Protein coding sequences/Membrane

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Receptors

Receptors tend to have three domains:

  1. An extracellular domain for binding ligands such as proteins, peptides or small molecules
  2. A transmembrane domain
  3. An intracellular or cytoplasmic domain which frequently can participate in some sort of signal transduction event such as phosphorylation


More...
NameProteinDescriptionTagDirectionUniProtKEGGLengthStatus
BBa_J07009ToxR toxicity-gene activator from Vibrio choleraeNoneForwardP15795 630In stock
BBa_K133063 (TIR)TLR3  453It's complicated
BBa_K133064 (TIR)TLR9  585It's complicated
BBa_K133065 (TMTIR)TLR3  600It's complicated
BBa_K133069 (TMTIR)TLR3stop  603It's complicated
BBa_K133067 (TMTIR)TLR4  621It's complicated
BBa_K133060 (TMTIR)TLR9  645It's complicated
BBa_K209400 AarI B-C part, hM4D  1434It's complicated
BBa_K209401 AarI B-C part, Rs1.3  1407It's complicated
BBa_K2627002 Advanced CcaS with PAS domain knocked out  1389It's complicated
BBa_K2627001 Advanced CcaS with PAS domain knocked out  1410It's complicated
BBa_K2627000 Advanced CcaS with PAS domain knocked out  1407It's complicated
BBa_K748001 AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.  1296It's complicated
BBa_K3183005 AgrC2 Quorum Sensing Histidine Kinase; C. difficile  1314Not in stock
BBa_K5466024 antiAFB1-scFv1 CadC  1272 
BBa_K5466018 antiAFB1-scFv1 NubG  1410 
BBa_K5466025 antiAFB1-scFv2 CadC  1272 
BBa_K5466019 antiAFB1-scFv2 Cub  2322 
BBa_I712002 CCR5  1059It's complicated
BBa_I712003 CCR5-NUb  1194It's complicated
BBa_I712010 CD4 sequence without signal peptide  1299It's complicated
BBa_I712017 Chemokine (CXC motif) receptor 4, fused to N-terminal half of ubiquitin.  1191It's complicated
BBa_K4025001This CDS expresses the intermembrane one-component protein ChiSChiS inter-membrane protein  3390 
BBa_K1908001 Coding sequence for Homo sapien Cripto-1 membrane protein.  767It's complicated
BBa_I15010Cph8cph8 (Cph1/EnvZ fusion)NoneForward  2263In stock
BBa_K592000 cph8 (Cph1/EnvZ fusion)  2238In stock
BBa_I728500 CPX Terminal Surface Display Protein with Polystyrene-Binding Peptide  654It's complicated
BBa_J52035 dnMyD88  420In stock
BBa_K1909003 EnvZ Signal Peptide and first Transmembrane Domain  161It's complicated
BBa_K1909005 EnvZ Signal Transduction and Kinase Domains  882It's complicated
BBa_K259000 fhuA - Outer membrane transporter for ferrichrome-iron  2247It's complicated
BBa_K976000 Fibroblast Growth Factor-1 Receptor Coding Sequence  2466Not in stock
BBa_K259001 fiu – Outer Membrane Ferric Iron Transporter  2247It's complicated
BBa_J58104 Fusion protein Trg-EnvZ for signal transduction  1485It's complicated
BBa_K1897002 HasR Membrane receptor coding sequenceForward  2700Not in stock
BBa_K137112 lamB  1339In stock
BBa_K1909002 mTaz (Taz-derived mDAP receptor)  1452It's complicated
BBa_K4345007 NarX  1794 
BBa_K4345008 NarX H399-E  1797 
BBa_K1909004 NOD1 LRR Domain  969It's complicated
BBa_C0082tar-envZReceptor, tar-envZLVAForward  1516In stock
BBa_J58105 Synthetic periplasmic binding protein that docks a vanillin molecule  891In stock
BBa_K845005 TapZ engineered membrane receptor  1449Not in stock
BBa_K533007 Tar receptor  1662It's complicated
BBa_K909008 tetR-DBD-UVR8 fusion protein  1674In stock
BBa_K1315002 The rpfC gene from Xanthomonas campestrisForward  2181It's complicated
BBa_I712012 TIR domain of TLR3  456In stock
BBa_K389001 VirA receptor  2505It's complicated
BBa_K143037 YtvA Blue Light Receptor for B.subtilis  789It's complicated


Transporters

Transporters are membrane proteins responsible for transport of substances across the cell membrane.


More...
NameProteinDescriptionTagDirectionUniProtKEGGLengthStatus
BBa_J07006 malE  1191It's complicated
BBa_J07017 FecA protein  2325It's complicated
BBa_J92000 Lead Uptake Transporter   1947Not in stock
BBa_K1145001 A transporter which is involved in transporting atrazine.  1341It's complicated
BBa_K1172401 mtrCAB - gene cluster from S. oneidensis for cytochrome expression  5112It's complicated
BBa_K141000UCP1Ucp1  924In stock
BBa_K141002 Ucp 175 deleted  921In stock
BBa_K141003 Ucp 76 deleted  921In stock
BBa_K1709005Ag43Ag43-YFP  3843Not in stock
BBa_K190028 GlpF  846In stock
BBa_K2247002 HXT2 glucose transpoter from S. cerevisiae  1623It's complicated
BBa_K2247003 GFP tagged HXT2 glucose transpoter from S. cerevisiae  2349It's complicated
BBa_K2247004 HXT5 glucose transpoter from S. cerevisiae  1776It's complicated
BBa_K2247005 GFP tagged HXT5 glucose transpoter from S. cerevisiae  2502It's complicated
BBa_K2326001 Glutamate/GABA antiporter  1506It's complicated
BBa_K2455003 Cell-Penetrating USER CassetteC-terminal hexa-histidineForward  81It's complicated
BBa_K2455004 YddG E. coli Tyr, Trp, Phe exporter Forward  885It's complicated
BBa_K3016031TatCVibrio natriegens' native tatC CDS  747Not in stock
BBa_K3848008 LLDP  1652 
BBa_K4025002 ChiP (chitoporin)  1050 
BBa_K4214000 Human Gastric Intrinsic Factor (hGIF)  1211 
BBa_K4252005 Phosphate ABC transporter membrane subunit PstS.  1041 
BBa_K4252006 Phosphate ABC transporter membrane subunit PstC.  960 
BBa_K4252009 Phosphate-specific transportor (Pst)   3952 
BBa_K4781002 Tat A - Tat pathway protein  270 
BBa_K4781003 Tat B - A protein of the TAT pathway complex  516 
BBa_K4781004 Tat C - A protein of the TAT pathway complex  777 
BBa_K4781005 Tat E - A protein of the TAT pathway complex  204 
BBa_K629005 trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity  1869In stock
BBa_K633001 Linker + EstA Membrane Protein  876In stock


JaumeForesMartosPhoto.jpg
Jaume Forés Martos and [http://2008.igem.org/Team:Valencia 2008 Valencia iGEM team] designed and constructed several versions of the UCP1 transporter.

Channels

Channels are made up of proteins that form transmembrane pores through which selected ions can diffuse.


More...
NameProteinDescriptionTagDirectionUniProtKEGGLengthStatus
BBa_I746200 FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.  2208In stock
BBa_I765002 ExbB membrane spanning protein in TonB-ExbB-ExbD complex [ Escherichia coli K12 ]  735It's complicated
BBa_I765003 TonB ferric siderophore transport system, periplasmic binding protein TonB [ Pseudomonas entomophila  735It's complicated
BBa_K090000 Glutamate gated K+ channel  1194It's complicated
BBa_K2263000 mHCN2 - Hyperpolarization-activated cyclic nucleotide-gated 2  2592Not in stock
BBa_K2263001 hERG - human Ether-a-go-go-Related Gene K+ channel  3480It's complicated
BBa_K2263002 Kir2.1 Voltage-gated potassium channel  1293It's complicated
BBa_K2263003 α1G calcium ion channel  6888Not in stock
BBa_K284000 Lactate Permease from Kluyveromyces lactis  1873Not in stock
BBa_K559000 Halorhodopsin  640It's complicated
BBa_K559001 Halorhodopsin under T7 promoter  728It's complicated
BBa_K864204 OmpF  1092In stock


Pumps

Pumps are membrane proteins that can move substances against their gradients in an energy-dependent process known as active transport.


More...
NameProteinDescriptionTagDirectionUniProtKEGGLengthStatus
BBa_J22101 Lac Y gene  1288In stock
BBa_K079015 LacY transporter protein from E. coli  1254In stock
BBa_K1020006 YbhGFSR  4971It's complicated
BBa_K119003 RcnA (YohM)  833It's complicated
BBa_K137001 LacY  1254In stock
BBa_K1642001 Codon-optimized Halorhodopsin with His-tag  891Not in stock
BBa_K2596005 cscB (sucrose permease) from E. coli  1248Not in stock


Other membrane proteins

Other proteins and protein domains that are associated with the membrane but do not fall into one of the categories listed above are included here.


More...
NameProteinDescriptionTagDirectionUniProtKEGGLengthStatus
BBa_I712024 CD4  1374It's complicated
BBa_K1172501 Outer membrane porin OprF from Pseudomonas fluorescens  1298It's complicated
BBa_K133061 CD4ecto  1113It's complicated
BBa_K136046 envZ*  1353It's complicated
BBa_K1666000LsrALsrA of LuxS/AI-2 signaling pathway in Salmonalla  1536In stock
BBa_K1761001 Outer Membrane Protein X (OmpX) with BamHI-linker and mNeonGreen  1439In stock
BBa_K1761002 Outer Membrane Protein X (OmpX) with BsoBI-linker and NanoLuc  1241In stock
BBa_K1933000 N-terminal domain of BclA protein fused to 6xHis tag  84Not in stock
BBa_K1933001 INPNC fused to 6xHis tag  978It's complicated
BBa_K212700147 kDa subunitInducible High Expression His-Tagged Photosystem II CP47 subunitNickel Affinity Histine  2071It's complicated
BBa_K212700247 kDa subunitCP47 His-tagged with Lac PromoterNickel Affinity Histine  1886It's complicated
BBa_K2127003 psbT  555It's complicated
BBa_K212700447 kDa subunitCP-47-HIS TAG  1548Not in stock
BBa_K2168000 pBAD/araC - N(6xHis) Ag43   4511It's complicated
BBa_K2168001 pBAD/araC - (N-6xHis) - Ag43 - sfGFP  5258It's complicated
BBa_K227006 puc BA coding region of R. sphaeroidesforward  336It's complicated
BBa_K2549006 mouse Notch1 core  978It's complicated
BBa_K2549007 mouse Notch1 core (extended)  1089Not in stock
BBa_K2549008 human Notch1 core  1008It's complicated
BBa_K3013003 mamC-FbFP BS2 fusion  819Not in stock
BBa_K3588020 Synthetic Adhesin  2397Not in stock
BBa_K367000 prhA coding sequence  2316It's complicated
BBa_K4035001 CUP1 fused to Aga2 and tagged with a V5 epitopeForward  612Not in stock
BBa_K4035002 Dimerization of the copper metallothionein 1 : CUP1-(GGGGS)3-CUP1Forward  405Not in stock
BBa_K4035003 Dimerization of the copper metallothionein 1 : CUP1-(GGGGS)4-CUP1Forward  420 
BBa_K4035004 Dimerization of the copper metallothionein 1 : CUP1-(AP)7-CUP1Forward  402 
BBa_K4035005 Dimerization of the copper metallothionein 1 : CUP1-(EAAAK)3-CUP1Forward  405 
BBa_K4035006  Dimerization of the copper metallothionein 1 : CUP1-(EAAAK)4-CUP1 Forward  420 
BBa_K4035007 Dimerization of the copper metallothionein 1 : CUP1-GGGGSEAAAKGGGGS-CUP1 Forward  405 
BBa_K4035008 Dimerization of the copper metallothionein 1 : CUP1-GGGGS(EAAAK)2GGGGS-CUP1Forward  420 
BBa_K4765001 intimin  1968 
BBa_K4765008 INPNC  924 
BBa_K4765009 LCA  828 
BBa_K5213000rboh [Chondrus crispus]rboh [Chondrus crispus]  2481 
BBa_K5436124 Optimized RBS for BIND-System+BIND-bearPETase+6xHisTag  1377 
BBa_K5466017 Nb28-S102D Aga2P  681 
BBa_K777113MotAmotA  831In stock
BBa_K777117MotBmotB  927In stock
BBa_K808027 EstA : inactive fusion protein for bacterial surface expression  1440In stock
BBa_M12067 E1  264Not in stock