Tag

Part:BBa_K3765007

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)


OpdA Signal Peptide

27 amino acid native signal peptide from the OpdA enzyme from Agrobacterium tumefaciens which helps in extracellular secretion of an attached protein into the periplasmic space.

Usage and Biology

This part was used in our project to enable secretion of the OpdA-SpyTag002 family of fusion proteins to the periplasmic space for easier purification and minimized risk of intracellular aggregation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/head
//proteindomain/localization
Parameters
None