Part:BBa_K4139013

CAPE-GFP

This construct starts with enhanced green fluorescent protein (eGFP) followed by our chimeric anti-PbEL04 (CAPE) which is a complementary to PbEL04. We hypothesized that CAPE was complementary to PbEL04 through simulations between the two proteins. Finally we added a His-tag to help with the purification of the protein. The reason for using eGFP is to show if proper binding has occurred. If proper binding has occurred then the protein will glow green when blue light in the 470 nm to 498 nm range.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 874
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 874
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 874
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 874
  
• 1000
  COMPATIBLE WITH RFC[1000]