Part:BBa_K1642001

Codon-optimized Halorhodopsin with His-tag

Halorhodopsin (HR) proteins are light-driven inward-directed chloride pumps from halobacteria. They are membrane-integral proteins of the rhodopsin superfamily that form a covalent bond with the carotenoid-derived chromophore all-trans retinal. Absorption of a photon with a defined optimal wavelength induces trans-cis isomerization of retinal, which triggers a catalytic photocycle of conformational changes in the protein, resulting in the net import of one chloride per photon into the cytoplasm(Amezaga, J. M. et al. 2014) . We use it as our biodesalination driver which confers cyanobacteria the ability to absorb chloride to a significant degree. The sequence is codon-optimized so that it would be easier for cyanobacteria to translate the protein.

His-tag is used to produce anti-His mAb as a first antibody or to purify the protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 133
  Illegal NgoMIV site found at 292
  Illegal NgoMIV site found at 613
  Illegal AgeI site found at 484
  
• 1000
  COMPATIBLE WITH RFC[1000]