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iGEM Teams and Labs are using plant chassis in their synthetic biology projects. They have experimentally validated their parts, documented them on the Registry, and submitted samples to the Registry's repository.  
 
iGEM Teams and Labs are using plant chassis in their synthetic biology projects. They have experimentally validated their parts, documented them on the Registry, and submitted samples to the Registry's repository.  

Latest revision as of 15:52, 16 July 2020

iGEM Teams and Labs are using plant chassis in their synthetic biology projects. They have experimentally validated their parts, documented them on the Registry, and submitted samples to the Registry's repository.

The Registry does not currently have a preferred chassis, standard, or protocol(s), for plant synthetic biology. However, this collection aims to highlight parts meant to be used in the most common plant chassis by previous teams.


Previous iGEM Plant Projects

The following table contains iGEM teams that have worked in plant chassis, along with links to their project wiki and Registry parts. This is not an exhaustive list.

Team Year Parts Chassis Project Title
Valencia UPV 2016 Parts Nicotiana benthamiana HYPE-IT: Hack Your Plants Editing with Innovative Technologies
AHUT_China 2015 Parts Rehmannia glutinosa Nutrition Commander:A bio-device can control the APeGs of the plant metabolism
Cambridge-JIC 2015 Parts Marchantia polymorpha OpenScope - Open-source, 3D printable fluorescence microscope
CAU_China 2015 Parts Arabidopsis thaliana Herbicide vs Herbicide Resistant Gene
Georgia_State 2015 Parts Nicotiana tabacum Protein Products from Plants and Pichia: Novel Manufacturing of Analgesics and Cannabinoids
NRP-UEA-Norwich 2015 Parts Nicotiana benthamiana Engineering nutrition to increase colonic butryrate
NYMU-Taipei 2015 Parts Nicotiana tabacum Fight the Blight
UNIK_Copenhagen 2015 Parts Physcomitrella patens SpaceMoss: Using synthetic biology for space exploration
Valencia UPV 2015 Parts Nicotiana benthamiana AladDNA
Waterloo 2015 Parts Arabidopsis thaliana CRISPieR: re-engineering CRISPR-Cas9 with functional applications in eukaryotic systems
Cambridge-JIC 2014 Parts Marchantia polymorpha mösbi - the plant biosensor for everyone
Hannover 2014 Parts Arabidopsis thaliana, Nicotiana tabacum Plant against – Removing heavy metals from nature
NRP-UEA-Norwich 2014 Parts Nicotiana benthamiana Green Canary: Induction of chromoproteins to signal plant-pathogen interactions
Valencia UPV 2014 Parts Nicotiana benthamiana The Sexy Plant
TU-Munich 2013 Parts Physcomitrella patens PhyscoFilter – Clean different
Kyoto 2012 Parts - Flower Fairy E.coli
UEA-JIC_Norwich 2011 Parts Physcomitrella patens The evolution of synthetic biology; The introduction of new photosynthetic eukaryotes as model organisms.
Harvard 2010 Parts Arabidopsis thaliana iGarden: an Open Source Toolkit for Plant Engineering
Nevada 2010 Parts Nicotiana tabacum Development of Plant Biosensors for Environmental Monitoring Using Nicotiana tabacum Protoplasts as Transgenic Plant Models

Chassis

Nicotiana benthamiana

"N. benthamiana is a widely used experimental plant from the solanaceous group of flowering plants that includes tomatoes, potatoes and capsicums. It is widely used in plant pathology due to the large number of plant pathogens (viruses, bacteria, fungi, oomycetes etc) that can successfully infect it. Of importance to synthetic biologists, N. benthamiana, is easily genetically transformed, regenerated and amenable to facile methods for virus-induced gene silencing and transient protein expression..." - [http://2014.igem.org/Team:NRP-UEA-Norwich/Project_System NRP-UEA-Norwich 2014]


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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

  • The parts above have been tested or are intended to be used in Nicotiana benthamiana.
  • To add to this list, please use the category //chassis/eukaryote/nbenthamiana


iGEM Teams that have worked with Nicotiana benthamiana
  • [http://2016.igem.org/Team:Valencia_UPV Valencia UPV 2016 Wiki] | Parts
  • [http://2015.igem.org/Team:NRP-UEA-Norwich NRP-UEA-Norwich 2015 Wiki] | Parts
  • [http://2015.igem.org/Team:Valencia_UPV Valencia UPV 2015 Wiki] | Parts
  • [http://2014.igem.org/Team:NRP-UEA-Norwich NRP-UEA-Norwich 2014 Wiki] | Parts
  • [http://2014.igem.org/Team:Valencia_UPV Valencia UPV 2014 Wiki] | Parts

Marchantia polymorpha

"Marchantia polymorpha is [a] novel, eukaryotic multicellular chassis. Being a liverwort, it is one of the most primitive land plants around. Its small size and relative genetic simplicity make it easy to work with and an exciting new model organism in synthetic biology. Content to grow on agar plates, marchantia can be bioengineered in a standard lab with minimal extra equipment." - [http://2014.igem.org/Team:Cambridge-JIC Cambridge-JIC 2014]


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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

  • The parts above have been tested or are intended to be used in Marchantia polymorpha.
  • To add to this list, please use the category //chassis/eukaryote/mpolymorpha


iGEM Teams that have worked with Marchantia polymorpha
  • [http://2015.igem.org/Team:Cambridge-JIC Cambridge-JIC Wiki 2015] | Parts
  • [http://2014.igem.org/Team:Cambridge-JIC Cambridge-JIC Wiki 2014] | Parts

Arabidopsis thaliana

"Arabidopsis thaliana ... is easy to transform via R. radiobacter and has a short regeneration time." - [http://2014.igem.org/Team:Hannover Hannover 2014]

Status: 500 Content-type: text/html

Software error:

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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

  • The parts above have been tested or are intended to be used in Arabidopsis thaliana.
  • To add to this list, please use the category //chassis/eukaryote/athaliana


iGEM Teams that have worked with Arabidopsis thaliana
  • [http://2015.igem.org/Team:CAU_China CAU_China 2015] | Parts
  • [http://2015.igem.org/Team:Waterloo Waterloo 2015] | Parts
  • [http://2014.igem.org/Team:Hannover Hannover 2014] | Parts
  • [http://2010.igem.org/Team:Harvard Harvard 2010] | Parts


Physcomitrella patens

"The moss Physcomitrella patens belongs to the land plant division Bryophyta, which are one of the earliest representatives of the land plants (Embryophyta) having evolved from green algae about 470 million years ago during the early Paleozoic...The general organization of plant tissue into roots, stem and leaves is found in a much more basic version in mosses. They show a differentiated stem with simple leaves, usually only a single layer of cells thick and lacking veins, that are used to absorb water and nutrients. Instead of roots they have similar threadlike rhizoids. These have a primary function as mechanical attachment rather than extraction of soil nutrients.

However different mosses and vascular plants are because of the early diverge of the evolutionary lineages, they share fundamental genetic and physiological processes. Here researchers chose Physcomitrella patens as a model organism with a genome size of about 450 Mb along 27 chromosomes that is highly similar to other land plants in both exon-intron-structure and codon usage." - [http://2013.igem.org/Team:TU-Munich TU-Munich 2013]

Status: 500 Content-type: text/html

Software error:

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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

  • The parts above have been tested or are intended to be used in Physcomitrella patens.
  • To add to this list, please use the category //chassis/eukaryote/ppatens


iGEM Teams that have worked with Physcomitrella patens
  • [http://2015.igem.org/Team:UNIK_Copenhagen UNIK_Copenhagen 2015] | Parts
  • [http://2013.igem.org/Team:TU-Munich TU-Munich 2013] | Parts
  • [http://2011.igem.org/Team:UEA-JIC_Norwich UEA-JIC_Norwich 2011] | Parts


Nicotiana tabacum

Status: 500 Content-type: text/html

Software error:

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For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

  • The parts above have been tested or are intended to be used in Nicotiana tabacum.
  • To add to this list, please use the category //chassis/eukaryote/ntabacum


iGEM Teams that have worked with Nicotiana tabacum
  • [http://2015.igem.org/Team:Georgia_State Georgia_State 2015] | Parts
  • [http://2015.igem.org/Team:NYMU-Taipei NYMU-Taipei 2015] | Parts
  • [http://2014.igem.org/Team:Hannover Hannover 2014] | Parts
  • [http://2010.igem.org/Team:Nevada Nevada 2010] | Parts


Rhizobium radiobacter/Agrobacterium tumefaciens

Rhizobium radiobacter/Agrobacterium tumefaciens is used as a shuttle chassis to deliver constructs into different plant chassis. "[Rhizobium radiobacter] infects plants through its Ti plasmid. The Ti plasmid inserts a segment of its DNA, termed T-DNA, into the host organisms genome when the T-DNA is successfully integrated this causes the expression of the gene of interest by the plant. The T-DNA can be altered to include any gene construct that you would like, so it can be inserted into the host cells." - [http://2014.igem.org/Team:NRP-UEA-Norwich/Project_System NRP-UEA-Norwich 2014]


iGEM Teams that have worked with Rhizobium radiobacter/Agrobacterium tumefaciens
  • [http://2015.igem.org/Team:AHUT_China AHUT_China 2015] | Parts
  • [http://2015.igem.org/Team:CAU_China CAU_China 2015] | Parts
  • [http://2015.igem.org/Team:Georgia_State Georgia_State 2015] | Parts
  • [http://2015.igem.org/Team:NRP-UEA-Norwich NRP-UEA-Norwich 2015] | Parts
  • [http://2015.igem.org/Team:Valencia_UPV Valencia_UPV 2015] | Parts
  • [http://2015.igem.org/Team:Waterloo Waterloo 2015] | Parts
  • [http://2014.igem.org/Team:NRP-UEA-Norwich NRP-UEA-Norwich 2014] | Parts
  • [http://2014.igem.org/Team:Hannover Hannover 2014] | Parts
  • [http://2012.igem.org/Team:Toronto Toronto 2012]
  • [http://2012.igem.org/Team:Kyoto Kyoto 2012]
  • [http://2010.igem.org/Team:Harvard Harvard 2010]


Chlamydomonas reinhardtii

iGEM Teams that have worked with Chlamydomonas reinhardtii
  • [http://2011.igem.org/Team:UEA-JIC_Norwich UEA-JIC_Norwich 2011]
  • [http://2016.igem.org/Team:USP_UNIFESP-Brazil USP_UNIFESP-Brazil 2016]
  • [http://2016.igem.org/Team:Linkoping_Sweden Linkoping_Sweden 2016]
  • [http://2016.igem.org/Team:SZU-China SZU-China 2016]
  • [http://2016.igem.org/Team:FAFU-CHINA FAFU-CHINA 2016]
  • [http://2016.igem.org/Team:Cambridge-JIC Cambridge-JIC 2016]
  • Humboldt Berlin 2019

Other Resources

  • [http://openplant.org/ OpenPlant]
  • Golden Braid
  • [http://synbio.tsl.ac.uk/golden-gate/ SynBio @ TSL]