Difference between revisions of "Biosafety"

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==More information about biosafety==
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* Quick links of pubmed research :
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[http://www.ncbi.nlm.nih.gov/pubmed?term=biosafety%20gmo biosafety gmo]
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[http://www.ncbi.nlm.nih.gov/pubmed?term=biosafety%20synthetic%20biology biosafety synthetic biology]
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[http://www.ncbi.nlm.nih.gov/pubmed?term=issue%20synthetic%20biology issue synthetic biology]
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We encourage you to do this kind of research as part of your bibliography for whatever research you are working on.
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* Here is the link a openwetware page that aims at being improve by the community. The idea is to improve biosafety practices in synthetic biology, through a report.
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[http://openwetware.org/wiki/How_safe_is_safe_enough:_towards_best_pratices_of_synthetic_biology How safe is safe enough?]
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==Semantic Containment==
 
==Semantic Containment==

Revision as of 08:42, 3 October 2012

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More information about biosafety

  • Quick links of pubmed research :

[http://www.ncbi.nlm.nih.gov/pubmed?term=biosafety%20gmo biosafety gmo]

[http://www.ncbi.nlm.nih.gov/pubmed?term=biosafety%20synthetic%20biology biosafety synthetic biology]

[http://www.ncbi.nlm.nih.gov/pubmed?term=issue%20synthetic%20biology issue synthetic biology]

We encourage you to do this kind of research as part of your bibliography for whatever research you are working on.

  • Here is the link a openwetware page that aims at being improve by the community. The idea is to improve biosafety practices in synthetic biology, through a report.

[http://openwetware.org/wiki/How_safe_is_safe_enough:_towards_best_pratices_of_synthetic_biology How safe is safe enough?]


Semantic Containment

Here are parts that would add a semantic containment to genes, or parts that would allow the cell to read semantic containments gene.


More...
NameTypeDescriptionLength
BBa_K1479017Protein_Domainjust for test100
BBa_K1479018Protein_DomainJust for test 
BBa_K2086001CodingSerine Repeat Antigen (SerA)1233
BBa_K914000RNApLac-supD-T241
BBa_K914009Translational_UnitP1003* Ser133->Amber Codon967
BBa_K914011IntermediatesupD-T - intermediate178
BBa_K914012Plasmid_BackbonepSB1A2 with 1 amber mutation2079
BBa_K914018Translational_UnitP1003** Kan resistant gene with 2 Amber Codon967


Kill switch

Here are parts that could trigger cell death through disruption at the level of the protein and the cell.


More...
NameTypeDescriptionLength
BBa_K1074011CompositePsdpRI+RBS+SDP2207
BBa_K117000CodingLysis gene (promotes lysis in colicin-producing bacteria strain)144
BBa_K1172901CodingAlanine racemase from ''E. coli''1080
BBa_K1172902Devicealanine racemase (''alr'') under the control of the P''tac'' promoter1134
BBa_K1172908DevicePart 1 of the Biosafety-System Lac of Growth (pRha lacI alr)2509
BBa_K1172909DeviceBiosafety-System araCtive (AraC)3281
BBa_K1172911DeviceBiosafety-System Lac of Growth (lacI)3600
BBa_K1172912DevicePart 1 of the Biosystem TetOR alive (pRha TetR alr)2042
BBa_K1184000CodingKillerRed723
BBa_K1197007CompositeIPTG dependent B.subtilis Kill Switch construct2006
BBa_K1223001ProjectP.A.S.E. 1 cassette2148
BBa_K1223002ProjectP.A.S.E. 2 cassette1210
BBa_K1405008CompositeA Kill Switch with "memory" time repressed by IPTG2926
BBa_K1616005CodingHolin/endolysin reversed - RBS and double T7 terminator1379
BBa_K1616013CodingccdB reversed - RBS and double T7 terminator575
BBa_K1639022CompositepCons-TetR-pTet-TevProtease Suicide Switch1546
BBa_K1767001CompositeP(Lac)IQ RBS Aiia RBS LuxR term term luxpR1818
BBa_K1767002CompositeP(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter2666
BBa_K1767003CompositeP(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter 3597
BBa_K1767004CompositeP(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter1892
BBa_K1819008DevicePromoter test circuit to analyze the kill switch efficiency2725
BBa_K1820024GeneratorPhage inducible promoter 1997 +RBS+FspI + term1160
BBa_K1820025Generatorp2001-native RBS-Fsp1-B00151209
BBa_K1914000CodingE. coli Codon Optimised KillerOrange(EOKO)717
BBa_K1914001DevicepT7- E. coli optimised - KillerOrange (EOKO)926
BBa_K1914003DevicepT7- E. coli optimised - KillerRed (EOKR)929
BBa_K1919100DeviceThe device consist of temperature promotor and hokD coding gene.1112
BBa_K2040122CodingKillerRed + NLS744
BBa_K2086002CompositePyeaR - SerA1499
BBa_K2150103Coding1204 toxin CDS507
BBa_K2150104Coding6249 toxin CDS319
BBa_K2232025CompositemazEF switch1006
BBa_K2292002CodingToxin-antitoxin mazEF584
BBa_K2292006CompositeMazEF kill switch 877
BBa_K2486026CompositepGadA-MazF kill switch 769
BBa_K2616002CodingTemperature Kill Switch938
BBa_K2660006CodingN-Cas93459
BBa_K2660007CodingC-Cas9648
BBa_K2757000CompositeArt-175 fused with N-terminal DsbA 2-19 signal peptide and unidirectional pTet1008
BBa_K2757001CompositeArt-175 fused with N-terminal DsbA 2-19 signal peptide and bidirectional pTet1065
BBa_K2817006CompositePcspA-RBS-mazF535
BBa_K2896001CodingThylakoid-bound SuperNova (for S. elongatus PCC 7942)846
BBa_K2919000DNAghoT toxin from E. coli K12174
BBa_K302034Translational_UnitmazEF toxin-antitoxin cluster607
BBa_K302035DeviceSucrose-limitation induced kill switch1006
BBa_K3036004CompositeCold triggered kill switch649
BBa_K3036008CodingRelB with degradation-promoting tag RepA1758
BBa_K3096052RNACRISPR Array targeting the Plasmid DNA of the glucose dependent Exendin-4 expression system147
BBa_K3096054CompositeCas3 Nuclease under the control of the AraC promoter pBAD2803
BBa_K3096055CompositeType I CRISPR/Cas system surveillance complex Cascade ABCDE under the control of the AraC promoter4487
BBa_K3190601CompositepGAL1-BI-I: BI-I CDS under inducible promoter1181
BBa_K3215013CompositeTse2 Repressed by Arabinose Kill Switch1950
BBa_K3215016CompositeMazF repressed by IPTG Kill Switch2886
BBa_K3237029CompositenucA1754
BBa_K3273001CodingSerratia marcescens nucA nuclease801
BBa_K3273008CompositeComposite ADH2/TDH3 nucA1849
BBa_K3273009CompositeComposite HXT6 Bax2039
BBa_K3273010CompositeComposite MET 25 nucA1113
BBa_K3317053CodingNucD540
BBa_K3317059CompositeNucE lysis construction308
BBa_K3317060CompositeNucD572
BBa_K3317061CompositetetR repressed killswitch1504
BBa_K3378002RegulatoryFerrous iron sensetive promoter fhuA155
BBa_K3512001CodingccdB Toxin306
BBa_K3588014CompositeMazE-MazF Hypoxia induced kill switch 2211
BBa_K3634012CompositeGlucose-Mediated Death Sensor566
BBa_K3755043RNASok RNA66
BBa_K3755045CodingHok/Mok protein217
BBa_K3755046CompositeDouble Regulated(trp+lac) Kill Switch2352
BBa_K3848004CompositeLactate regulated kill switch2837
BBa_K4133006CodingRepressor protein CtsR_Lp (Lactobacillus plantarum WCFS1) for Lactobacillus casei468
BBa_K4133007RegulatoryPclpC_Lp repressible promoter (Lactobacillus plantarum WCFS1)59
BBa_K4133008RBSRBS_Lp (Lactobacillus plantarum WCFS1)14
BBa_K4133009CodingRepressor protein CtsR_Lc (Lactococcus lactis) for Lactobacillus casei456
BBa_K4133010RegulatoryPclpC_Lc repressible promoter (Lactococcus lactis) 72
BBa_K4133011RBSRBS_Lc (Lactococcus lactis)12
BBa_K4133012CodingLysKB317 (Endolysin) for Lactobacillus casei894
BBa_K4202000CodingmazE(codon optimization for Bacillus subtilis)249
BBa_K4202001CodingmazF(codon optimization for Bacillus subtilis)421
BBa_K4202045GeneratorSucrose regulated kill switch in Bacillus subtilis1291
BBa_K4345020Composite5' UTR of hsp17 with ccdA fused to sfGFP1197
BBa_K4345023Composite5' UTR of rpoH with ccdA fused to sfGFP1395
BBa_K4345024Composite5' UTR of prfA with ccdA fused to sfGFP1277
BBa_K515104CompositeJ23100 promoter - Antiholin488
BBa_K515106CompositeJ23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K1128061973
BBa_K5480009CompositeY38-DpnI-TrrnB2353
BBa_K628006CompositeProtegrin-1 Kill Switch353
BBa_K879917Plasmid_BackboneLacO ori2072
BBa_K879918Plasmid_BackboneTetO ori2074


XNase

Here are parts that could trigger cell death through disruption at the level of the DNA or RNA.


More...
NameTypeDescriptionLength
BBa_K1172904CodingRnase Ba (Barnase) from Bacillus amyloliquefaciens339
BBa_K131000GeneratorColE2 Operon2640
BBa_K1479016Promoter -- No description --  
BBa_K1479017Protein_Domainjust for test100
BBa_K150009GeneratorColicinE1 Producer Controlled by 3OC6HSL Receiver Device3180
BBa_K526001Codingptrc*-Dpn1552
BBa_K914001CompositepLac Colicin E2 Immunity protein345
BBa_K914002Intermediaterepressilator RBS Colicin E2 Immunity protein282


Physical Containment