amilGFP, yellow chromoprotein | 699 | In stock | |||||
| BBa_K592011 | cjBlue, green chromoprotein | 702 | In stock | |||||
| BBa_K592012 | eforRed, red chromoprotein | 681 | In stock | |||||
| BBa_K864401 | aeBlue blue chromoprotein | 699 | In stock | |||||
| BBa_K1122007 | AmilCP (BBa_K592009) compatible with Genabler assembly | 685 | In stock | |||||
| BBa_K1033906 | tsPurple, purple chromoprotein | 690 | In stock | |||||
| BBa_K1033919 | gfasPurple, purple chromoprotein | 669 | In stock | |||||
| BBa_K1023005 | eforRed | 687 | In stock | |||||
| BBa_K1033932 | spisPink, pink chromoprotein | 678 | In stock | |||||
| BBa_K1824017 | AeBlue blue chromoprotein | 699 | In stock | |||||
| BBa_K678035 | yA (green pigment) from Aspergillus nidulans | 2156 | It's complicated | |||||
| BBa_K732001 | Synthetic Chorionic Gonadotropin, beta (synCG-β) | 498 | It's complicated | |||||
| BBa_K1824019 | AeBlue blue chromoprotein with aav tag | 738 | It's complicated | |||||
| BBa_K2656018 | amilCP Coding Sequence | 670 | It's complicated | |||||
| BBa_K4139011 | Truncated PbEL04 | Truncated PbEL04 | Thioredoxin & His-Tag | Forward | 963 | |||
| BBa_K4139012 | PbEL04 | Full Length PbEL04 | His-Tag | Forward | 570 | |||
| BBa_K4139018 | CAPE-AFP | CAPE-AFP | His-Tag | Forward | 537 | |||
| BBa_K4139019 | CP-MAF | Rec. CP-MAF | His-Tag | Forward | 495 | |||
| BBa_K4139020 | Pro1 | Pro1 | His-Tag | Forward | 1302 | |||
| BBa_K5466031 | AntiAFB1-scFv1 EpoR Split-N-mCerulean | 1809 | ||||||
| BBa_K5466032 | AntiAFB1-scFv2 EpoR Split-C-mCerulean | 1542 | ||||||
| BBa_K5527001 | amilCP purple, Purple chromoprotein | 669 | ||||||
| BBa_K5527002 | amilCP yellow, Yellow chromoprotein | 669 | ||||||
| BBa_K5527003 | amilCP light purple, Light purple chromoprotein | 669 | ||||||
| BBa_K1685005 | aeBlue | 699 | Not in stock | |||||
| BBa_K1685007 | aeBlue with LAA tag | 732 | Not in stock | |||||
| BBa_K1685008 | aeBlue with AAV tag | 732 | Not in stock | |||||
| BBa_K3697002 | manP expression cassette | 2142 | Not in stock |