Tag
pilA1 SS

Part:BBa_K125300

Designed by: Krystle Salazar and Grace Kwan   Group: iGEM08_Hawaii   (2008-07-25)

pilA1 signal sequence from cyanobacterium Synechocystis; secretes protein

pilA1, a pilin, is the major secreted protein of the cyanobacteria Synechocystis sp. PCC6803. Cyanobacterial pilin secretion pathways appear to be dependent on the overall positive charge of the leader signalling sequence, and not the actual amino acid sequence of the signal (Sergeyenko). When this signalling sequence is attached to the N-terminus of a polypeptide (or directly upstream of a protein-coding BioBrick part), the polypeptide should be secreted by Synechocystis sp. PCC6803 into the extracellular environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/cyanobacterium
//proteindomain/localization
Parameters
None