Coding

Part:BBa_K2455004

Designed by: Jon Fugl   Group: iGEM17_UCopenhagen   (2017-10-27)


YddG E. coli Tyr, Trp, Phe exporter

YddG is an aromatic amino acid exporter that is responsible for the secretion of Tryptophan, Tyrosine and Phenylalanine.

Previous work by Wang et al. have shown that over-expression of YddG in E.coli increases the accumulation of Tryptophan in the culture medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 577
    Illegal AgeI site found at 639
    Illegal AgeI site found at 756
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane/transporter
//chassis/prokaryote/ecoli
Parameters
chassisEscherichia coli
directionForward
functionTransporter
originEscherichia coli MG1655 - Codon optimized
targetTryptophan, Tyrosine, Phenylalanine