iGEM Teams and Labs are using plant chassis in their synthetic biology projects. They have experimentally validated their parts, documented them on the Registry, and submitted samples to the Registry's repository.

The Registry does not currently have a preferred chassis, standard, or protocol(s), for plant synthetic biology. However, this collection aims to highlight parts meant to be used in the most common plant chassis by previous teams.

Previous iGEM Plant Projects

The following table contains iGEM teams that have worked in plant chassis, along with links to their project wiki and Registry parts. This is not an exhaustive list.

Team Year Parts Chassis Project Title
Valencia UPV 2016 Parts Nicotiana benthamiana HYPE-IT: Hack Your Plants Editing with Innovative Technologies
AHUT_China 2015 Parts Rehmannia glutinosa Nutrition Commander:A bio-device can control the APeGs of the plant metabolism
Cambridge-JIC 2015 Parts Marchantia polymorpha OpenScope - Open-source, 3D printable fluorescence microscope
CAU_China 2015 Parts Arabidopsis thaliana Herbicide vs Herbicide Resistant Gene
Georgia_State 2015 Parts Nicotiana tabacum Protein Products from Plants and Pichia: Novel Manufacturing of Analgesics and Cannabinoids
NRP-UEA-Norwich 2015 Parts Nicotiana benthamiana Engineering nutrition to increase colonic butryrate
NYMU-Taipei 2015 Parts Nicotiana tabacum Fight the Blight
UNIK_Copenhagen 2015 Parts Physcomitrella patens SpaceMoss: Using synthetic biology for space exploration
Valencia UPV 2015 Parts Nicotiana benthamiana AladDNA
Waterloo 2015 Parts Arabidopsis thaliana CRISPieR: re-engineering CRISPR-Cas9 with functional applications in eukaryotic systems
Cambridge-JIC 2014 Parts Marchantia polymorpha mösbi - the plant biosensor for everyone
Hannover 2014 Parts Arabidopsis thaliana, Nicotiana tabacum Plant against – Removing heavy metals from nature
NRP-UEA-Norwich 2014 Parts Nicotiana benthamiana Green Canary: Induction of chromoproteins to signal plant-pathogen interactions
Valencia UPV 2014 Parts Nicotiana benthamiana The Sexy Plant
TU-Munich 2013 Parts Physcomitrella patens PhyscoFilter – Clean different
Kyoto 2012 Parts - Flower Fairy E.coli
UEA-JIC_Norwich 2011 Parts Physcomitrella patens The evolution of synthetic biology; The introduction of new photosynthetic eukaryotes as model organisms.
Harvard 2010 Parts Arabidopsis thaliana iGarden: an Open Source Toolkit for Plant Engineering
Nevada 2010 Parts Nicotiana tabacum Development of Plant Biosensors for Environmental Monitoring Using Nicotiana tabacum Protoplasts as Transgenic Plant Models


Nicotiana benthamiana

"N. benthamiana is a widely used experimental plant from the solanaceous group of flowering plants that includes tomatoes, potatoes and capsicums. It is widely used in plant pathology due to the large number of plant pathogens (viruses, bacteria, fungi, oomycetes etc) that can successfully infect it. Of importance to synthetic biologists, N. benthamiana, is easily genetically transformed, regenerated and amenable to facile methods for virus-induced gene silencing and transient protein expression..." - NRP-UEA-Norwich 2014

NameDescriptionLengthCreated byresistanceoriginType
BBa_K1467104GoldenGate compatible MAS promoter391Cara Deal  Regulatory
BBa_K1467101GoldenGate compatible 35s Promoter 1432Cara Deal  Regulatory
BBa_K1467102GoldenGate compatible BS3 promoter + 51032Cara Deal  Regulatory
BBa_K1467103GoldenGate compatible PDF1.2 promotor1050Cara Deal  Regulatory
BBa_K1467204GoldenGate compatible Green Flourescent Protein (GFP)730Cara Deal  Reporter
BBa_K1554000TA29 promoter859Valencia_UPV team members  Regulatory
BBa_K1554002HarFAR1365Valencia_UPV team members  Coding
BBa_K1554001AtrΔ11981Valencia_UPV team members  Coding
BBa_K1742008PhiC31 Reporter attP:T35S:attB:omegaUTR412Ruben Escriba Piera  Reporter
BBa_K1742002Dronpa 145N693Ivn Casas Rodrigo  Coding
BBa_K1742006BxBI Reporter (attB:T35S:attP:OmegaUTR)397Ivn Casas Rodrigo  Reporter
BBa_K1618028Chloroplast Transit Peptide177Leda Coelewij  Coding
BBa_K161803735s Terminator212Leda Coelewij  Terminator
BBa_K1467203GoldenGate compatible Bax - cell death inducer589Cara Deal  Reporter
BBa_K1467205AvrBS3 TAL Effector - Induces BS33505Cara Deal  Coding
BBa_K1554003EaDAcT1092Valencia_UPV team members  Coding
BBa_K1554004Yellow biosafety module for plants3394Valencia_UPV team members  Device
BBa_K1554005Blue biosafety module for plants3364Valencia_UPV team members  Device
BBa_K1742001Entertoxin LTB414Ruben Escriba Piera  Coding
BBa_K1742000Avena sativa LOV2 domain456Ruben Escriba Piera  Coding
BBa_K1742003Dronpa 145K741Ivn Casas Rodrigo  Coding
BBa_K1742004PhiC31 Plant codon optimized1836Ivn Casas Rodrigo  Coding
BBa_K1742005BxBI integrase1693Ivn Casas Rodrigo  Coding
BBa_K1618029Acyltransferase Candidate 1 -Fused to GFP -Expression Construct3134Leda Coelewij  Composite
BBa_K174201135S:LacIBDBD-DronpaK:T35S - 35S:DronpaN-VP16:T35S2592Mnica Victoria Gutirrez Salazar  Device
BBa_K174200935S:BxbI:T35S - 35S:ReporterBxBI::GFP:T35S2964Pilar Baldominos  Device
BBa_K174201035S:LexABD-DronpaK:T35S - 35S:DronpaN-VP16:T35S3176Pilar Baldominos  Device
BBa_K174201235S:Gal4BD-DronpaK:T35S - 35S:DronpaN-VP16:T35S3610Mnica Victoria Gutirrez Salazar  Device
BBa_K1618030Acyltransferase Candidate 2 -Fused to GFP -Expression Construct3407Leda Coelewij  Composite
BBa_K1618031Acyltransferase Candidate 3 -Fused to GFP -Expression Construct3485Leda Coelewij  Composite
BBa_K1618032Acyltransferase Candidate 4 -Fused to GFP -Expression Construct3659Leda Coelewij  Composite
BBa_K1618033Acyltransferase Candidate 1 Expression Construct2396Leda Coelewij  Composite
BBa_K1618034Acyltransferase Candidate 2 Expression Construct2669Leda Coelewij  Composite
BBa_K1618035Acyltransferase Candidate 3 Expression Construct2747Leda Coelewij  Composite
BBa_K1618036Acyltransferase Candidate 4 Expression Construct2921Leda Coelewij  Composite
BBa_K174201335S:PhiC31:T35S - 35S:ReporterPhiC31::GFP:T35S5508Ruben Escriba Piera  Device
BBa_K201701235s + TFL consense + RSIAT-Luciferase + Tnos2836Monica Victoria Gutierrez Salazar  Device
BBa_K2017000C-split Cas9 + DnaE C-intein2358Monica Victoria Gutierrez Salazar  Protein_Domain
BBa_K2017001N-split Cas9 + DnaE N-intein2241Monica Victoria Gutierrez Salazar  Protein_Domain
BBa_K201700735s:5'+ Ga20ox consense + SAGTI-Luciferase + Tnos3057Monica Victoria Gutierrez Salazar  Device
BBa_K201700835s + Ga20ox consense + RSIAT-Luciferase + Tnos2836Monica Victoria Gutierrez Salazar  Device
BBa_K201700935s + Ga20ox consense + AEK-Luciferase + Tnos2872Monica Victoria Gutierrez Salazar  Device
BBa_K201701135s:5' + TFL consense + SAGTI-Luciferase + Tnos3057Monica Victoria Gutierrez Salazar  Device
BBa_K2017010 35s + Ga20ox consense + RSIAT-TEV-Luciferase + Tnos2869Monica Victoria Gutierrez Salazar  Device
BBa_K2017014 35s + TFL consense + RSIAT-TEV-Luciferase + Tnos2869Monica Victoria Gutierrez Salazar  Device
BBa_K2017013 35s + TFL consense + AEK-Luciferase + Tnos2872Monica Victoria Gutierrez Salazar  Device
BBa_K226900935s: PhyB: VP16:NLS: Tnos3970Jos lvaro Ballesteros Gonzlez  Composite
BBa_K4213000Plant Thiamine Pyrophosphate Riboswitch675Ioannis Retalis  Regulatory
  • The parts above have been tested or are intended to be used in Nicotiana benthamiana.
  • To add to this list, please use the category //chassis/eukaryote/nbenthamiana

iGEM Teams that have worked with Nicotiana benthamiana

Marchantia polymorpha

"Marchantia polymorpha is [a] novel, eukaryotic multicellular chassis. Being a liverwort, it is one of the most primitive land plants around. Its small size and relative genetic simplicity make it easy to work with and an exciting new model organism in synthetic biology. Content to grow on agar plates, marchantia can be bioengineered in a standard lab with minimal extra equipment." - Cambridge-JIC 2014

NameDescriptionLengthCreated byresistanceoriginType
BBa_K1484215nopaline synthase terminator293Salil Bhate  Terminator
BBa_K1484000AsPink chromoprotein in GoldenGate standard721Virginie Rutten  Coding
BBa_K1484001tsPurple chromoprotein in GoldenGate standard709Virginie Rutten  Coding
BBa_K1484002AmajLime chromoprotein in BBa and MoClo standard711Virginia Rutten  Coding
BBa_K1484104N7 nuclear localisation tag262Salil Bhate  Tag
BBa_K1484106LTI membrane localisation tag238Salil Bhate  Coding
BBa_K1484214MpEF1a (Marchantia poylmorpha Elongation Factor 1, PlantSyntax Pro+5U)1756Salil Bhate  Regulatory
BBa_K1484316Mp Selection Cassette3559Salil Bhate  Temporary
  • The parts above have been tested or are intended to be used in Marchantia polymorpha.
  • To add to this list, please use the category //chassis/eukaryote/mpolymorpha

iGEM Teams that have worked with Marchantia polymorpha

Arabidopsis thaliana

"Arabidopsis thaliana ... is easy to transform via R. radiobacter and has a short regeneration time." - Hannover 2014

NameDescriptionLengthCreated byresistanceoriginType
BBa_K1478000Expa4 plant secretion signal, localizes to extracellular space60Fabian Frmling  Protein_Domain
BBa_K1478001Cellulose binding domain309Fabian Frmling  Protein_Domain
BBa_K382000pORE Open Series Vector with BioBrick MCS (Basta resistance)7208Christina Agapakis  Plasmid
BBa_K382001pORE Open Serices Vector with BioBrick MCS (Kan resistance)7451Christina Agapakis  Plasmid_Backbone
BBa_K382003pORE Expression Series Vector with BioBrick MCS (Kan reistance)7969Christina Agapakis  Plasmid
BBa_K382002pORE Expression Series Vector with BioBrick MCS, Basta resistance7786Mara C. Inniss  Plasmid
BBa_K382004pORE Gus Reporter Series Vector with BiOBrick MCS (Kan resistance)9295Christina Agapakis  Plasmid_Backbone
BBa_K382005pORE GFP Reporter Series Vector with BioBrick MCS (Kan resistance)8203Christina Agapakis  Plasmid_Backbone
BBa_K382010amiRNA Construct for lycopene epsilon cyclase (LUT2)413Patrick Boyle  RNA
BBa_K382012amiRNA Construct for lycopene beta cyclase (LYC)413Christina Agapakis  RNA
BBa_K382011amiRNA Construct for carotene beta-ring hydroxylase (BETA-OHASE 1)413Mara C. Inniss  RNA
BBa_K382014ihpRNA construct against Arabidopsis Bet v 1 allergen1288Christina Agapakis  RNA
BBa_K382020Arabidopsis optimized Brazzein165Patrick Boyle  Coding
BBa_K382021Arabidopsis optimized Miraculin660Patrick Boyle  Coding
BBa_K382015ihpRNA construct against Arabidopsis LTP Allergen1372Christina Agapakis  RNA
BBa_K382022pENTCUP2 plant specific promoter503Patrick Boyle  Regulatory
BBa_K382017ihpRNA construct against Arabidopsis LTP Allergen424Christina Agapakis  RNA
BBa_K382018PDK intron793Christina Agapakis  Intermediate
BBa_K382019ihpRNA construct against Arabidopsis Ger3 allergen1429Christina Agapakis  RNA
BBa_K382034Act2pLacO (Actin promoter + 2x LacO)1241Mara C. Inniss  Regulatory
BBa_K382025Arabidopsis optimized Miraculin with StrepII N-terminus tag696Patrick Boyle  Coding
BBa_K382026Arabidopsis optimized Brazzein with StrepII N-terminus201Patrick Boyle  Coding
BBa_K382040Arabidopsis optimized Miraculin+2xYFP2160Mara C. Inniss  Coding
BBa_K382041Arabidopsis optimized Brazzein+2xYFP1665Mara C. Inniss  Coding
BBa_K382027Arabidopsis optimized Miraculin with StrepII C-terminus tag696Patrick Boyle  Coding
BBa_K382028Arabidopsis optimized Brazzein with StrepII C-terminus201Patrick Boyle  Coding
BBa_K1478002Top metal binding protein. Binds zinc, arsenic, copper and cadmium.909Fabian Frmling  Coding
BBa_K1982002Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)1854Zexu Li  Coding
BBa_K1982003CIBN(the N-terminal fragment of CIB1)612Zexu Li  Coding
BBa_K1982010CRY2-VP64(Eukaryotic LACE system)2100Zexu Li  Coding
BBa_K1982009Eukaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)1848Zexu Li  Coding
BBa_K4213000Plant Thiamine Pyrophosphate Riboswitch675Ioannis Retalis  Regulatory
  • The parts above have been tested or are intended to be used in Arabidopsis thaliana.
  • To add to this list, please use the category //chassis/eukaryote/athaliana

iGEM Teams that have worked with Arabidopsis thaliana

Physcomitrella patens

"The moss Physcomitrella patens belongs to the land plant division Bryophyta, which are one of the earliest representatives of the land plants (Embryophyta) having evolved from green algae about 470 million years ago during the early Paleozoic...The general organization of plant tissue into roots, stem and leaves is found in a much more basic version in mosses. They show a differentiated stem with simple leaves, usually only a single layer of cells thick and lacking veins, that are used to absorb water and nutrients. Instead of roots they have similar threadlike rhizoids. These have a primary function as mechanical attachment rather than extraction of soil nutrients.

However different mosses and vascular plants are because of the early diverge of the evolutionary lineages, they share fundamental genetic and physiological processes. Here researchers chose Physcomitrella patens as a model organism with a genome size of about 450 Mb along 27 chromosomes that is highly similar to other land plants in both exon-intron-structure and codon usage." - TU-Munich 2013

NameDescriptionLengthCreated byresistanceoriginType
BBa_K1159010Secretory NanoLuc Luciferase (SERK-SigP_nLuc) in RFC[25] N-Part616TU Munich 2013  Coding
BBa_K1159000Erythromycin Esterase Type II (EreB) in RFC[25]1254Louise FUNKE  Coding
BBa_K1159001NanoLuc Luciferase in RFC[25]510TU Munich 2013  Coding
BBa_K1159003Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]522TU Munich 2013  Coding
BBa_K1159205Secretory SpyTag (SERK-SigP_SpyTag) in RFC[25] N-Part145Dong-Jiunn Jeffery TRUONG  Coding
BBa_K1159202Secretory SpyCatcher (IgKappa-SigP_SpyCatcher) in RFC[25] N-Part412Dong-Jiunn Jeffery TRUONG  Coding
BBa_K1159303Signal Peptide of SERK Receptor from Physcomitrella patens in RFC[25]100TU Munich 2013  Protein_Domain
BBa_K115930735S Terminator of Cauliflower Mosaic Virus (CaMV)217TU Munich 2013  Terminator
BBa_K1159304Signal Peptide of Ig Kappa chain from Mus musculus in RFC[25] N-part67TU Munich 2013  Protein_Domain
BBa_K1159305Transmembrane domain of SERK Receptor from Physcomitrella patens in RFC[25]186TU Munich 2013  Protein_Domain
BBa_K1159308Neomycin phosphotransferase II (nptII) plant expression cassette for G418 resistance1517Dong-Jiunn Jeffery TRUONG  Device
BBa_K1159006Secretory NanoLuc Luciferase (IgKappa-SigP_nLuc) in RFC[25] N-Part583TU Munich 2013  Coding
BBa_K1159315TMD of SERK Receptor from Physcomitrella patens with C-terminal GFP fusion in RFC[25]933TU Munich 2013  Coding
BBa_K1159316SERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]996TU Munich 2013  Coding
BBa_K1159314TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part292Dong-Jiunn Jeffery TRUONG  Coding
BBa_K1159120Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)6751Dong-Jiunn Jeffery TRUONG  Translational_Unit
BBa_K1159203Secretory SpyTag (IgKappa-SigP_SpyTag) in RFC[25] N-Part112Dong-Jiunn Jeffery TRUONG  Coding
BBa_K1159204Secretory SpyCatcher (SERK-SigP_SpyCatcher) in RFC[25] N-Part445Dong-Jiunn Jeffery TRUONG  Coding
BBa_K1159207Membrane-anchored SpyTag in RFC[25] N-Part1147Dong-Jiunn Jeffery TRUONG  Coding
BBa_K1159206Membrane-anchored SpyCatcher in RFC[25] N-Part1447Dong-Jiunn Jeffery TRUONG  Coding
BBa_K1825000UNIK antifreeze protein324Adam Petersen  Coding
BBa_K1825005nptII795Jonathan Asmund Arnesen  Coding
BBa_K1159015Membrane-anchored NanoLuc Luciferase in RFC[25] N-Part1585TU Munich 2013  Coding
BBa_K1159306Actin5 Promoter from Physcomitrella patens1948Ingmar Polte  Regulatory
BBa_K182500435s CaMV390Jonathan Asmund Arnesen  Regulatory
BBa_K182500635s CaMV + nptII1191Jonathan Asmund Arnesen  Composite
BBa_K1825008Stilbene synthase1170Jonathan Asmund Arnesen  Coding
  • The parts above have been tested or are intended to be used in Physcomitrella patens.
  • To add to this list, please use the category //chassis/eukaryote/ppatens

iGEM Teams that have worked with Physcomitrella patens

Nicotiana tabacum

There are no parts for this table

  • The parts above have been tested or are intended to be used in Nicotiana tabacum.
  • To add to this list, please use the category //chassis/eukaryote/ntabacum

iGEM Teams that have worked with Nicotiana tabacum

Rhizobium radiobacter/Agrobacterium tumefaciens

Rhizobium radiobacter/Agrobacterium tumefaciens is used as a shuttle chassis to deliver constructs into different plant chassis. "[Rhizobium radiobacter] infects plants through its Ti plasmid. The Ti plasmid inserts a segment of its DNA, termed T-DNA, into the host organisms genome when the T-DNA is successfully integrated this causes the expression of the gene of interest by the plant. The T-DNA can be altered to include any gene construct that you would like, so it can be inserted into the host cells." - NRP-UEA-Norwich 2014

iGEM Teams that have worked with Rhizobium radiobacter/Agrobacterium tumefaciens

Chlamydomonas reinhardtii

iGEM Teams that have worked with Chlamydomonas reinhardtii

Other Resources