Collections/Plants

iGEM Teams and Labs are using plant chassis in their synthetic biology projects. They have experimentally validated their parts, documented them on the Registry, and submitted samples to the Registry's repository.

The Registry does not currently have a preferred chassis, standard, or protocol(s), for plant synthetic biology. However, this collection aims to highlight parts meant to be used in the most common plant chassis by previous teams.


Previous iGEM Plant Projects

The following table contains iGEM teams that have worked in plant chassis, along with links to their project wiki and Registry parts. This is not an exhaustive list.

Team Year Parts Chassis Project Title
Valencia UPV 2016 Parts Nicotiana benthamiana HYPE-IT: Hack Your Plants Editing with Innovative Technologies
AHUT_China 2015 Parts Rehmannia glutinosa Nutrition Commander:A bio-device can control the APeGs of the plant metabolism
Cambridge-JIC 2015 Parts Marchantia polymorpha OpenScope - Open-source, 3D printable fluorescence microscope
CAU_China 2015 Parts Arabidopsis thaliana Herbicide vs Herbicide Resistant Gene
Georgia_State 2015 Parts Nicotiana tabacum Protein Products from Plants and Pichia: Novel Manufacturing of Analgesics and Cannabinoids
NRP-UEA-Norwich 2015 Parts Nicotiana benthamiana Engineering nutrition to increase colonic butryrate
NYMU-Taipei 2015 Parts Nicotiana tabacum Fight the Blight
UNIK_Copenhagen 2015 Parts Physcomitrella patens SpaceMoss: Using synthetic biology for space exploration
Valencia UPV 2015 Parts Nicotiana benthamiana AladDNA
Waterloo 2015 Parts Arabidopsis thaliana CRISPieR: re-engineering CRISPR-Cas9 with functional applications in eukaryotic systems
Cambridge-JIC 2014 Parts Marchantia polymorpha mösbi - the plant biosensor for everyone
Hannover 2014 Parts Arabidopsis thaliana, Nicotiana tabacum Plant against – Removing heavy metals from nature
NRP-UEA-Norwich 2014 Parts Nicotiana benthamiana Green Canary: Induction of chromoproteins to signal plant-pathogen interactions
Valencia UPV 2014 Parts Nicotiana benthamiana The Sexy Plant
TU-Munich 2013 Parts Physcomitrella patens PhyscoFilter – Clean different
Kyoto 2012 Parts - Flower Fairy E.coli
UEA-JIC_Norwich 2011 Parts Physcomitrella patens The evolution of synthetic biology; The introduction of new photosynthetic eukaryotes as model organisms.
Harvard 2010 Parts Arabidopsis thaliana iGarden: an Open Source Toolkit for Plant Engineering
Nevada 2010 Parts Nicotiana tabacum Development of Plant Biosensors for Environmental Monitoring Using Nicotiana tabacum Protoplasts as Transgenic Plant Models

Chassis

Nicotiana benthamiana

"N. benthamiana is a widely used experimental plant from the solanaceous group of flowering plants that includes tomatoes, potatoes and capsicums. It is widely used in plant pathology due to the large number of plant pathogens (viruses, bacteria, fungi, oomycetes etc) that can successfully infect it. Of importance to synthetic biologists, N. benthamiana, is easily genetically transformed, regenerated and amenable to facile methods for virus-induced gene silencing and transient protein expression..." - [http://2014.igem.org/Team:NRP-UEA-Norwich/Project_System NRP-UEA-Norwich 2014]



More...
NameDescriptionTypeCreated bylengthusesseq
BBa_K1467101GoldenGate compatible 35s Promoter RegulatoryCara Deal143210 . . . acaattacatttacaattatcgatacaatg
BBa_K1467102GoldenGate compatible BS3 promoter + 5RegulatoryCara Deal1032  . . . agtagtcctagttgcacatatatttcaatg
BBa_K1467103GoldenGate compatible PDF1.2 promotorRegulatoryCara Deal1050  . . . ttgaaaacaaaatagtaataatcatcaatg
BBa_K1467104GoldenGate compatible MAS promoterRegulatoryCara Deal391  . . . ataaccaatctcgatacaccaaatcgaatg
BBa_K1467204GoldenGate compatible Green Flourescent Protein (GFP)ReporterCara Deal7306 . . . atggacgagctgtacaagtaagcttaataa
BBa_K1554000TA29 promoterRegulatoryValencia_UPV team members859  . . . gtttttacttaaagaaattagctaaaaatg
BBa_K1554002HarFARCodingValencia_UPV team members1365  . . . aggcacttcttggaaaagaaatatcggtga
BBa_K1554001AtrΔ11CodingValencia_UPV team members981  . . . ggtactgatatgtggggtaggaaacgttga
BBa_K1742002Dronpa 145NCodingIvn Casas Rodrigo6933 . . . caggctaaacctaaaaagaagagaaaagtt
BBa_K1742006BxBI Reporter (attB:T35S:attP:OmegaUTR)ReporterIvn Casas Rodrigo397  . . . aacaacaaacaaaatacaaacaacaacaac
BBa_K1742008PhiC31 Reporter attP:T35S:attB:omegaUTRReporterRuben Escriba Piera412  . . . aacaacaaacaaaatacaaacaacaacaac
BBa_K1618028Chloroplast Transit PeptideCodingLeda Coelewij1778 . . . attgctagtaacggaggaagagttcgagca
BBa_K161803735s TerminatorTerminatorLeda Coelewij2128 . . . ccaaaatccagtactaaaatccagatcgct
BBa_K1467203GoldenGate compatible Bax - cell death inducerReporterCara Deal589  . . . atctggaagaagatgggttaggcttaataa
BBa_K1467205AvrBS3 TAL Effector - Induces BS3CodingCara Deal3505  . . . atggaacttctgcctcagtaggcttaataa
BBa_K1554003EaDAcTCodingValencia_UPV team members1092  . . . ttgggtacaagattcgtgtgcggcaattga
BBa_K1554004Yellow biosafety module for plantsDeviceValencia_UPV team members3394  . . . attcctaaaaccaaaatccagtgacctcgc
BBa_K1554005Blue biosafety module for plantsDeviceValencia_UPV team members3364  . . . attcctaaaaccaaaatccagtgacctcgc
BBa_K1742000Avena sativa LOV2 domainCodingRuben Escriba Piera456  . . . attgataaggcagtcgatacttgggtctga
BBa_K1742001Entertoxin LTBCodingRuben Escriba Piera414  . . . gatgagctacatcatcatcatcatcattga
BBa_K1742003Dronpa 145KCodingIvn Casas Rodrigo7413 . . . gctaagcccaagaagaagaggaaggtgtga
BBa_K1742004PhiC31 Plant codon optimizedCodingIvn Casas Rodrigo1836  . . . gtggcagcaccaaaaaaaaagaggaaggtg
BBa_K1742005BxBI integraseCodingIvn Casas Rodrigo1693  . . . gttgagcgattgcacacgggtatgtcttga
BBa_K174200935S:BxbI:T35S - 35S:ReporterBxBI::GFP:T35SDevicePilar Baldominos2964  . . . acacgggggactaaaaaaatggcttcctcc
BBa_K174201135S:LacIBDBD-DronpaK:T35S - 35S:DronpaN-VP16:T35SDeviceMnica Victoria Gutirrez Salazar2592  . . . gatgcccttggaattgacgagtacggtggg
BBa_K174201035S:LexABD-DronpaK:T35S - 35S:DronpaN-VP16:T35SDevicePilar Baldominos3176  . . . caggctaaacctaaaaagaagagaaaagtt
BBa_K174201235S:Gal4BD-DronpaK:T35S - 35S:DronpaN-VP16:T35SDeviceMnica Victoria Gutirrez Salazar3610  . . . gatgcccttggaattgacgagtacggtggg
BBa_K1618029Acyltransferase Candidate 1 -Fused to GFP -Expression ConstructCompositeLeda Coelewij3134  . . . ccaaaatccagtactaaaatccagatcgct
BBa_K1618030Acyltransferase Candidate 2 -Fused to GFP -Expression ConstructCompositeLeda Coelewij3407  . . . ccaaaatccagtactaaaatccagatcgct
BBa_K1618031Acyltransferase Candidate 3 -Fused to GFP -Expression ConstructCompositeLeda Coelewij3485  . . . ccaaaatccagtactaaaatccagatcgct
BBa_K1618032Acyltransferase Candidate 4 -Fused to GFP -Expression ConstructCompositeLeda Coelewij3659  . . . ccaaaatccagtactaaaatccagatcgct
BBa_K1618033Acyltransferase Candidate 1 Expression ConstructCompositeLeda Coelewij2396  . . . ccaaaatccagtactaaaatccagatcgct
BBa_K1618034Acyltransferase Candidate 2 Expression ConstructCompositeLeda Coelewij2669  . . . ccaaaatccagtactaaaatccagatcgct
BBa_K1618035Acyltransferase Candidate 3 Expression ConstructCompositeLeda Coelewij2747  . . . ccaaaatccagtactaaaatccagatcgct
BBa_K1618036Acyltransferase Candidate 4 Expression ConstructCompositeLeda Coelewij2921  . . . ccaaaatccagtactaaaatccagatcgct
BBa_K174201335S:PhiC31:T35S - 35S:ReporterPhiC31::GFP:T35SDeviceRuben Escriba Piera5508  . . . ttcctaaaaccaaaatccagtgacctcgct
BBa_K2017000C-split Cas9 + DnaE C-inteinProtein_DomainMonica Victoria Gutierrez Salazar2358  . . . aaggtgcccaagaagaagaggaaggtgtga
BBa_K2017001N-split Cas9 + DnaE N-inteinProtein_DomainMonica Victoria Gutierrez Salazar2241  . . . gatttgatgagggtggacaacctccctaac
BBa_K201700735s:5'+ Ga20ox consense + SAGTI-Luciferase + TnosDeviceMonica Victoria Gutierrez Salazar3057  . . . gttactagatcggcaattccgctagagacc
BBa_K201700835s + Ga20ox consense + RSIAT-Luciferase + TnosDeviceMonica Victoria Gutierrez Salazar2836  . . . gttactagatcggcaattccgctagagacc
BBa_K201700935s + Ga20ox consense + AEK-Luciferase + TnosDeviceMonica Victoria Gutierrez Salazar2872  . . . gttactagatcggcaattccgctagagacc
BBa_K201701135s:5' + TFL consense + SAGTI-Luciferase + TnosDeviceMonica Victoria Gutierrez Salazar3057  . . . gttactagatcggcaattccgctagagacc
BBa_K2017010 35s + Ga20ox consense + RSIAT-TEV-Luciferase + TnosDeviceMonica Victoria Gutierrez Salazar2869  . . . gttactagatcggcaattccgctagagacc
BBa_K201701235s + TFL consense + RSIAT-Luciferase + TnosDeviceMonica Victoria Gutierrez Salazar2836  . . . gttactagatcggcaattccgctagagacc
BBa_K2017013 35s + TFL consense + AEK-Luciferase + TnosDeviceMonica Victoria Gutierrez Salazar2872  . . . gttactagatcggcaattccgctagagacc
BBa_K2017014 35s + TFL consense + RSIAT-TEV-Luciferase + TnosDeviceMonica Victoria Gutierrez Salazar2869  . . . gttactagatcggcaattccgctagagacc
BBa_K226900935s: PhyB: VP16:NLS: TnosCompositeJos lvaro Ballesteros Gonzlez3970  . . . gataaatgcttcaataatgggaccgactcg
BBa_K4799990pAtMRP3RegulatoryYouran Yao, Wanjun Chen1651-1 . . . tattctcctcctccaaaactcgcctccata
BBa_K4213000Plant Thiamine Pyrophosphate RiboswitchRegulatoryIoannis Retalis675-1 . . . tacagccataaaagaagtctttaactcgct
  • The parts above have been tested or are intended to be used in Nicotiana benthamiana.
  • To add to this list, please use the category //chassis/eukaryote/nbenthamiana


iGEM Teams that have worked with Nicotiana benthamiana
  • [http://2016.igem.org/Team:Valencia_UPV Valencia UPV 2016 Wiki] | Parts
  • [http://2015.igem.org/Team:NRP-UEA-Norwich NRP-UEA-Norwich 2015 Wiki] | Parts
  • [http://2015.igem.org/Team:Valencia_UPV Valencia UPV 2015 Wiki] | Parts
  • [http://2014.igem.org/Team:NRP-UEA-Norwich NRP-UEA-Norwich 2014 Wiki] | Parts
  • [http://2014.igem.org/Team:Valencia_UPV Valencia UPV 2014 Wiki] | Parts


Marchantia polymorpha

"Marchantia polymorpha is [a] novel, eukaryotic multicellular chassis. Being a liverwort, it is one of the most primitive land plants around. Its small size and relative genetic simplicity make it easy to work with and an exciting new model organism in synthetic biology. Content to grow on agar plates, marchantia can be bioengineered in a standard lab with minimal extra equipment." - [http://2014.igem.org/Team:Cambridge-JIC Cambridge-JIC 2014]



More...
NameDescriptionTypeCreated bylengthusesseq
BBa_K1484215nopaline synthase terminatorTerminatorSalil Bhate29310 . . . gttactagatcggcaattccgctagagacc
BBa_K1484000AsPink chromoprotein in GoldenGate standardCodingVirginie Rutten721  . . . gctgggtcataattaataagctttgagacc
BBa_K1484001tsPurple chromoprotein in GoldenGate standardCodingVirginie Rutten709  . . . ggaaaaagcgacgtaataagctttgagacc
BBa_K1484002AmajLime chromoprotein in BBa and MoClo standardCodingVirginia Rutten711  . . . cggtcgtgccgttctaataactttgagacc
BBa_K1484104N7 nuclear localisation tagTagSalil Bhate262  . . . tgatcaagaagaagagtaaggtatgagacc
BBa_K1484106LTI membrane localisation tagCodingSalil Bhate238  . . . ttatatcatcaccttttgaggtatgagacc
BBa_K1484214MpEF1a (Marchantia poylmorpha Elongation Factor 1, PlantSyntax Pro+5U)RegulatorySalil Bhate1756  . . . ctgcacaaaggttgtcaccaatgtgagacc
BBa_K1484316Mp Selection CassetteTemporarySalil Bhate3559  . . . ttactagatcggcaattcgcaaaagtcttc
  • The parts above have been tested or are intended to be used in Marchantia polymorpha.
  • To add to this list, please use the category //chassis/eukaryote/mpolymorpha


iGEM Teams that have worked with Marchantia polymorpha
  • [http://2015.igem.org/Team:Cambridge-JIC Cambridge-JIC Wiki 2015] | Parts
  • [http://2014.igem.org/Team:Cambridge-JIC Cambridge-JIC Wiki 2014] | Parts


Arabidopsis thaliana

"Arabidopsis thaliana ... is easy to transform via R. radiobacter and has a short regeneration time." - [http://2014.igem.org/Team:Hannover Hannover 2014]


More...
NameDescriptionTypeCreated bylengthusesseq
BBa_K1478000Expa4 plant secretion signal, localizes to extracellular spaceProtein_DomainFabian Frmling60  . . . acatttgttctttttagcctcgccgacgct
BBa_K1478001Cellulose binding domainProtein_DomainFabian Frmling3093 . . . tacgttgaatttggatttgcgtcaggccgt
BBa_K382000pORE Open Series Vector with BioBrick MCS (Basta resistance)PlasmidChristina Agapakis7208  . . . tagaccagttacccagatctgaggcgcgcc
BBa_K382001pORE Open Serices Vector with BioBrick MCS (Kan resistance)Plasmid_BackboneChristina Agapakis7451  . . . ccttcttgacgagttcttctgaggcgcgcc
BBa_K382003pORE Expression Series Vector with BioBrick MCS (Kan reistance)PlasmidChristina Agapakis7969  . . . ccttcttgacgagttcttctgaggcgcgcc
BBa_K382002pORE Expression Series Vector with BioBrick MCS, Basta resistancePlasmidMara C. Inniss7786  . . . tagaccagttacccagatctgaggcgcgcc
BBa_K382004pORE Gus Reporter Series Vector with BiOBrick MCS (Kan resistance)Plasmid_BackboneChristina Agapakis9295  . . . ccttcttgacgagttcttctgaggcgcgcc
BBa_K382005pORE GFP Reporter Series Vector with BioBrick MCS (Kan resistance)Plasmid_BackboneChristina Agapakis8203  . . . ccttcttgacgagttcttctgaggcgcgcc
BBa_K382010amiRNA Construct for lycopene epsilon cyclase (LUT2)RNAPatrick Boyle413  . . . tttatctttatttaaggcatcgccatgggg
BBa_K382012amiRNA Construct for lycopene beta cyclase (LYC)RNAChristina Agapakis413  . . . tttatctttatttaaggcatcgccatgggg
BBa_K382011amiRNA Construct for carotene beta-ring hydroxylase (BETA-OHASE 1)RNAMara C. Inniss413  . . . tttatctttatttaaggcatcgccatgggg
BBa_K382014ihpRNA construct against Arabidopsis Bet v 1 allergenRNAChristina Agapakis1288  . . . gacgtctacctcaatctcctcttctatcat
BBa_K382020Arabidopsis optimized BrazzeinCodingPatrick Boyle165  . . . ctccagtgtatctgtgattattgtgagtat
BBa_K382021Arabidopsis optimized MiraculinCodingPatrick Boyle660  . . . gctttcgagttcaacaaaaccgtttatttc
BBa_K382015ihpRNA construct against Arabidopsis LTP AllergenRNAChristina Agapakis1372  . . . caagcatgccaacttcatcactccagccat
BBa_K382022pENTCUP2 plant specific promoterRegulatoryPatrick Boyle503  . . . ctcatcatcctcacctcaaaacccaccgga
BBa_K382017ihpRNA construct against Arabidopsis LTP AllergenRNAChristina Agapakis424  . . . tttatctttatttaaggcatcgccatgggg
BBa_K382018PDK intronIntermediateChristina Agapakis793  . . . attgattacagttgggaaattgggttcgaa
BBa_K382019ihpRNA construct against Arabidopsis Ger3 allergenRNAChristina Agapakis1429  . . . atcatcttcatactagtagcggccgctgca
BBa_K382034Act2pLacO (Actin promoter + 2x LacO)RegulatoryMara C. Inniss1241  . . . ctctttttgtgtgtttgcagctcataaaaa
BBa_K382025Arabidopsis optimized Miraculin with StrepII N-terminus tagCodingPatrick Boyle696  . . . gctttcgagttcaacaaaaccgtttatttc
BBa_K382026Arabidopsis optimized Brazzein with StrepII N-terminusCodingPatrick Boyle201  . . . ctccagtgtatctgtgattattgtgagtat
BBa_K382040Arabidopsis optimized Miraculin+2xYFPCodingMara C. Inniss2160  . . . gatgaactatacaaaggttctggtaccgca
BBa_K382041Arabidopsis optimized Brazzein+2xYFPCodingMara C. Inniss1665  . . . gatgaactatacaaaggttctggtaccgca
BBa_K382027Arabidopsis optimized Miraculin with StrepII C-terminus tagCodingPatrick Boyle696  . . . agtgcttggtctcacccacaattcgaaaag
BBa_K382028Arabidopsis optimized Brazzein with StrepII C-terminusCodingPatrick Boyle201  . . . agtgcttggtctcacccacaattcgaaaag
BBa_K1478002Top metal binding protein. Binds zinc, arsenic, copper and cadmium.CodingFabian Frmling909  . . . tgcggatcatcttgcagctgtaagtaataa
BBa_K1982002Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)CodingZexu Li1854  . . . actacaagtttgggaaaaaatggttgcaaa
BBa_K1982003CIBN(the N-terminal fragment of CIB1)CodingZexu Li612  . . . ccatacgatgttccagattacgcttaataa
BBa_K1982010CRY2-VP64(Eukaryotic LACE system)CodingZexu Li2100  . . . gactacaaggacgacgacgacaaataataa
BBa_K1982009Eukaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)CodingZexu Li1848  . . . actacaagtttgggaaaaaatggttgcaaa
BBa_K4213000Plant Thiamine Pyrophosphate RiboswitchRegulatoryIoannis Retalis675-1 . . . tacagccataaaagaagtctttaactcgct
  • The parts above have been tested or are intended to be used in Arabidopsis thaliana.
  • To add to this list, please use the category //chassis/eukaryote/athaliana


iGEM Teams that have worked with Arabidopsis thaliana
  • [http://2015.igem.org/Team:CAU_China CAU_China 2015] | Parts
  • [http://2015.igem.org/Team:Waterloo Waterloo 2015] | Parts
  • [http://2014.igem.org/Team:Hannover Hannover 2014] | Parts
  • [http://2010.igem.org/Team:Harvard Harvard 2010] | Parts



Physcomitrella patens

"The moss Physcomitrella patens belongs to the land plant division Bryophyta, which are one of the earliest representatives of the land plants (Embryophyta) having evolved from green algae about 470 million years ago during the early Paleozoic...The general organization of plant tissue into roots, stem and leaves is found in a much more basic version in mosses. They show a differentiated stem with simple leaves, usually only a single layer of cells thick and lacking veins, that are used to absorb water and nutrients. Instead of roots they have similar threadlike rhizoids. These have a primary function as mechanical attachment rather than extraction of soil nutrients.

However different mosses and vascular plants are because of the early diverge of the evolutionary lineages, they share fundamental genetic and physiological processes. Here researchers chose Physcomitrella patens as a model organism with a genome size of about 450 Mb along 27 chromosomes that is highly similar to other land plants in both exon-intron-structure and codon usage." - [http://2013.igem.org/Team:TU-Munich TU-Munich 2013]


More...
NameDescriptionTypeCreated bylengthusesseq
BBa_K1159000Erythromycin Esterase Type II (EreB) in RFC[25]CodingLouise FUNKE12542 . . . aagtcatctgtatctgaggtcgtttatgaa
BBa_K1159001NanoLuc Luciferase in RFC[25]CodingTU Munich 20135104 . . . ggttggagactttgcgagagaatccttgct
BBa_K1159003Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]CodingTU Munich 20135221 . . . ttctctgaagccgcctgcaaggtcaacaat
BBa_K1159010Secretory NanoLuc Luciferase (SERK-SigP_nLuc) in RFC[25] N-PartCodingTU Munich 2013616  . . . ggttggagactttgcgagagaatccttgct
BBa_K1159303Signal Peptide of SERK Receptor from Physcomitrella patens in RFC[25]Protein_DomainTU Munich 20131009 . . . tctaacgctgagggtgatgctcttaacacc
BBa_K1159304Signal Peptide of Ig Kappa chain from Mus musculus in RFC[25] N-partProtein_DomainTU Munich 2013676 . . . ttgctttgggttccaggatctaccggcgat
BBa_K1159305Transmembrane domain of SERK Receptor from Physcomitrella patens in RFC[25]Protein_DomainTU Munich 20131862 . . . tggtggagaagaagaaggcctattgaggct
BBa_K115930735S Terminator of Cauliflower Mosaic Virus (CaMV)TerminatorTU Munich 20132179 . . . tctaattcctaaaaccaaaatccagtgacc
BBa_K1159308Neomycin phosphotransferase II (nptII) plant expression cassette for G418 resistanceDeviceDong-Jiunn Jeffery TRUONG15171 . . . tactagatcgggcctcctgtcaagcttgat
BBa_K1159006Secretory NanoLuc Luciferase (IgKappa-SigP_nLuc) in RFC[25] N-PartCodingTU Munich 20135833 . . . ggttggagactttgcgagagaatccttgct
BBa_K1159315TMD of SERK Receptor from Physcomitrella patens with C-terminal GFP fusion in RFC[25]CodingTU Munich 20139331 . . . attacacatggcatggatgagctctacaaa
BBa_K1159316SERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]CodingTU Munich 20139968 . . . attacacatggcatggatgagctctacaaa
BBa_K1159314TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-PartCodingDong-Jiunn Jeffery TRUONG2921 . . . tggtggagaagaagaaggcctattgaggct
BBa_K1159120Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)Translational_UnitDong-Jiunn Jeffery TRUONG6751  . . . attaccctgggtatggatgagctgtataaa
BBa_K1159202Secretory SpyCatcher (IgKappa-SigP_SpyCatcher) in RFC[25] N-PartCodingDong-Jiunn Jeffery TRUONG4121 . . . ggcaaagcaactaaaggtgacgctcatatt
BBa_K1159203Secretory SpyTag (IgKappa-SigP_SpyTag) in RFC[25] N-PartCodingDong-Jiunn Jeffery TRUONG1121 . . . gtcatggttgatgcttacaagccaactaag
BBa_K1159204Secretory SpyCatcher (SERK-SigP_SpyCatcher) in RFC[25] N-PartCodingDong-Jiunn Jeffery TRUONG4451 . . . ggcaaagcaactaaaggtgacgctcatatt
BBa_K1159205Secretory SpyTag (SERK-SigP_SpyTag) in RFC[25] N-PartCodingDong-Jiunn Jeffery TRUONG1451 . . . gtcatggttgatgcttacaagccaactaag
BBa_K1159206Membrane-anchored SpyCatcher in RFC[25] N-PartCodingDong-Jiunn Jeffery TRUONG14472 . . . attacacatggcatggatgagctctacaaa
BBa_K1159207Membrane-anchored SpyTag in RFC[25] N-PartCodingDong-Jiunn Jeffery TRUONG11472 . . . attacacatggcatggatgagctctacaaa
BBa_K1825000UNIK antifreeze proteinCodingAdam Petersen324  . . . aagatctccggttgtactttcagcgctaac
BBa_K1825005nptIICodingJonathan Asmund Arnesen7951 . . . ttctatcgccttcttgacgagttcttctga
BBa_K1159015Membrane-anchored NanoLuc Luciferase in RFC[25] N-PartCodingTU Munich 20131585  . . . attacacatggcatggatgagctctacaaa
BBa_K1159306Actin5 Promoter from Physcomitrella patensRegulatoryIngmar Polte1948  . . . ggtggttgttgtgcaggtccgtattaaata
BBa_K182500435s CaMVRegulatoryJonathan Asmund Arnesen3905 . . . atataaggaagttcatttcatttggagagg
BBa_K182500635s CaMV + nptIICompositeJonathan Asmund Arnesen1191  . . . ttctatcgccttcttgacgagttcttctga
BBa_K1825008Stilbene synthaseCodingJonathan Asmund Arnesen1170  . . . acagttgttttacgttcaatggctatttaa
  • The parts above have been tested or are intended to be used in Physcomitrella patens.
  • To add to this list, please use the category //chassis/eukaryote/ppatens


iGEM Teams that have worked with Physcomitrella patens
  • [http://2015.igem.org/Team:UNIK_Copenhagen UNIK_Copenhagen 2015] | Parts
  • [http://2013.igem.org/Team:TU-Munich TU-Munich 2013] | Parts
  • [http://2011.igem.org/Team:UEA-JIC_Norwich UEA-JIC_Norwich 2011] | Parts



Nicotiana tabacum


There are no parts for this table

  • The parts above have been tested or are intended to be used in Nicotiana tabacum.
  • To add to this list, please use the category //chassis/eukaryote/ntabacum


iGEM Teams that have worked with Nicotiana tabacum
  • [http://2015.igem.org/Team:Georgia_State Georgia_State 2015] | Parts
  • [http://2015.igem.org/Team:NYMU-Taipei NYMU-Taipei 2015] | Parts
  • [http://2014.igem.org/Team:Hannover Hannover 2014] | Parts
  • [http://2010.igem.org/Team:Nevada Nevada 2010] | Parts



Rhizobium radiobacter/Agrobacterium tumefaciens

Rhizobium radiobacter/Agrobacterium tumefaciens is used as a shuttle chassis to deliver constructs into different plant chassis. "[Rhizobium radiobacter] infects plants through its Ti plasmid. The Ti plasmid inserts a segment of its DNA, termed T-DNA, into the host organisms genome when the T-DNA is successfully integrated this causes the expression of the gene of interest by the plant. The T-DNA can be altered to include any gene construct that you would like, so it can be inserted into the host cells." - [http://2014.igem.org/Team:NRP-UEA-Norwich/Project_System NRP-UEA-Norwich 2014]


iGEM Teams that have worked with Rhizobium radiobacter/Agrobacterium tumefaciens
  • [http://2015.igem.org/Team:AHUT_China AHUT_China 2015] | Parts
  • [http://2015.igem.org/Team:CAU_China CAU_China 2015] | Parts
  • [http://2015.igem.org/Team:Georgia_State Georgia_State 2015] | Parts
  • [http://2015.igem.org/Team:NRP-UEA-Norwich NRP-UEA-Norwich 2015] | Parts
  • [http://2015.igem.org/Team:Valencia_UPV Valencia_UPV 2015] | Parts
  • [http://2015.igem.org/Team:Waterloo Waterloo 2015] | Parts
  • [http://2014.igem.org/Team:NRP-UEA-Norwich NRP-UEA-Norwich 2014] | Parts
  • [http://2014.igem.org/Team:Hannover Hannover 2014] | Parts
  • [http://2012.igem.org/Team:Toronto Toronto 2012]
  • [http://2012.igem.org/Team:Kyoto Kyoto 2012]
  • [http://2010.igem.org/Team:Harvard Harvard 2010]



Chlamydomonas reinhardtii

iGEM Teams that have worked with Chlamydomonas reinhardtii
  • [http://2011.igem.org/Team:UEA-JIC_Norwich UEA-JIC_Norwich 2011]
  • [http://2016.igem.org/Team:USP_UNIFESP-Brazil USP_UNIFESP-Brazil 2016]
  • [http://2016.igem.org/Team:Linkoping_Sweden Linkoping_Sweden 2016]
  • [http://2016.igem.org/Team:SZU-China SZU-China 2016]
  • [http://2016.igem.org/Team:FAFU-CHINA FAFU-CHINA 2016]
  • [http://2016.igem.org/Team:Cambridge-JIC Cambridge-JIC 2016]
  • Humboldt Berlin 2019


Other Resources

  • [http://openplant.org/ OpenPlant]
  • Golden Braid
  • [http://synbio.tsl.ac.uk/golden-gate/ SynBio @ TSL]