Dronpa caged Cre with NLS
This part contains the protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa part:BBa_K1680006 using a 6 amino acid Ser/Gly-linker part:BBa_K1680000 and the scar (Ser/Thr) as spacer. The construct has an N-terminal NLS sequence part:BBa_K1680004. In the expressed construct the two Dronpa domains should form multimers leading to an inactivated Cre recombinase that is reversibly activatable by Dronpa illumination (see Zhou et al 2012).
This Part is cloned and shipped in the pTUM104 (Part:BBa_K801004) and thereby galactose inducible in yeast.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 298
Illegal BglII site found at 2032
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 1154
Illegal SapI site found at 704
Illegal SapI site found at 1751