Difference between revisions of "Catalog"

Revision as of 00:46, 27 March 2009

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	Promoters (?): A promoter is a DNA sequence that tends to recruit transcriptional machinery and lead to transcription of the downstream DNA sequence.
	Ribosome Binding Sites (?): A ribosome binding site (RBS) is an RNA sequence found in mRNA to which ribosomes can bind and initiate translation.
	Protein tags and modifiers (?): Protein tags and modifiers are short peptide sequences cloned in frame with protein coding sequences that change the protein's behavior. Protein tags and modifiers might change the protein's location, alter its degradation rate, target the protein for cleavage, or enable it to be readily purified.
	Protein coding sequences (?): Protein coding sequences encode the amino acid sequence of a particular protein. Note that some protein coding sequences only encode a protein domain or half a protein. Others encode a full-length protein from start codon to stop codon. Coding sequences for gene expression reporters such as LacZ and GFP are also included here.
	Terminators (?): A terminator is an RNA sequence that usually occurs at the end of a gene or operon mRNA and causes transcription to stop.
	DNA (?): DNA parts provide functionality to the DNA itself. DNA parts include cloning sites, scars, primer binding sites, spacers, recombination sites, conjugative tranfer elements, transposons, origami, and aptamers.
	Plasmid backbones (?): A plasmid is a circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the cell independently of the chromosomal DNA. A plasmid backbone is defined as the plasmid sequence beginning with the BioBrick suffix, including the replication origin and antibiotic resistance marker, and ending with the BioBrick prefix.
	Plasmids (?): A plasmid is a circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the cell independently of the chromosomal DNA. If you're looking for a plasmid or vector to propagate or assemble plasmid backbones, please see the set of plasmid backbones. There are a few parts in the Registry that are only available as circular plasmids, not as parts in a plasmid backbone, you can find them here. Note that these plasmids largely do not conform to the BioBrick standard.
	Primers (?): A primer is a short single-stranded DNA sequences used as a starting point for PCR amplification or sequencing. Although primers are not actually available via the Registry distribution, we include commonly used primer sequences here.
[[1]]	Composite parts (?): Composite parts are combinations of of two or more BioBrick parts.

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We're in the process of developing new support for the specification of devices in the Registry. For the time being, please see the existing device tables below.

[[2]]	Protein generators (?):
[[3]]	Reporters (?):
[[4]]	Inverters (?):
[[5]]	Receivers and senders (?):
[[6]]	Measurement devices (?):

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Unless otherwise specified, most parts in the Registry work in Escherichia coli.

	Escherichia coli (?): Most parts in the Registry function in E. coli.
	Yeast (?): Yeast are simple eukaryotes.
	Bacteriophage T7 (?): Bacteriophage T7 is an obligate lytic phage of E. coli.
	Bacillus subtilis (?): Bacillus subtilis is a model gram-positive bacterium.

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Unless otherwise specified, most parts in the Registry comply with the original BioBrick assembly standard (also known as Assembly standard 10).

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Looking for a particular strain? You can find it here.

	Escherichia coli chassis (?): Most parts in the Registry operate in E. coli.
	Bacillus subtilis chassis (?): Bacillus subtilis is a model gram-positive bacterium.
	Cell-free chassis (?): In vitro transcription/translation systems can be useful for some synthetic biological systems.