Coding

Part:BBa_K382041

Designed by: Mara C. Inniss   Group: iGEM10_Harvard   (2010-10-26)

Arabidopsis optimized Brazzein+2xYFP

Arabidopsis optimized Brazzein with a 2xYFP tag.

We characterized[http://2010.igem.org/Team:Harvard/flavor/results] expression of this part in E. coli.

YFP tag performance:
Mir BrazzYFP Fig 1-crop.jpeg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830
    Illegal BsaI.rc site found at 1580


[edit]
Categories
//cds
//cds/reporter/yfp
//chassis/eukaryote/athaliana
//chassis/prokaryote/ecoli
Parameters
None