Tag

Part:BBa_K1680004

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-17)

SV40 NLS

This part contains the protein coding sequence for the nuclear localisation signal of SV40 large T antigen (amino acids: PKKKRKV).

Fluorescence microscopy showed nuclear localisation of the fusion construct of this part with the fluorescent protein dronpa (BBa_K1680006) in comparison to the distribtuion of unaltered dronpa.

Team Tuebingen microscpy-dronpa.png Team Tuebingen microscpy-NLS-dronpa.png

Fluorescence, brightfield and overlay pictures of Saccharomyces cerevisiae dronpa expressing cells (top) and cells expressing a NLS-dronpa fusion from the pTUM104 plasmid (Part:BBa_K801004) grown overnight in SC medium containing 2% galactose.

Sequence and Features

This part is submitted in pSB1C3 with RFC25 standard. The part only sequence does therefore not contain a start and stop codon.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/eukaryote/yeast
//proteindomain/localization
Parameters
None