Part:BBa_K4025002

ChiP (chitoporin)

ChiP is a chitoporin from Vibrio cholerae that is codon-optimised for E. coli. This protein is found in the outer membrane and allows chitin oligosaccharides that were degraded by chitinases in the extracellular space to enter the periplasm.

ChiP was transformed into plasmid backbone pSB1k01 and a growth curve was constructed to measure cell burden. See: https://2021.igem.org/Team:Lubbock_TTU/Contribution.

As can be seen from the growth curves, the L1 transcriptional unit with ChiP did not overly burdened cell growth– all transformed cells survived. It should be noted; however, that the OD600 readings of all cultures were not standardized prior to inoculation, seen by the varying y-intercepts of all growth curves. Because of this, this data should not be used as quantitative measurements of growth kinetics, but rather a qualitative demonstration of relative growth.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]