Designed by: Dong-Jiunn Jeffery TRUONG Group: iGEM13_TU-Munich (2013-09-14)
Enhanced Cyan Fluorescent Protein (eCFP) in RFC
This Biobricks is a improved version BBa_E0020 which codes for the Enhanced Cyan Fluorescent Protein. We improve the function of the biobrick, to allow protein fusions which is originally not possible due to RFC pre- and suffix. This part is flanked by RFC pre- and suffix for further protein fusions.
Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein) ATGGCCGGCGTGAGCAAG ... CTGTACAAGACCGGT ORF from nucleotide position -8 to 720 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)