Firefly luciferase from Photinus pyralis In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light
This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.
Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal SapI.rc site found at 808