Other
MTS

Part:BBa_K678025

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)

Mitochondrial targeting sequence for Aspergillus nidulans

This part is a mitochondrial targeting sequence (MTS) for Aspergillus nidulans. The MTS is a DNA fragment encoding the N-terminal 60 amino acids of AcuJ from A. nidulans.


Usage and Biology

A MTS is usually required for the entry of proteins and intermediary metabolites into mitochondria. Placing this MTS at the N-terminal of a gene of interest should result in the localization of the protein into the mitochondria (1).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 127
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote
//direction/forward
//proteindomain/localization
Parameters
chassisAspergillus nidulans