Difference between revisions of "Biosafety"

Revision as of 18:12, 26 September 2012

Semantic Containment

Here are parts that would add a semantic containment to genes, or parts that would allow the cell to read semantic containments gene.

*More...*
Name	Type	Description	Length
BBa_K1479017	Protein_Domain	just for test	100
BBa_K1479018	Protein_Domain	Just for test
BBa_K2086001	Coding	Serine Repeat Antigen (SerA)	1233
BBa_K914000	RNA	pLac-supD-T	241
BBa_K914009	Translational_Unit	P1003* Ser133->Amber Codon	967
BBa_K914011	Intermediate	supD-T - intermediate	178
BBa_K914012	Plasmid_Backbone	pSB1A2 with 1 amber mutation	2079
BBa_K914018	Translational_Unit	P1003** Kan resistant gene with 2 Amber Codon	967

Kill switch

Here are parts that could trigger cell death through disruption at the level of the protein and the cell.

*More...*
Name	Type	Description	Length
BBa_K1074011	Composite	PsdpRI+RBS+SDP	2207
BBa_K117000	Coding	Lysis gene (promotes lysis in colicin-producing bacteria strain)	144
BBa_K1172901	Coding	Alanine racemase from ''E. coli''	1080
BBa_K1172902	Device	alanine racemase (''alr'') under the control of the P_''tac'' promoter	1134
BBa_K1172908	Device	Part 1 of the Biosafety-System Lac of Growth (pRha lacI alr)	2509
BBa_K1172909	Device	Biosafety-System araCtive (AraC)	3281
BBa_K1172911	Device	Biosafety-System Lac of Growth (lacI)	3600
BBa_K1172912	Device	Part 1 of the Biosystem TetOR alive (pRha TetR alr)	2042
BBa_K1184000	Coding	KillerRed	723
BBa_K1197007	Composite	IPTG dependent B.subtilis Kill Switch construct	2006
BBa_K1223001	Project	P.A.S.E. 1 cassette	2148
BBa_K1223002	Project	P.A.S.E. 2 cassette	1210
BBa_K1405008	Composite	A Kill Switch with "memory" time repressed by IPTG	2926
BBa_K1616005	Coding	Holin/endolysin reversed - RBS and double T7 terminator	1379
BBa_K1616013	Coding	ccdB reversed - RBS and double T7 terminator	575
BBa_K1639022	Composite	pCons-TetR-pTet-TevProtease Suicide Switch	1546
BBa_K1767001	Composite	P(Lac)IQ RBS Aiia RBS LuxR term term luxpR	1818
BBa_K1767002	Composite	P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter	2666
BBa_K1767003	Composite	P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter	3597
BBa_K1767004	Composite	P(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter	1892
BBa_K1819008	Device	Promoter test circuit to analyze the kill switch efficiency	2725
BBa_K1820024	Generator	Phage inducible promoter 1997 +RBS+FspI + term	1160
BBa_K1820025	Generator	p2001-native RBS-Fsp1-B0015	1209
BBa_K1914000	Coding	E. coli Codon Optimised KillerOrange(EOKO)	717
BBa_K1914001	Device	pT7- E. coli optimised - KillerOrange (EOKO)	926
BBa_K1914003	Device	pT7- E. coli optimised - KillerRed (EOKR)	929
BBa_K1919100	Device	The device consist of temperature promotor and hokD coding gene.	1112
BBa_K2040122	Coding	KillerRed + NLS	744
BBa_K2086002	Composite	PyeaR - SerA	1499
BBa_K2150103	Coding	1204 toxin CDS	507
BBa_K2150104	Coding	6249 toxin CDS	319
BBa_K2232025	Composite	mazEF switch	1006
BBa_K2292002	Coding	Toxin-antitoxin mazEF	584
BBa_K2292006	Composite	MazEF kill switch	877
BBa_K2486026	Composite	pGadA-MazF kill switch	769
BBa_K2616002	Coding	Temperature Kill Switch	938
BBa_K2660006	Coding	N-Cas9	3459
BBa_K2660007	Coding	C-Cas9	648
BBa_K2757000	Composite	Art-175 fused with N-terminal DsbA 2-19 signal peptide and unidirectional pTet	1008
BBa_K2757001	Composite	Art-175 fused with N-terminal DsbA 2-19 signal peptide and bidirectional pTet	1065
BBa_K2817006	Composite	PcspA-RBS-mazF	535
BBa_K2896001	Coding	Thylakoid-bound SuperNova (for S. elongatus PCC 7942)	846
BBa_K2919000	DNA	ghoT toxin from E. coli K12	174
BBa_K302034	Translational_Unit	mazEF toxin-antitoxin cluster	607
BBa_K302035	Device	Sucrose-limitation induced kill switch	1006
BBa_K3036004	Composite	Cold triggered kill switch	649
BBa_K3036008	Coding	RelB with degradation-promoting tag RepA	1758
BBa_K3096052	RNA	CRISPR Array targeting the Plasmid DNA of the glucose dependent Exendin-4 expression system	147
BBa_K3096054	Composite	Cas3 Nuclease under the control of the AraC promoter pBAD	2803
BBa_K3096055	Composite	Type I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter	4487
BBa_K3190601	Composite	pGAL1-BI-I: BI-I CDS under inducible promoter	1181
BBa_K3215013	Composite	Tse2 Repressed by Arabinose Kill Switch	1950
BBa_K3215016	Composite	MazF repressed by IPTG Kill Switch	2886
BBa_K3237029	Composite	nucA	1754
BBa_K3273001	Coding	Serratia marcescens nucA nuclease	801
BBa_K3273008	Composite	Composite ADH2/TDH3 nucA	1849
BBa_K3273009	Composite	Composite HXT6 Bax	2039
BBa_K3273010	Composite	Composite MET 25 nucA	1113
BBa_K3317053	Coding	NucD	540
BBa_K3317059	Composite	NucE lysis construction	308
BBa_K3317060	Composite	NucD	572
BBa_K3317061	Composite	tetR repressed killswitch	1504
BBa_K3378002	Regulatory	Ferrous iron sensetive promoter fhuA1	55
BBa_K3512001	Coding	ccdB Toxin	306
BBa_K3588014	Composite	MazE-MazF Hypoxia induced kill switch	2211
BBa_K3634012	Composite	Glucose-Mediated Death Sensor	566
BBa_K3755043	RNA	Sok RNA	66
BBa_K3755045	Coding	Hok/Mok protein	217
BBa_K3755046	Composite	Double Regulated(trp+lac) Kill Switch	2352
BBa_K3848004	Composite	Lactate regulated kill switch	2837
BBa_K4133006	Coding	Repressor protein CtsR_Lp (Lactobacillus plantarum WCFS1) for Lactobacillus casei	468
BBa_K4133007	Regulatory	PclpC_Lp repressible promoter (Lactobacillus plantarum WCFS1)	59
BBa_K4133008	RBS	RBS_Lp (Lactobacillus plantarum WCFS1)	14
BBa_K4133009	Coding	Repressor protein CtsR_Lc (Lactococcus lactis) for Lactobacillus casei	456
BBa_K4133010	Regulatory	PclpC_Lc repressible promoter (Lactococcus lactis)	72
BBa_K4133011	RBS	RBS_Lc (Lactococcus lactis)	12
BBa_K4133012	Coding	LysKB317 (Endolysin) for Lactobacillus casei	894
BBa_K4202000	Coding	mazE(codon optimization for Bacillus subtilis)	249
BBa_K4202001	Coding	mazF(codon optimization for Bacillus subtilis)	421
BBa_K4202045	Generator	Sucrose regulated kill switch in Bacillus subtilis	1291
BBa_K4345020	Composite	5' UTR of hsp17 with ccdA fused to sfGFP	1197
BBa_K4345023	Composite	5' UTR of rpoH with ccdA fused to sfGFP	1395
BBa_K4345024	Composite	5' UTR of prfA with ccdA fused to sfGFP	1277
BBa_K515104	Composite	J23100 promoter - Antiholin	488
BBa_K515106	Composite	J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806	1973
BBa_K5480009	Composite	Y38-DpnI-TrrnB	2353
BBa_K628006	Composite	Protegrin-1 Kill Switch	353
BBa_K879917	Plasmid_Backbone	LacO ori	2072
BBa_K879918	Plasmid_Backbone	TetO ori	2074

XNase

Here are parts that could trigger cell death through disruption at the level of the DNA or RNA.

*More...*
Name	Type	Description	Length
BBa_K1172904	Coding	Rnase Ba (Barnase) from Bacillus amyloliquefaciens	339
BBa_K131000	Generator	ColE2 Operon	2640
BBa_K1479016	Promoter	-- No description --
BBa_K1479017	Protein_Domain	just for test	100
BBa_K150009	Generator	ColicinE1 Producer Controlled by 3OC6HSL Receiver Device	3180
BBa_K526001	Coding	ptrc*-Dpn	1552
BBa_K914001	Composite	pLac Colicin E2 Immunity protein	345
BBa_K914002	Intermediate	repressilator RBS Colicin E2 Immunity protein	282

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 ==Physical Containment==
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Difference between revisions of "Biosafety"

Revision as of 18:12, 26 September 2012

Contents

Semantic Containment

Kill switch

XNase

Physical Containment