Part:BBa_K863121

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)

GFP from Repressilator reporter in Standard 25 (Freiburg) See the untagged version for source information.

Usage and Biology

GFP (mut3b) [note that this part does not have a barcode]

Tagged version of gfp from Repressilator reporter. See the design page for more source information.

Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

• Excitation max - 501nm
• Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 4
  Illegal AgeI site found at 742
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 653