Part:BBa_K1145001
A transporter which is involved in transporting atrazine.
TRM is a transmembrane transporter which is involved in transporting atrazine from the outside to the inside of the bacteria. It comes from the genome of Arthrobacter aurescens. The length of its gene is 1341bp. By using this part, we hope to increase the amount of atrazine inside the bacteria so that our Atrazine-RBS and TrzN can function better.
This picture shows the atrazine concentration in supernatant of bacterial cultures, which were cultured for 24 hours in 37℃. Group 0 means no bacteria in the cultures; cultures in Group Wild Type contained the wild type K12 bacteria; cultures in Group Vector contained the strain K12 with pGFP; and cultures in Group TRM+ contained the bacteria which are able to express TRM. “*” means the difference between two groups which are connected by half square brackets is distinctive. From this chart we can conclude that TRM plays an important role in the transportation of atrazine.
Combining TRM and TrzN together can make the degradation more efficient. shows the atrazine concentration in supernatant of bacterial cultures, which were cultured for 24 hours in 37℃. Apart from groups shown above, cultures in Group TRM+TrzN+ contained the bacteria which are able to express TRM as well as TrzN. "*" means the difference between two groups which are connected by half square brackets is distinctive. From this chart we can see that the atrazine concentration in supernatant of Group TRM+TrzN+ was even distinctively less than that in supernatant of TRM+. This means combining TRM and TrzN is indeed a wise choice to polish up our system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 145
- 1000COMPATIBLE WITH RFC[1000]
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