Difference between revisions of "Promoters/Catalog"

Revision as of 04:18, 30 October 2008

The registry has a large collection of Promoters available. We categorize promoters in two ways. First, most promoters are designed for a particular RNA polymerase (RNAP) holoenzyme, e.g E. coli RNAP bound to σ⁷⁰ (σ⁷⁰ is a sigma factor, one of a group of proteins that confers specificity on E. coli RNAP for particular promoter sequences), or the RNAP from the T7 bacteriophage. Second, promoters are categorized by the manner in which they are regulated. For example a promoter may be unregulated (constitutive), positively regulated (activatable) or negatively regulated (repressible). Regulated promoters allow you to "program" biological systems. For example, you can perform logic (e.g. "If protein X is present, promoter Y is off") or ensure that a particular gene is only expressed when a particular small molecule is present in the media.

The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.

Regulation	RNA Polymerase
	Prokaryotic								Bacteriophage		Eukaryotic
	E. coli				B. subtilis		Synechocystis sp	Salmonella	T7	SP6	General	Yeast
	σ70	σS	σ32	σ54	σA	σB
Positive Constitutive Negative Multiple	53	-	-	-	1	-	-	2	-	-	1	10
	59	2	2	-	2	3	-	2	12	1	2	10
	89	-	-	-	1	-	-	-	6	-	7	2
	110	-	-	1	-	-	1	-	-	-	4	1

We also have a collection of miscellaneous promoters available that are provided "as is". If you can contribute more information about these promoters, for example, by providing the information that will allow us to add them into one of the categories in the table above, please do so.