Part:BBa_K2596005

cscB (sucrose permease) from E. coli

This is a sucrose-proton symporter from E. coli.

Usage and Biology

CscB is a membrane-bound sucrose-proton (hydrogen ion) symporter. This CscB is specifically codon-optimized for S. elongatus PCC 7942. It is compatible in many strains of Synechococcus elongatus, including 2973, 6803 and 7942. S. elongatus, like most cyanobacteria, naturally produce a basic environment. Because the symporter is hydrogen-ion dependent, sucrose flows along the gradient from inside the cell (high amount of hydrogen ions) to outside of the cell (low amount of hydrogen ions).

Generally, it is useful to express CscB with an inducible protein as secreting sugar leads to rerouting carbon from growth [1]. As such, when using this biobrick, it's important to separate the growth and secretion phases. Because the cyanobacteria are secreting sugar, they are also more prone to contamination.

References: [1] Daniel C. Ducat, J. Abraham Avelar-Rivas, Jeffrey C. Way, Pamela A. Silver. "Rerouting Carbon Flux To Enhance Photosynthetic Productivity." Appl. Environ. Microbiol. Mar 2012, 78 (8) 2660-2668; DOI: 10.1128/AEM.07901-11

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 481
  Illegal NgoMIV site found at 604
  
• 1000
  COMPATIBLE WITH RFC[1000]

Sucrose Assays

For experimental methods and results on characterization, look at the page for composite part BBa_K2596015.