Coding

Part:BBa_K4765001

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-09-29)

intimin

contributed by Fudan iGEM 2023

Introduction

Intimin is a surface display system, it was first described by Piñero-Lambea et. Al[1]. and tested by iGEM22_GreatBay_SCIE in BBa_K4275026. It includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a 12-stranded beta-barrel for transmembrane insertion.

Usage and Biology

The protein attached to the C-terminus of intimin can achieve outer membrane localization. In our project, intimin is fused with antigens, nanobodies, and lectins, facilitating their display on the membrane of E.coli. This approach allows for the binding of E.coli-E.coli and E.coli to cyanobacteria.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1244


Reference

  1. ↑ Piñero-Lambea, C., Bodelón, G., Fernández-Periáñez, R., Cuesta, A. M., Álvarez-Vallina, L., & Fernández, L. Á. (2015). Programming controlled adhesion of E.coli to target surfaces, cells, and tumors with synthetic adhesins. ACS Synthetic Biology, 4(4), 463–473. https://doi.org/10.1021/sb500252a
[edit]
Categories
//cds/membrane
Parameters
protein