Coding

Part:BBa_K2168000

Designed by: Arunavo Chakraborty   Group: iGEM16_IISc_Bangalore   (2016-10-13)


pBAD/araC - N(6xHis) Ag43

This part encodes E coli protein Ag43, with an additional 6xHis tag for easy purification and quantification. Ag43 expression is known to cause E coli cells to adhere together, and the 6xHis tag was added to allow for convenient study of variation of E coli aggregation with respect to Ag43 expression levels.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1963
    Illegal NgoMIV site found at 3187
    Illegal NgoMIV site found at 3697
    Illegal NgoMIV site found at 3718
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2713
    Illegal AgeI site found at 3457
    Illegal AgeI site found at 3871
    Illegal AgeI site found at 4113
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//cds/membrane/extracellular
//chassis/prokaryote/ecoli
//collections/biofilm
//plasmidbackbone/copynumber/high
//rnap/prokaryote/ecoli/sigma70
Parameters
biologyE coli
chassis
family
function
kegg
negative_regulators
positive_regulators
proteins
rbs
tag
uniprot