Bacillus subtilis chassis

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Bacillus subtilis is gram-positive model organism. Thus, much is known about this organism. The genome of Bacillus subtilis strain 168 has been sequenced.

Although the current part collection for B. subtilis is small, many are now using B. subtilis as a candidate host for synthetic devices and systems. Please read more about the advantages and disadvantages of using B. subtilis as a chassis.

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2008ImperialCollegeiGEMTeamPhoto.png Currently, the Registry does not maintain a collection of Bacillus subtilis strains. However, the following strains have been recommended for use by the Imperial College 2008 iGEM team.

Bacillus subtilis strain 168

Bacillus subtilis strain 168 is one of the most commonly used in the laboratory and its specific genome has been sequenced. It tends to be the traditional gram-positive lab worhhorse. It is easy to transform, highly motile and generally easy to work with.

Several of the UK iGEM teams including Cambridge, Ediburgh, and Newcastle have made use of this strain as a chassis for their projects.

This chassis currently provides the basis for the B. subtilis chassis characterisation data

Bacillus subtilis strain Py79

Like Bacillus subtilis strain 168, Bacillus subtilis strain Py79 is also a lab strain of Bacillus subtilis. It is commonly used in research and also is a general workhorse strain.