Protein domains/Localization

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Certain protein tags can direct a protein to a particular physical location in the cell, such as the nucleus, the membrane, the periplasm, secretion outside of the cell or elsewhere. The localization tags are useful for achieving spatial segregation of proteins.


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NameDescriptionAA sequenceLength
BBa_I712009CD4 signal peptide; localizes to plasma membrane 75
BBa_I712016myristoilation signal sequence fused to Tobacco Etch Virus (TEV) protease cleavage site 57
BBa_I712026myristoilation signal sequence; anchors protein to membrane 21
BBa_J32015PelB leader sequence; directs protein to E. coli periplasmic membrane 66
BBa_J63007PKI nuclear export sequence; yeast codon optimized 30
BBa_J63008SV40 nuclear localization sequence from SV40; yeast codon optimized 21
BBa_J64008SicP-SptP chaperone-secretion tag pair from Salmonella type III secretion system 880
BBa_K103006OmpA outer membrane protein A fused to linker; displays proteins on cell surface 464
BBa_K1119000Mitochondrial Leader Sequence in RFC10 standard 87
BBa_K1119001Mitochondrial Leader Sequence with RFC25 standard 87
BBa_K112230{rbs.pelB} The pelB leader with native rbs; assembly standard 21 85
BBa_K125300pilA1 signal sequence from cyanobacterium Synechocystis; secretes protein 62
BBa_K125310slr2016 signal sequence from cyanobacterium Synechocystis; secretes protein 53
BBa_K128004signal sequence from Lactobacillus bulgaricus; secretes protein 143
BBa_K1478000Expa4 plant secretion signal, localizes to extracellular space 60
BBa_K157001EGFR/ErbB1 signal peptide; mediates protein transport to a translocational pore 72
BBa_K157010glycine-serine linker fused to B-cell receptor transmembrane region; displays protein on cell surfac 108
BBa_K1642007Ecolivk 69
BBa_K1642008Sll1338 81
BBa_K1680004SV40 NLS 24
BBa_K1680013NDegron 165
BBa_K1732000J23100-PelB-GlucHCO-B0015 794
BBa_K1909003EnvZ Signal Peptide and first Transmembrane Domain 161
BBa_K1936001Tom5-eGFP-LOV2 1368
BBa_K2429124Nuclear Localization Sequence (beginning) 27
BBa_K2429125Nuclear Localization Sequence (end) 30
BBa_K249004N-terminal Arginine fusion vector 33
BBa_K249005C-terminal Arginine Fusion Vector 33
BBa_K2549038CfaC-ZF21.16C-NLS 294
BBa_K2549039VP64-dNLS-ZF21.16 507
BBa_K2549040KRAB-dNLS-ZF21.16 582
BBa_K2549041NLS-TEVp 747
BBa_K2549042NLS-TEVpN-CfaN 678
BBa_K2549044CD8alpha signal peptide 63
BBa_K2549054SV40 NLS 21
BBa_K2549056destroyable nuclear location sequence 48
BBa_K2812004Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA 1458
BBa_K2812005Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter 1496
BBa_K2812006Coding sequence for Pyocin S5 with HlyA and His6-tag 2217
BBa_K2812007Coding sequence for Pyocin S5 with HlyA and His6-tag regulated by pBAD-ara promoter 2288
BBa_K283036invasin(1-625aa) 1900
BBa_K3016100Vibrio natriegens' TorA signal peptide 102
BBa_K3016101Vibrio natriegens' aminotransferase Tat signal peptide 117
BBa_K3016200Vibrio natriegens' TorA signal peptide containing YGFP gene 819
BBa_K3016210Vibrio natriegens' TorA signal peptide containing human growth hormone with 6xHis 816
BBa_K3114000Signal peptide from DsbA (secretion tag) 78
BBa_K3114001Signal peptide from MalE (secretion tag) 99
BBa_K3114002Signal peptide from OmpA (secretion tag) 84
BBa_K3114003Signal peptide from PhoA (secretion tag) 84
BBa_K3114004YcbK signal peptide (secretion tag) 111
BBa_K3114005Signal peptide from TorA (secretion tag) 138
BBa_K3765002PelB Signal Peptide 66
BBa_K3765007OpdA Signal Peptide 81
BBa_K392005yeast SUC2 leader sequence 57
BBa_K416000Aga2 CDS Responsible for Yeast Surface Display 261
BBa_K416003Yeast Secretion Tag 114
BBa_K416004Aga2:(Gly3Ser)4 Linker Composite Part 314
BBa_K416008Aga2 fused to eCFP 1835
BBa_K4359000E.coli K-12 Cytolysin A (ClyA)  1008
BBa_K4359001E.coli K-12 Cytolysin A (ClyA) (Y181F) 930
BBa_K4365006Signal peptide of MPGI from Magnaporthe grisea 81
BBa_K4365007Signal peptide of RodA from Aspergillus nidulans 129
BBa_K4365008Signal peptide of HYPI from Aspergillus fumigatus 84
BBa_K4365009Signal peptide of SsgA from Metarhizium anisopliae 63
BBa_K4365010Signal peptide of HCF-4 from Cladosporium fulvum 63
BBa_K4365011Signal peptide of HCF-1 from Cladosporium fulvum 66
BBa_K4380001OmpA signalling sequence 60
BBa_K5310000Igk signal peptide 57
BBa_K5466002AGA2P Signal Peptide 60
BBa_K5466006SUC2 Signal Peptide. Saccharomyces cerevisiae codon optimized 57
BBa_K678002eGFP-PTS1 for mammalian cells 3398
BBa_K678025Mitochondrial targeting sequence for Aspergillus nidulans 188
BBa_K678033GFP-PTS1 737
BBa_K678034mRFP1-NLS, targeted to the nucleus 707
BBa_K792002Secretion tag from yeast α-factor mating pheromone (MFα1) 54
BBa_K801030Polypeptide containing SV40 nuclear localization sequence (SV40 NLS) for nuclear translocation 57
BBa_K801039SV40NLS-GAL4AD-linker-PIF3 (part for GAL4/LexA based light-switchable promoter system) 804
BBa_K801040SV40NLS-PhyB-linker-Gal4DBD (part for GAL4 based light switchable promoter system) 3294
BBa_K801041SV40NLS-PhyB-linker-LexA (part for a LexA based light-switchable promoter system) 3459
BBa_K801042GAL4 based yeast light-switchable promoter system 6063
BBa_K801043LexA based yeast light-swithable promoter system 6228
BBa_K900002Nucleus Targeting Protein 408
BBa_K900003Vacuolar Membrane Targeting Protein 396
BBa_K900004Actin Targeting Protein 1779
BBa_K900005Plasma Membrane Targeting Sequence 12