Difference between revisions of "Protein coding sequences/Reporters"
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Reporter protein coding sequences are used to measure gene expression.
 
Reporter protein coding sequences are used to measure gene expression.
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==Fluorescent proteins==
 
==Fluorescent proteins==
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<parttable>fluorescent_reporter_protein_coding_sequence</parttable>
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<!-- To include a part in this table, include the categories "//cds/reporter/fluorescentprotein" and set the part type to Coding under the Hard Information tab of the part. -->
  
 
==Luciferases==
 
==Luciferases==
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<parttable>luciferase_reporter_protein_coding_sequence</parttable>
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<!-- To include a part in this table, include the categories "//cds/reporter/luciferase" and set the part type to Coding under the Hard Information tab of the part. -->
  
 
==Enzymes that produce colored substrates==
 
==Enzymes that produce colored substrates==
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<parttable>color_reporter_protein_coding_sequence</parttable>
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<!-- To include a part in this table, include the categories "//cds/reporter/color" and "//cds/enzyme" and set the part type to Coding under the Hard Information tab of the part. -->
  
 
==Miscellaneous==
 
==Miscellaneous==
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<parttable>color_reporter_protein_coding_sequence</parttable>
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<!-- To include a part in this table, include the categories "//cds/reporter" and none of the above categories set the part type to Coding under the Hard Information tab of the part. -->

Revision as of 22:20, 17 January 2009

< Back to Protein coding sequences

Reporter protein coding sequences are used to measure gene expression.

Fluorescent proteins


More...
NameProteinDescriptionTagDirectionFluorescent ColorEmissionExcitationLengthStatusUses
BBa_E0030EYFPenhanced yellow fluorescent protein derived from A. victoria GFPNoneForwardYellow527514723In stock140
BBa_E0020ECFPengineered cyan fluorescent protein derived from A. victoria GFP NoneForwardCyan476439723In stock95
BBa_E1010mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)NoneForwardRed607584706In stock593
BBa_E0040GFPmut3b green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212NoneForwardGreen511501720In stock875
BBa_E2050mOrangederivative of mRFP1, yeast-optimizedNoneOrange562548769In stock15
BBa_J52021 dnTraf6-linker-GFPGreen  1446In stock1
BBa_J52026 dnMyD88-linker-GFPGreen  1155In stock1
BBa_I715022 Amino Portion of RFPRed  462In stock2
BBa_I715023 Carboxyl portion of RFPRed  220In stock1
BBa_I712028 CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequenceForwardRed  733In stock8
BBa_K125500 GFP fusion brickForwardGreen  718In stock13
BBa_K165005 Venus YFP, yeast optimized for fusionForwardYellow  744In stock2
BBa_K082003GFPGFP(+LVA)Green  756In stock31
BBa_K156009 OFP (orange fluorescent protein)ForwardOrange  864In stock1
BBa_K156010 SBFP2 (strongly enhanced blue fluorescent protein)Blue  720In stock9
BBa_K294055GFPmut3bGFP RFP HybridNoneGreen511501720In stock3
BBa_K283005 lpp_ompA_eGFP_streptavidin  1533In stock1
BBa_K157043 CFP  732In stock1
BBa_K411207 Green Fluorescent Protein with LVA tag (GFPLVA)Green  753In stock2
BBa_K648013 GFP with Standard 25 Prefix/Suffix Green  735In stock13
BBa_K525306 Fusion Protein of S-Layer SgsE and mCeruleanBlue  3129In stock 
BBa_K592100 Blue Fluorescent Protein (mTagBFP)Blue  705In stock41
BBa_K592101 Yellow Fluorescent Protein (YFP)Yellow  720In stock5
BBa_K864100 Super Yellow Fluorescent Protein 2 (SYFP2)Yellow  723In stock8
BBa_K1122005 dsRED (BBa_E1010) compatible with Genabler assemblyRed  697In stock 
BBa_K1122006 mCherry (BBa_J06504) compatible with Genabler assemblyRed  730In stock 
BBa_K1122008 mTagBFP (BBa_K592100) compatible with Genabler assemblyBlue  721In stock 
BBa_K1033916 amajLime, yellow-green chromoproteinYellow, Green  693In stock10
BBa_K1184000 KillerRedRed  723In stock5
BBa_K1159301 Super Yellow Fluorescent Protein 2 (SYFP2) in RFC[25]Yellow  714In stock1
BBa_K1159302 Enhanced Cyan Fluorescent Protein (eCFP) in RFC[25]Cyan  714In stock1
BBa_K1093002 sfCFPCyan  720In stock 
BBa_K1093003 sfBFP N-terminalBlue  645In stock 
BBa_K1093004 sfCFP N-terminalCyan  645In stock 
BBa_K1093017 mCherry C-terminalRed  243In stock 
BBa_K1399000mRFP1RFP from Discosoma striata (coral) with AAV-ssrA degradation tagSsrA-AAVForwardRed607584714In stock 
BBa_K1399001mRFP1RFP from Discosoma striata (coral) with LVA-ssrA degradation tagSsrA-LVA degradation tagForwardRed607584714In stock2
BBa_K1399002mRFP1RFP from Discosoma striata (coral) with LAA-ssrA degradation tag (wt)SsrA-LAA degradation tagForwardRed607584714In stock1
BBa_K1399003mRFP1RFP from Discosoma striata (coral) with DAS-ssrA degradation tagSsrA-DAS degradation tagForwardRed607584714In stock1
BBa_K1399005GFPmut3bGFP (mut3b) with AAV-ssrA degradation tagSsrA-AAV degradation tagForwardGreen511501753In stock4
BBa_K1399004GFPmut3bGFP (mut3b) with LVA-ssrA degradation tagSsrA-LVA degradation tagForwardGreen511501753In stock8
BBa_K1399006GFPmut3bGFP (mut3b) with LAA-ssrA degradation tagSsrA-LAA degradation tagForwardGreen511501753In stock2
BBa_K1399007GFPmut3bGFP (mut3b) with SsrA-DAS+2 degradation tagSsrA-DAS+2 degradation tagForwardGreen511501759In stock4
BBa_K1399008GFPmut3bGFP (mut3b) with DAS-ssrA degradation tagSsrA-DAS degradation tagForwardGreen511501753In stock2
BBa_K1351021 Monomeric Red Fluorescent Protein from Discosoma striataRed  675In stock 
BBa_K1323009 DsRed (RFP) Coding SequenceRed  678In stock4
BBa_K1323010 YFP Coding SequenceYellow  720In stock 
BBa_K1510017 red florescence protein RFC23Red  703In stock 
BBa_K1486056 CxpR & Split IFP1.4 [Cterm + Cterm][2]ForwardInfrared  3742In stock 
BBa_K1321337 sfGFP in Freiburg format (RFC 25) Green  711In stock27
BBa_K1418030 SYFP2Yellow  723In stock 
BBa_K1418040 CFPCyan  723In stock 
BBa_K1418041 CFP(ns)Cyan  717In stock 
BBa_K1740000 Dronpa 145N ORF (E. coli optimized)Green  672In stock 
BBa_K1615089 mRFP RFC25Red  675In stock11
BBa_K1742002 Dronpa 145NGreen  693In stock3
BBa_K1789003 GFP1(with termination codon)Green  474In stock12
BBa_K1789004 GFP2 Green  249In stock7
BBa_K1680006 Fluorescent protein dronpaGreen  669In stock 
BBa_K1673010 Partially UV-Resistant RFPRed  678In stock1
BBa_K1673101 UV-Resistant RFPRed  678In stock2
BBa_K1673102 UV Mutation Optimized RFP (Improved Expression)Red  681In stock1
BBa_K1673103 UV Mutation Maximizied RFPRed  678In stock1
BBa_K106000 GFP, AarI BD partForwardGreen  714It's complicated 
BBa_K106004 mCherry, Aar1 AB partRed  708It's complicated 
BBa_K106005 mCherry, Aar1 BD partRed  708It's complicated 
BBa_K106028 GFP, AarI AB partGreen  714It's complicated 
BBa_K125810 slr2016 signal sequence + GFP fusion for secretion of GFPForwardGreen  779It's complicated 
BBa_K106671 GFP, Aar1 AD partGreen  714It's complicated7
BBa_K180008mCherrymCherry (rights owned by Clontech)forwardRed  708It's complicated6
BBa_K1740001 Dronpa145K ORF (E. coli optimized) Green  672It's complicated 
BBa_K1742003 Dronpa 145KGreen  741It's complicated3
BBa_K1819000GFPmut3bGFP coding sequence with an N-terminal linkerForwardGreen511501750It's complicated1
BBa_K1602021 Green fluorescent protein (GFP) with N-terminal His-TagGreen  738It's complicated2
BBa_K1602024 HisTag-GFP_Si4-Tag fusion proteinGreen  807It's complicated2
BBa_K1857000 mVenusYellow  1236It's complicated 
BBa_K1680017 Cre-Dronpa FusionGreen  1728It's complicated 
BBa_K1680018 Cre-Dronpa fusionGreen  1746It's complicated 
BBa_K1680019 Cre-dronpa fusionGreen  1764It's complicated 
BBa_K1680020 Cre-dronpa fusionGreen  1782It's complicated 
BBa_K1680021 Dronpa caged Cre with NLSGreen  2433It's complicated 
BBa_K1680022 Dronpa caged Cre with NLSGreen  2451It's complicated 
BBa_K1680023 Dronpa caged Cre with NLSGreen  2469It's complicated 
BBa_K1680024 Dronpa caged Cre with NLSGreen  2487It's complicated 
BBa_K1673104 Oxidation-Resistant RFPRed  678It's complicated2
BBa_K1673410 GFP (Mutation Optimized)Green  717It's complicated 
BBa_K2003010 Inducible Green Fluorescent Protein UnaG+6xHis-tag  462It's complicated 
BBa_K2455002SYFP2CPP-SYFP2C-terminal hexa-histidineForwardYellow527515804It's complicated 
BBa_K2455005mTag BFPCPP-mTag BFPC-terminal hexa-histidineForwardBlue456399786It's complicated 
BBa_K2656013 sfGFP Coding Sequence  721It's complicated5
BBa_K2656023 GFPmut3b_LVA Coding Sequence  754It's complicated1
BBa_K2656014 mRFP1 Coding Sequence  679It's complicated3
BBa_K2656024 mRFP1_LVA Coding Sequence  715It's complicated1
BBa_K2656020YFPYFP_LVA Coding SequenceLVAForwardYellow529516754It's complicated1
BBa_K2656021YFPYFP Coding SequenceNoneForwardYellow529516721It's complicated1
BBa_K2558005sfGFPsuperfold GFP (+LVA_Green  758It's complicated6
BBa_K3781002 mCerulean, MocloMania B3_B4  714 -1
BBa_K3781003 mVenus, MoCloMania B3_B4  714 -1
BBa_K3781015 mVenus, MocloMania B5  717 -1
BBa_K3781016 mCerulean, MocloMania B5  717 -1
BBa_K3781012 mCerulean, MocloMania B4  714 -1
BBa_K3831029 Green fluorescent protein  714 -1
BBa_K3831016 sfGFP  714 -1
BBa_K3831021 mScarlet-I  696 -1
BBa_K3831042 GFP  714 -1
BBa_K3765010 sfGFP (Superfolder GFP)  714 -1
BBa_K4139013PbEL04CAPE-GFPHis-TagForward  912 -1
BBa_K4139015PbEL04COAPE - GFPOmpA & His-TagForward  984 -1
BBa_K4139017CAP-FPCAP-FPHis-TagForward  1101 -1
BBa_K4706008Green fluorescent GFP-like proteineechGFP1 codon optimised S.cerevisaenoneforward 497nm687 -1
BBa_K4759012 sfGFP  702 -1
BBa_K192001 CFP +tgt +lva  858Not in stock 
BBa_K180001GFPmut3bGreen fluorescent protein (+LVA)LVAforwardGreen  754Not in stock1
BBa_K180009mBananamBanana (rights owned by Clontech)forwardYellow  708Not in stock3
BBa_K863121GFPmut3bgreen fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)His6Green  750Not in stock 
BBa_K863120GFPmut3bgreen fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)NoneGreen  729Not in stock 
BBa_K1491001 mTagBFP-HisBlue  723Not in stock1
BBa_K1491004 SuperYFP2-HisYellow  741Not in stock3
BBa_K1486006 IFP[1]ForwardInfrared  405Not in stock6
BBa_K1486007 IFP[2]ForwardInfrared  585Not in stock6
BBa_K1362461mRFP1**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) with barcode NoneForwardRed607584706Not in stock3
BBa_K1583017 Blue Fluorescent Protein (mTagBFP) without stopcodonBlue  699Not in stock1
BBa_K1641224 mCherry-ssrARed  747Not in stock2
BBa_K1688019 dTomato, red fluorescent proteinRed  708Not in stock1
BBa_K1732020 GFP-HISGreen  732Not in stock1
BBa_K1732021 BFPCO-HisBlue  717Not in stock1
BBa_K1732022 EGFPCO-HisGreen  735Not in stock1
BBa_K1732024 YFPCO-HisYellow  735Not in stock1
BBa_K1732025 RFPCO-HisRed  690Not in stock2
BBa_K1616016 YC155 - C terminal YFP splitYellow  255Not in stock2
BBa_K1616017 YN155 - N terminal YFP splitYellow  468Not in stock2
BBa_K1650038 GFP-HisGreen  1159Not in stock1
BBa_K1799026 Discosoma RFP with stpRed  675Not in stock2
BBa_K2040122 KillerRed + NLSRed  744Not in stock 
BBa_K3128008mRFP1Engineered mutant of red fluorescent protein with RFC21 restriction sitesNoneForwardRed607584690Not in stock1
BBa_K2929004mOrangemOrange + SpyTag NoneOrange562548829Not in stock 
BBa_K3013003 mamC-FbFP BS2 fusion  819Not in stock 
BBa_K2984019 L1c-Psad-YFP-RbcS2, Expression of YFP through light-inducible promoter  1805Not in stock 
BBa_K3427000 Heat-stable fluorescent protein YFP_LOV  342Not in stock-1


Luciferases


More...
NameProteinDescriptionDirectionUniprotKEGGE.C.SubstrateProductLengthStatus
BBa_J52011 dnMyD88-linker-Rluc     1371In stock
BBa_I712019 Firefly luciferase - luciferase from Photinus pyralis     1653In stock
BBa_K322237 Firefly luciferase from Photinus pyralis     1653In stock
BBa_K1159001 NanoLuc Luciferase in RFC[25]     510In stock
BBa_K1761002 Outer Membrane Protein X (OmpX) with BsoBI-linker and NanoLuc     1241In stock
BBa_K1689001 Coding sequence of STV-N-luc     1727In stock
BBa_K1689006 Coding sequence of FKBP-Cluc394     875In stock
BBa_K1680009 NanoLuc Luciferase     516In stock
BBa_K1680010 Firefly Luciferase     1653In stock
BBa_K1734004 Nanoluc (Codon optimized for Sf9 cells)     525In stock
BBa_K1616025 NanoLuc      510In stock
BBa_J52013 dnMyD88-linker-Rluc-linker-PEST191     1872It's complicated
BBa_K525311 Fusion Protein of S-Layer SgsE and Firefly-Luciferase     4062It's complicated
BBa_K785003 Lux(CDABEG) coding gene PoPS->Light     6427It's complicated
BBa_K1486054 CheY/CheZ + split rLucForward     3293It's complicated
BBa_K1486055 CheY/CheZ + split fLucForward     4079It's complicated
BBa_K1732003 GLUCCO-His     522It's complicated
BBa_K1689004 Coding sequence of Nluc398-FRB      1553It's complicated
BBa_K1732027 GLuc-His     522It's complicated
BBa_K2023009Gaussia LuciferaseGaussia luciferase coding sequence optimized for use in E.coli DH5-Alpha     561It's complicated
BBa_K2637010 Nanoluc+PESTForward     642It's complicated
BBa_K284999 Deletar este tambm     1431Not in stock
BBa_K525411 Fusion Protein of S-Layer SbpA and Firefly-Luciferase     4854Not in stock
BBa_K1761005 LargeBit Split Luciferase     477Not in stock
BBa_K1761006 SmallBit Split Luciferase     36Not in stock
BBa_K1732029 FireflyCO-His     1668Not in stock


Enzymes that produce colored substrates


More...
NameProteinDescriptionDirectionUniProtKEGGE.C.SubstrateProductColorLengthStatus
BBa_I732006 lacZ alpha fragment     234In stock
BBa_I732005 lacZ (encoding beta-galactosidase, full-length)     3075It's complicated
BBa_K147002 xylE     924It's complicated


Miscellaneous


More...
NameProteinDescriptionDirectionUniProtKEGGE.C.SubstrateProductColorLengthStatus
BBa_I732006 lacZ alpha fragment     234In stock
BBa_I732005 lacZ (encoding beta-galactosidase, full-length)     3075It's complicated
BBa_K147002 xylE     924It's complicated