
Coding
Part:BBa_K2656023
Designed by: Adrián Requena Gutiérrez Group: iGEM18_Valencia_UPV (2018-09-22)
GFPmut3b_LVA Coding Sequence
Part BBa_K2656013 is the GFPmut3b coding sequence with the LVA degradation tag. It is the BBa_K1399004 original sequence adapted to be Golden Gate compatible. Thus, it can be used with Biobrick and Golden Braid assembly methods. The LVA tag causes an increase in protease activity and, therefore, a faster degradation of the reporter protein in the cell.
It can be combined with other compatible parts from our Valencia UPV IGEM 2018 Printeria Collection to assemble transcriptional units with a BsaI one-step reaction with the Golden Gate assembly protocol .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter/gfp
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
None |