Part:BBa_K1467203

GoldenGate compatible Bax - cell death inducer

BAX coding sequence (Bcl-2-associated X) protein is a pro-apoptotic member of the Bcl-2 protein family which induces cell death.The part has been made compatible with the GoldenGate MoClo Assembly Standard as it is free from internal BsaI and BpiI recognition sequences.

iGEM14_NRP-UEA-Norwich chose to use this part as one of their reporters with results being detectable to the naked eye. On activation of the promoter, the protein is transcribed, driving cell apoptosis that is visible to the naked eye.

Figure 1: The result of an infiltration of Agrobacterium tumefaciens into Nicotiana benthamiana, showing plant cell apoptosis caused by expression of Bax.

Image (A) provides a control, showing a Nicotiana benthamiana leaf infiltrated (red circles) with Agrobacterium tumefaciens whilst B shows a Nicotiana benthamiana leaf infiltrated (red circles) with Agrobacterium tumefaciens carrying the plasmid encoding: 35s Constututive Promotor (Cauliflower Mosaic Virus) + Bax Coding Sequence (Mus Musculus) + Octopine Synthase Terminator (Agrobacterium tumefaciens).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 161
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 65
  
• 1000
  COMPATIBLE WITH RFC[1000]