Coding

Part:BBa_J92000

Designed by: Justin D Smith   Group: UC Davis - BIM189C - S10   (2010-05-23)

Lead Uptake Transporter

This part was derived from the Pbr T lead uptake protein from Ralstonia metallidurans. The protein is responsible for the uptake of Pb 2+ ions. We also added a 6 His tag for characterization to the C terminal domain of the protein.

This part can be used for projects where one needs to uptake lead ions from the environment into the cell interior.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 591
    Illegal NgoMIV site found at 679
    Illegal NgoMIV site found at 975
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 51


[edit]
Categories
//cds/membrane/transporter
Parameters
None