Collections/Best Composite Part

A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. Generally, the Best Composite Part Award is given to a "device," a composite part that can function independently, without the need for further assembly. The iGEM community can improve upon these devices, use them as part of their designs, and/or use them as a foundation for new devices.

The following collection contains parts (and teams) that won, or were nominated, for the Best Composite Part award. Generally, these parts:

  • are novel and/or provide a useful function
  • are highly documented and characterized, with data and visualizations that show they work as expected
  • adhere to the iGEM competition's requirements and the Registry's requirements for submissions


Note: These tables do not yet contain all award and nominees. We have updated these tables with information from iGEM Judging Forms (started in 2015), and awards from years before 2015 will need to be added manually.


Winners


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NameDescriptionTypeCreated bylengthusesseq
BBa_K1658000Cinnamyl alcohol dehydrogenase CAD1CompositeŞeniz Yüksel, Burak Kızıl1231  . . . tttatctgttgtttgtcggtgaacgctctc
BBa_K1689010 N-luc-dCas9CompositeZHANG Yihao56301 . . . ctgatgtcaagcgtgtacgaatttgataat
BBa_K1758377Biosensor device for detection of GHB and GBLCompositeTeam Bielefeld-CeBiTec 20151863  . . . aagcttgggtaccgatcgcagaaagactaa
BBa_K1890002Silicatein gene, fused to transmembrane domain of OmpA, with strong RBSCompositeLycka Kamoen, Maria Vazquez1481  . . . gctagtgatgcctcctaccccactctctag
BBa_K1991009Pcons-RBS-LO-AOX2-HisCompositeChen, Pei-En2662  . . . aggggttttttgctgaaaggaggaactata
BBa_K1993009CXCR4-T2A-Luciferase-IRES-eGFPCompositeSu Xiaojun3366  . . . actctcggcatggacgagctgtacaagtaa
BBa_K2206006 Toehold switch for hsa-miR-15b-5p with GFPmut3b and inducible promoterCompositeSammy Lovat2014  . . . catggcatggatgaactatacaaataataa
BBa_K2259091SynORI inducible plasmid copy number deviceCompositeLaurynas Karpus925  . . . accgttggtagcggtggtttttttgtttgc
BBa_K2306008Secretory-abundant heat soluble protein 33020 "SAHS 33020" (T7 promoter, RBS and double terminator)CompositeGuillermo Serena Ruiz736  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2753018TALE2 sp1CompositeWEI KUANGYI 3166  . . . tcagaccggaaagcacatccggtgacagct
BBa_K2770002ycdW generatorGeneratorLuca Brenker, Peter Gockel, Maria Musillo, Elena Nickels, Jan Benedict Spannenkrebs1060  . . . catcaccatcaccatcatcatcatcattaa
BBa_K2796028Exosome boosterCodingShuangshuang Pu38742 . . . tcccccggcaatcattacataaacagataa
BBa_K2932003PliaI + RBS + CA + terminatorCompositePing-Yi Chen1173  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2980009CIB1-GCN(4)-mEGFP-FUSLCDCodingJi Gao1713  . . . atcactctcggcatggacgagctgtacaag
BBa_K3187000P22 Bacteriophage Coat Protein with LPETGG Tag for Sortase-mediated LigationCompositeiGEM TU_Darmstadt 20191650  . . . tccggattggcgaatgggacgcgccctgta
BBa_K3352006T7 + RBS SplintR Ligase Expressing ConstructCompositeHannah Hsu1124-1 . . . caccttcgggtgggcctttctgcgtttata
BBa_K3370601T7 promoter + LacO + RBS + Harmonized GR with linker and GFP + 6x His-tag + TerminatorCompositeCHIH-LU CHIANG1877-1 . . . gactgtccacgacgctatacccaaaagaaa
BBa_K3407022Short hairpin RNA (shRNA) potential trigger of RNAi. Transcription controlled under T7 promoter.CompositeJavier Navarro Delgado87-1 . . . tgtaggtggcatcgccctcgccctcgccgg
BBa_K3512042Atmosphere Regulated KillswitchDeviceGourav Saha958-1 . . . caccttcgggtgggcctttctgcgtttata
BBa_K3758301T7 Universal Test Construct 7.0T7Michael Burgis823-1 . . . cccggtaggggcccacgcttgttggagacg
BBa_K3893030Population control device (QS-based lysis protein oscilator)CompositeStefania Soledad Montesinos Ludena3200-1 . . . ttcgggtgggcctttctgcgtttatacgct
BBa_K4011008CBM3-NT2RepCT-CBM3CodingZixiang Zhou1995-1 . . . aatggtgttctggtttggggtaaagaaccg
BBa_K4150006T7P-g10.RBS-His-Ova-T7TCompositeWEI CHIEH CHIN1363-1 . . . gcctctaaacgggtcttgaggggttttttg
BBa_K4156101pLldR RegulatoryZheng Huang1361-1 . . . gcacatccggtgacagctaaagaggagaaa
BBa_K4239008fiatlux genes with their promoter to emit luminescenceTranslational_UnitGuillaume FULCONIS5867-1 . . . ttaagcttaaccgaagcgtttgatagttga
BBa_K4735014NarK promoter fused to sfGFPCompositeAsha Keshavarz980-1 . . . tttatctgttgtttgtcggtgaacgctctc
BBa_K4735015dmsA promoter driving superfolder GFP expressionCompositeAsha Keshavarz979-1 . . . tttatctgttgtttgtcggtgaacgctctc


Nominees


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NameDescriptionTypeCreated bylengthusesseq
BBa_K1583102pRha + CsgA + Hydroxyapatite-affinity tagCodingStefan Robert Marsden, Hector Sanguesa Ferrer6841 . . . gtctccgcgtccgtaactactagaaggagg
BBa_K1598008J23101 promoter + RBS + Human FDXR + RBS + Human FDX1 + RBS + Human CYP11A1 + rrnb double terminatorCompositeYash Mishra3910  . . . gggaccaccgcgctactgccgccaggcaaa
BBa_K1610105pTS + RBS + yebF + ACT3m + TermCompositeLeon Yim2743  . . . caccttcgggtgggcctttctgcgtttata
BBa_K1639000Toehold-GFPCompositeMustafa Yılmaz987  . . . ggggttttttgctgaaaggaggaactatat
BBa_K1650006Constitutive promoter expressing GFP and constitutive promoter expressing RFPCompositeAnna Knoerlein1830  . . . caccttcgggtgggcctttctgcgtttata
BBa_K1692021PanK + hybrid promoter phaCABCompositeErica Lieberman51761 . . . caacggcggcctgcatatgggctgacctgc
BBa_K1720005 Human phosphodiesterase 5A gene silencing device NO.3DeviceYing Guan , Yuanbin Cui 304  . . . cgagtaattggcatacaaagaatgcttttt
BBa_K1806005T7+ ibPB RNA Thermometer + RFPCompositeİBRAHİM YASİR ORHAN976  . . . ggggttttttgctgaaaggaggaactatat
BBa_K182500635s CaMV + nptIICompositeJonathan Asmund Arnesen1191  . . . ttctatcgccttcttgacgagttcttctga
BBa_K1893016Arabinose inducible gp2 (pBAD+gp2)CompositeHenry Lloyd-Laney, Stefan Grossfurthner1568  . . . cgtgtgcgtccttgtgtagcaccgaagtaa
BBa_K1921021PETase+linker.b+GCW51CompositeZhuozhi Chen1404  . . . ggtggtgttgccattgccctattgatctag
BBa_K1954001Lycopene cassette under the control of NarK, an oxidative stress inducible promoterCompositeAbbie Rogan3532  . . . ggtttgatgctggaggatctgatataataa
BBa_K1963008Device for rhamnose-dependent expression of an OsmY fusion to E. coli Hfq..CompositeFrank Sargent1060  . . . tccgcgcaacaggacagcgaagaaaccgaa
BBa_K1974033T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-TagCompositeYU-CHUN WU101  . . . gaggtgcaccaccaccaccatcacgtgtaa
BBa_K1997011P+R->sHRP-N->FRB->RBS->FKBP->sHRP-C->TerCompositeXinyuan Qiu2019  . . . caccttcgggtgggcctttctgcgtttata
BBa_K1998000Mg-Chelatase PlasmidDeviceShauna Winchester10938  . . . gacaagattgagggagtcgaataataataa
BBa_K2082231RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434CompositePascal Schmidt1596  . . . ccccgcccctgacagggcggggtttttttt
BBa_K2170002Secretory eukaryotic biotin binding receptor with single chain avidin DeviceMax Mustermann3813  . . . cgtgaaccactgctcccttccctgtccttt
BBa_K2201373T3 polymerase with inverted mRFP under T3 promoter control for signal enhancingCompositeSvenja Vinke3411  . . . aagccattctccctttagtgagtgttaatt
BBa_K2230017Pcar-wRBS-PhlF-T-Pr-sRBS-GFP-sRBS-lysis-sRBS-NucA/pSB1C3DeviceYi-Lun Huang & Pei-Hong Chen2222  . . . tggagcgaagacaacgctgattcaggtcaa
BBa_K2282011AmilCP with DSbox under Upelmt/CspA promoter + 5'UTRCompositeEliott LAFON1033  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2315034LasR-pLas-GFP HSL inducible fluorescent actuator MeasurementFANG LUO, FANG BA1953  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2323004Lwa Cas13a under T7 promoter with solubility tagCompositeAurore Dupin4117  . . . ttgaggggttttttgctgaaaggaggaact
BBa_K2333434pLac0-1 mf-LonOtherEthan M Jones3944  . . . tcctcaatcgcactggaaacatcaaggtcg
BBa_K2387032CpxR-eYFPn[1-154] and CpxR-eYPFc[155-238] + araC/pBAD promoterCompositeBart Scholten3424  . . . actctcggcatggacgagctgtacaagtaa
BBa_K2398019Theophylline riboswitch - geneIII for application in Phage-assisted continous evolution (PACE) CodingMoritz Przybilla1314  . . . tgcctatcaactcgagtagtactaagatct
BBa_K2446028SV40_8_ZF_43-8 promoterCompositeYijie Pan446  . . . actactagagagtgaggacagagtgaggac
BBa_K2491027Combination of Part 1 and Part 2 of Phenanthrene Degradation - 100ProjectPhilippe Hansen-Estruch7241  . . . accttcgggtgggcctttctgcgtttatat
BBa_K2560272tfoX of V. cholerae CompositeMemduha Muratoglu1664  . . . gggcctttctgcgtttatacgcttgagacc
BBa_K2571006Dual Expression of FucO and GSHCompositeTugba İnanc, Ceyhun Kayihan 3644  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2581011Improved fatty acid acyl-CoA biosensor with weak RBSCompositeLaura Sans Comerma912  . . . aataacgctgatagtgctagtgtagatcgc
BBa_K2601011FKBP-yEGFP-HOTag3CompositeYang Jianzhao1164  . . . ctgaaagaaattgccaagtccctcaaaggg
BBa_K2621055CAT-Seq Esterase (Ready for Expression)CompositeLaurynas Karpus1692  . . . gccagttttacatgcttaaaagcataataa
BBa_K2675042OmpA-SAIRGA expression cassetteCompositeEsteban Lebrun224  . . . cgaaaggggggccttttttcgttttggtcc
BBa_K2705006PgltAB-LacI-Pgrac-TetATranslational_UnitDanqing Tong2790  . . . gtatataaacattctcaaagggatttctaa
BBa_K2715008Antisense RNA targeting C. difficile toxins composite 2CompositeDaniel Partridge519  . . . taaagaagagttaataaaactcgcatatag
BBa_K2738006Ovispirin fused to N-ter of Ferritin proteinCompositeDarshak Bhatt613  . . . gaattgtcgactcttgatacccagaattga
BBa_K2812005Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoterCompositeGuido Oerlemans, Maxime van den Oetelaar and Mariska Brüls 1496  . . . gcatcagcacaccaccaccaccaccactga
BBa_K2868015HHTC-Re-CBD x2 Copper BindingCompositeAdvait Patil660  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2885002Gold binding polypeptide (GBP) + Protein G (ProG) Fusion CompositeSungho Ko906  . . . caccttcgggtgggcctttctgcgtttata
BBa_K2933102His+Linker a+Sumo+Linker b+SPG-1CompositeDongxu Li12131 . . . caacaagcggcgaatgcggatcgtcgctaa
BBa_K3007029Expression device of dsRed under uric-acid-responsive regulatory systemCodingCheng Li3252  . . . atatcaagcttatcgataccgtcgacctcg
BBa_K3027004Arabinose-inducible nuclease_A1 expression moduleCompositeArnaud Boudigou3161  . . . tttatctgttgtttgtcggtgaacgctctc
BBa_K3032076Ready to use mRFP1-CarH device (strong)DeviceDenis Baronas2000  . . . caccttcgggtgggcctttctgcgtttata
BBa_K3111502TmEncH_DARPin929_StrepII + miniSOG_TPCompositeMatas Deveikis2348  . . . gagagaccacgacgccggttactacattga
BBa_K3113302pCAG_Gag-HiBiT-L7AeProjectAlejandro Salinas Illarena3950  . . . ggcttctgaggcggaaagaaccagctgggg
BBa_K3128019COMP fused with T18 subpart of Bordetella Pertussis AC under constitutive promoterCompositePINERO Lucas1354  . . . caccttcgggtgggcctttctgcgtttata
BBa_K3134011T7-Cas1Cas2CompositeShiyuan Li1274  . . . ttaaggttggtgtcttttttacctgtttga
BBa_K3156888pLac Promoter-ssDNA[sfGFP(ON)]-Ec86-Beta CompositeTingzhen Liu2163  . . . tcggtgaacgctctcctgagtaggacaaat
BBa_K3168004dCas9-LargeBitNanoLucCompositeEva Hanckmann, Harm van der Veer, Claire Michielsen4680  . . . tcatggagccatccgcagtttgaaaaataa
BBa_K3182100pT7-CBDcipA-pCons-AsPinkCompositeOliver Hild Walett1334  . . . gcaccgagcaagctgggtcataattaataa
BBa_K3198007HicA-LuxABCDECompositeChunyang Song8199  . . . atgtttgccgatgcttttgcatacgtataa
BBa_K3264024TALEsp-vioA-vioB-vioC-vioEDeviceDiol Wang9346  . . . gtttggtacaagattggtcgcgtgaattga
BBa_K3376012ldhp-AQP-RBS-SpxB-Tr-tpxp-KatG-Tr/pSB1C3 CompositeLi, Sheng-Fong5168-1 . . . caccttcgggtgggcctttctgcgtttata
BBa_K3380500iSpinach fluorescent RNA aptamer construct under T7 RNA polymerase promoter (BBa_z0251)RNAAlexandru Popov183-1 . . . ctgatgatccttcgggatcattcatggcaa
BBa_K3431023zr31_ToeholdSwitch-Regulated InvertaseCompositeJian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang1474-1 . . . gcctctaaacgggtcttgaggggttttttg
BBa_K3440014GFP under Plux in presence of LuxR and AHLCompositeJulie Cordier1826-1 . . . catggcatggatgaactatacaaataataa
BBa_K3453111Rosewood DmMatK Toehold Switch 1.1 with sfGFP-LVAtagCompositeMaeva Cherriere931-1 . . . gctcttatcgggcggctaggggttttttgt
BBa_K3457039T7-RBS-SAHS 33020-T7-RBS-CAHS 106094CompositeYixian Yang1807-1 . . . gcctctaaacgggtcttgaggggttttttg
BBa_K3482017aTc and heat-inducible IM2 antitoxinCompositeThierry Marti523-1 . . . cagattattaatccggcttttttattattt
BBa_K3490001IPTG inducible NOS, over-express csgD and csgACompositeRyan Huang3929-1 . . . aataactctgatagtgctagtgtagatctc
BBa_K3596056BIOT with RBS mut4 and Pilin gene mut4ACompositeXinyu Liu8580-1 . . . ctggggtatctggcctggatctcttattaa
BBa_K3606029P2 driven mcbABCD and PtetR driven mcbEFGCompositeGaochen Zhang5255-1 . . . agggatgtcaatctctatcactgataggga
BBa_K3661002YebF-IL 22CompositeWeixuan Lu903-1 . . . atgtctctgagaaatgcctgcatttgataa
BBa_K3686012Chitinase from Xenorhabdus nematophila (strain 19061), complete CDs, codon-optimized.CodingYingJie Lei2000-1 . . . caactgccgaaggtcactcgccgcaagagc
BBa_K3728008ldhp-Phi29 DNA pol-Tr/pTol2CompositeEric Shih2080-1 . . . caccttcgggtgggcctttctgcgtttata
BBa_K3730012miRNA sponge, to reduce specific types of free hsa-miR-199a, 195 and 22 level in cellCompositeYongyin Wang294-1 . . . ggtctgaacactggggccaccatggaattc
BBa_K3733044Toxin/antitoxin HepT/MntA suicide system working at low temperaturesCompositeZhenhao Han1036-1 . . . gtgatttttgtcttcttgcgctaatttttt
BBa_K3736002Plux promoter + RBS + LL37 + RBS + mRFP + RBS + Terminator*2 CompositeYi Hua Li1884-1 . . . gactgtccacgacgctatacccaaaagaaa
BBa_K3739109K525998(T7-RBS)-LMT-his-CBM-hutH-B0010CompositeShichen Geng2157-1 . . . tttatctgttgtttgtcggtgaacgctctc
BBa_K3755041CMV enhancer+CMV promoter+GCaMP6m+SV40 PolyA signalCompositeKaijun Wang1983-1 . . . tggtttgtccaaactcatcaatgtatctta
BBa_K3782022cspA-RBS-TEE-His-FfIBP-TermCompositeJana Näf1036-1 . . . aatactagagtccctgccatttggcgggga
BBa_K3798032Fusion protein sequence comprised with two CBD domain and one eGFP domainCompositeHaohui Che1467-1 . . . caccttcgggtgggcctttctgcgtttata
BBa_K3799057Bidirectional AIP-1 sensorReporterShubhamay Das, Debdeep Chatterjee3167-1 . . . caccttcgggtgggcctttctgcgtttata
BBa_K3806016Theophylline-binding aptazyme regulating lacZ expression (semi-cRBS). With T7 promoter.CompositeLaura Sierra Heras3315-1 . . . gcctctaaacgggtcttgaggggttttttg
BBa_K3829013P-ss-PETase-V5tag-Anchor protein 5105-TCompositeJing Zhou3363-1 . . . atgccatacagtccctacttctaaaccaga
BBa_K3886003Caffeine SensorCompositeMeng Fankang5422-1 . . . cagattattaatccggcttttttattattt
BBa_K3890000Pollen expressed CYP6G1 and GUS reporter circuitCompositeBeatriz Toledo Akiti4157-1 . . . gaaaaaccgcagcagggaggcaaacaatga
BBa_K3895009kerBlMKU3_dna_pET-28a(+)CompositeRui Zhu1296-1 . . . caccttcgggtgggcctttctgcgtttata
BBa_K3898166A delicate modular enzymes system to enhance PET plastic degradation at mild temperatureCodingYuliang Huo4284-1 . . . caccttcgggtgggcctttctgcgtttata
BBa_K3904005Bile-regulated and cold-inducible VapXD kill-switchCompositeIta Čiutaitė934-1 . . . aaaaaccccgcttcggcggggttttttcgc
BBa_K3963001pBAV1k-lacI-Trc-beta-agarase YM01-3PlasmidSilja Malkewitz3636-1 . . . ggtaataatcagcagtggaaatttcagtaa
BBa_K3989011plant immune elicitor elf18 fused with ClyA for OMV displayCodingSong-Yuan Zhang1101-1 . . . aaaccgcacgttaacgttggtaccatcggt
BBa_K4130000IgG F(c) Binding Protein, EibDCompositeSarah Broas1799-1 . . . caccttcgggtgggcctttctgcgtttata
BBa_K4134066BpfA-Atox1-Linker-KanR-loxP-AggCDeviceJiankai Liu3078-1 . . . gctttacactctatacccaacccaaattaa
BBa_K4162117 ribozyme+RBS+CDS module: crtYEBIDNAWeiwen Chen4836-1 . . . ggtttgatgctggaggatctgatataataa
BBa_K4170016SUMO-LbuCas13a coding device under T7 promoterDeviceAlexandros Giannopoulos Dimitriou5404-1 . . . gaagagaagaaaagcgagaattaataataa
BBa_K4179020Luc_Blast_OraCellCompositeBaraah Rashed3291-1 . . . ctcatcaatgtatcttatcatgtctggatc
BBa_K4192121Plac-obcA-obcB-Plac-Fpoar, Oxalate secretion gene circuitCompositeMuhua Liu3750-1 . . . cgggaatggcggcaagaagatcttctctga
BBa_K4247025mCherry-SnoopCatcherCodingMatteo Soana1065-1 . . . tacattactaacgagccaattccgcctaag
BBa_K4271001T7 Promoter + Lac operator + RBS + OPH + T7 terminatorCompositeEthan Ho1307-1 . . . gcctctaaacgggtcttgaggggttttttg
BBa_K4273020pTDH3-Np5598-tTDH1-pPGK1-NlmysD-tPGK1CompositeSu Junzhe4772-1 . . . gtttttttttcccattcgatatttctatgt
BBa_K4365021SP-SUMO-turboRFPDeviceGiorgio Gilioli2582-1 . . . gttttgggacgctcgaaggctttaatttgc
BBa_K4400002InaK+TyrosinaseCodingXiangkai Li2025-1 . . . gtaggctctggccatcaccaccaccaccac


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